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Insights into the Mechanisms Underlying Ultraviolet-C Induced Resveratrol Metabolism in Grapevine (V. amurensis Rupr.) cv. "Tonghua-3".

Yin X, Singer SD, Qiao H, Liu Y, Jiao C, Wang H, Li Z, Fei Z, Wang Y, Fan C, Wang X - Front Plant Sci (2016)

Bottom Line: Tonghua-3 following UV-C treatment, to accelerate research into resveratrol metabolism.Comparative RNA-Seq profiling of UV-C treated and untreated grape berries resulted in the identification of a large number of differentially expressed genes.This transcriptome data set provides new insight into the response of grape berries to UV-C treatment, and suggests candidate genes, or promoter activity of related genes, that could be used in future functional and molecular biological studies of resveratrol metabolism.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Crop Stress Biology in Arid Areas, College of Horticulture, Northwest A&F UniversityYangling, China; Key Laboratory of Horticultural Plant Biology and Germplasm Innovation in Northwest China, Ministry of Agriculture, Northwest A&F UniversityYangling, China.

ABSTRACT
Stilbene compounds belong to a family of secondary metabolites that are derived from the phenylpropanoid pathway. Production of the stilbene phytoalexin, resveratrol, in grape (Vitis spp.) berries is known to be induced by ultraviolet-C radiation (UV-C), which has numerous regulatory effects on plant physiology. While previous studies have described changes in gene expression caused by UV-C light in several plant species, such information has yet to be reported for grapevine. We investigated both the resveratrol content and gene expression responses of berries from V. amurensis cv. Tonghua-3 following UV-C treatment, to accelerate research into resveratrol metabolism. Comparative RNA-Seq profiling of UV-C treated and untreated grape berries resulted in the identification of a large number of differentially expressed genes. Gene ontology (GO) term classification and biochemical pathway analyses suggested that UV-C treatment caused changes in various cellular processes, as well as in both hormone and secondary metabolism. The data further indicate that UV-C induced increases in resveratrol may be related to the transcriptional regulation of genes involved in the production of secondary metabolites and signaling, as well as several transcription factors. We also observed that following UV-C treatment, 22 stilbene synthase (STS) genes exhibited increases in their expression levels and a VaSTS promoter drove the expression of the GUS reporter gene when expressed in tobacco. We therefore propose that UV-C induction of VaSTS expression is an important factor in promoting resveratrol accumulation. This transcriptome data set provides new insight into the response of grape berries to UV-C treatment, and suggests candidate genes, or promoter activity of related genes, that could be used in future functional and molecular biological studies of resveratrol metabolism.

No MeSH data available.


Hierarchical cluster analysis of all transcripts identified through RNA-Seq analysis of UV-C treated (T) and untreated control (CK) berries 4 and 24 h following irradiation. Two biological replicates (1 and 2) were used in each case. Different shades of red and green express the extent of the change according to the color bar provided (log2 ratio of control); black indicates no change.
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Figure 2: Hierarchical cluster analysis of all transcripts identified through RNA-Seq analysis of UV-C treated (T) and untreated control (CK) berries 4 and 24 h following irradiation. Two biological replicates (1 and 2) were used in each case. Different shades of red and green express the extent of the change according to the color bar provided (log2 ratio of control); black indicates no change.

Mentions: Correlations between the 4 and 24 h post-treatment time points, along with the untreated control samples, were investigated using cluster analysis (Figure 2; Eisen et al., 1998). As expected, the 4h-CK and 24 h-CK samples (untreated controls) exhibited fewer differences in gene expression between them than did 4h-T and 24h-T samples (UV-C irradiated) (Figure 2). Interestingly, there were even more substantial differences between the two UV treated samples and their untreated controls, respectively, which correlated well with our resveratrol content results.


Insights into the Mechanisms Underlying Ultraviolet-C Induced Resveratrol Metabolism in Grapevine (V. amurensis Rupr.) cv. "Tonghua-3".

Yin X, Singer SD, Qiao H, Liu Y, Jiao C, Wang H, Li Z, Fei Z, Wang Y, Fan C, Wang X - Front Plant Sci (2016)

Hierarchical cluster analysis of all transcripts identified through RNA-Seq analysis of UV-C treated (T) and untreated control (CK) berries 4 and 24 h following irradiation. Two biological replicates (1 and 2) were used in each case. Different shades of red and green express the extent of the change according to the color bar provided (log2 ratio of control); black indicates no change.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4835806&req=5

Figure 2: Hierarchical cluster analysis of all transcripts identified through RNA-Seq analysis of UV-C treated (T) and untreated control (CK) berries 4 and 24 h following irradiation. Two biological replicates (1 and 2) were used in each case. Different shades of red and green express the extent of the change according to the color bar provided (log2 ratio of control); black indicates no change.
Mentions: Correlations between the 4 and 24 h post-treatment time points, along with the untreated control samples, were investigated using cluster analysis (Figure 2; Eisen et al., 1998). As expected, the 4h-CK and 24 h-CK samples (untreated controls) exhibited fewer differences in gene expression between them than did 4h-T and 24h-T samples (UV-C irradiated) (Figure 2). Interestingly, there were even more substantial differences between the two UV treated samples and their untreated controls, respectively, which correlated well with our resveratrol content results.

Bottom Line: Tonghua-3 following UV-C treatment, to accelerate research into resveratrol metabolism.Comparative RNA-Seq profiling of UV-C treated and untreated grape berries resulted in the identification of a large number of differentially expressed genes.This transcriptome data set provides new insight into the response of grape berries to UV-C treatment, and suggests candidate genes, or promoter activity of related genes, that could be used in future functional and molecular biological studies of resveratrol metabolism.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Crop Stress Biology in Arid Areas, College of Horticulture, Northwest A&F UniversityYangling, China; Key Laboratory of Horticultural Plant Biology and Germplasm Innovation in Northwest China, Ministry of Agriculture, Northwest A&F UniversityYangling, China.

ABSTRACT
Stilbene compounds belong to a family of secondary metabolites that are derived from the phenylpropanoid pathway. Production of the stilbene phytoalexin, resveratrol, in grape (Vitis spp.) berries is known to be induced by ultraviolet-C radiation (UV-C), which has numerous regulatory effects on plant physiology. While previous studies have described changes in gene expression caused by UV-C light in several plant species, such information has yet to be reported for grapevine. We investigated both the resveratrol content and gene expression responses of berries from V. amurensis cv. Tonghua-3 following UV-C treatment, to accelerate research into resveratrol metabolism. Comparative RNA-Seq profiling of UV-C treated and untreated grape berries resulted in the identification of a large number of differentially expressed genes. Gene ontology (GO) term classification and biochemical pathway analyses suggested that UV-C treatment caused changes in various cellular processes, as well as in both hormone and secondary metabolism. The data further indicate that UV-C induced increases in resveratrol may be related to the transcriptional regulation of genes involved in the production of secondary metabolites and signaling, as well as several transcription factors. We also observed that following UV-C treatment, 22 stilbene synthase (STS) genes exhibited increases in their expression levels and a VaSTS promoter drove the expression of the GUS reporter gene when expressed in tobacco. We therefore propose that UV-C induction of VaSTS expression is an important factor in promoting resveratrol accumulation. This transcriptome data set provides new insight into the response of grape berries to UV-C treatment, and suggests candidate genes, or promoter activity of related genes, that could be used in future functional and molecular biological studies of resveratrol metabolism.

No MeSH data available.