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Insights into the Mechanisms Underlying Ultraviolet-C Induced Resveratrol Metabolism in Grapevine (V. amurensis Rupr.) cv. "Tonghua-3".

Yin X, Singer SD, Qiao H, Liu Y, Jiao C, Wang H, Li Z, Fei Z, Wang Y, Fan C, Wang X - Front Plant Sci (2016)

Bottom Line: Tonghua-3 following UV-C treatment, to accelerate research into resveratrol metabolism.Comparative RNA-Seq profiling of UV-C treated and untreated grape berries resulted in the identification of a large number of differentially expressed genes.This transcriptome data set provides new insight into the response of grape berries to UV-C treatment, and suggests candidate genes, or promoter activity of related genes, that could be used in future functional and molecular biological studies of resveratrol metabolism.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Crop Stress Biology in Arid Areas, College of Horticulture, Northwest A&F UniversityYangling, China; Key Laboratory of Horticultural Plant Biology and Germplasm Innovation in Northwest China, Ministry of Agriculture, Northwest A&F UniversityYangling, China.

ABSTRACT
Stilbene compounds belong to a family of secondary metabolites that are derived from the phenylpropanoid pathway. Production of the stilbene phytoalexin, resveratrol, in grape (Vitis spp.) berries is known to be induced by ultraviolet-C radiation (UV-C), which has numerous regulatory effects on plant physiology. While previous studies have described changes in gene expression caused by UV-C light in several plant species, such information has yet to be reported for grapevine. We investigated both the resveratrol content and gene expression responses of berries from V. amurensis cv. Tonghua-3 following UV-C treatment, to accelerate research into resveratrol metabolism. Comparative RNA-Seq profiling of UV-C treated and untreated grape berries resulted in the identification of a large number of differentially expressed genes. Gene ontology (GO) term classification and biochemical pathway analyses suggested that UV-C treatment caused changes in various cellular processes, as well as in both hormone and secondary metabolism. The data further indicate that UV-C induced increases in resveratrol may be related to the transcriptional regulation of genes involved in the production of secondary metabolites and signaling, as well as several transcription factors. We also observed that following UV-C treatment, 22 stilbene synthase (STS) genes exhibited increases in their expression levels and a VaSTS promoter drove the expression of the GUS reporter gene when expressed in tobacco. We therefore propose that UV-C induction of VaSTS expression is an important factor in promoting resveratrol accumulation. This transcriptome data set provides new insight into the response of grape berries to UV-C treatment, and suggests candidate genes, or promoter activity of related genes, that could be used in future functional and molecular biological studies of resveratrol metabolism.

No MeSH data available.


Functional categorization of genes differentially expressed after UV-C treatment based on the “biological process” category of Gene Ontology (GO).
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Figure 3: Functional categorization of genes differentially expressed after UV-C treatment based on the “biological process” category of Gene Ontology (GO).

Mentions: A total of 662 of the 881 DEGs identified at 4 h post-treatment, and 824 of the 1058 DEGs identified at 24 h post-treatment, were assigned to at least one term within the GO categories of “molecular function,” “cellular component,” or “biological process.” These DEGs were further classified into 100 functional subcategories (Figure 3; Table S5).


Insights into the Mechanisms Underlying Ultraviolet-C Induced Resveratrol Metabolism in Grapevine (V. amurensis Rupr.) cv. "Tonghua-3".

Yin X, Singer SD, Qiao H, Liu Y, Jiao C, Wang H, Li Z, Fei Z, Wang Y, Fan C, Wang X - Front Plant Sci (2016)

Functional categorization of genes differentially expressed after UV-C treatment based on the “biological process” category of Gene Ontology (GO).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4835806&req=5

Figure 3: Functional categorization of genes differentially expressed after UV-C treatment based on the “biological process” category of Gene Ontology (GO).
Mentions: A total of 662 of the 881 DEGs identified at 4 h post-treatment, and 824 of the 1058 DEGs identified at 24 h post-treatment, were assigned to at least one term within the GO categories of “molecular function,” “cellular component,” or “biological process.” These DEGs were further classified into 100 functional subcategories (Figure 3; Table S5).

Bottom Line: Tonghua-3 following UV-C treatment, to accelerate research into resveratrol metabolism.Comparative RNA-Seq profiling of UV-C treated and untreated grape berries resulted in the identification of a large number of differentially expressed genes.This transcriptome data set provides new insight into the response of grape berries to UV-C treatment, and suggests candidate genes, or promoter activity of related genes, that could be used in future functional and molecular biological studies of resveratrol metabolism.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Crop Stress Biology in Arid Areas, College of Horticulture, Northwest A&F UniversityYangling, China; Key Laboratory of Horticultural Plant Biology and Germplasm Innovation in Northwest China, Ministry of Agriculture, Northwest A&F UniversityYangling, China.

ABSTRACT
Stilbene compounds belong to a family of secondary metabolites that are derived from the phenylpropanoid pathway. Production of the stilbene phytoalexin, resveratrol, in grape (Vitis spp.) berries is known to be induced by ultraviolet-C radiation (UV-C), which has numerous regulatory effects on plant physiology. While previous studies have described changes in gene expression caused by UV-C light in several plant species, such information has yet to be reported for grapevine. We investigated both the resveratrol content and gene expression responses of berries from V. amurensis cv. Tonghua-3 following UV-C treatment, to accelerate research into resveratrol metabolism. Comparative RNA-Seq profiling of UV-C treated and untreated grape berries resulted in the identification of a large number of differentially expressed genes. Gene ontology (GO) term classification and biochemical pathway analyses suggested that UV-C treatment caused changes in various cellular processes, as well as in both hormone and secondary metabolism. The data further indicate that UV-C induced increases in resveratrol may be related to the transcriptional regulation of genes involved in the production of secondary metabolites and signaling, as well as several transcription factors. We also observed that following UV-C treatment, 22 stilbene synthase (STS) genes exhibited increases in their expression levels and a VaSTS promoter drove the expression of the GUS reporter gene when expressed in tobacco. We therefore propose that UV-C induction of VaSTS expression is an important factor in promoting resveratrol accumulation. This transcriptome data set provides new insight into the response of grape berries to UV-C treatment, and suggests candidate genes, or promoter activity of related genes, that could be used in future functional and molecular biological studies of resveratrol metabolism.

No MeSH data available.