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Gene set of chemosensory receptors in the polyembryonic endoparasitoid Macrocentrus cingulum.

Ahmed T, Zhang T, Wang Z, He K, Bai S - Sci Rep (2016)

Bottom Line: Insects are extremely successful animals whose odor perception is very prominent due to their sophisticated olfactory system.The sequences of all of these transcripts were confirmed by RT-PCR, and direct DNA sequencing.This reported result provides a comprehensive resource of the foundation in semiochemicals driven behaviors at molecular level in polyembryonic endoparasitoid.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory for the Biology of the Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.

ABSTRACT
Insects are extremely successful animals whose odor perception is very prominent due to their sophisticated olfactory system. The main chemosensory organ, antennae play a critical role in detecting odor in ambient environment before initiating appropriate behavioral responses. The antennal chemosensory receptor genes families have been suggested to be involved in olfactory signal transduction pathway as a sensory neuron response. The Macrocentrus cingulum is deployed successfully as a biological control agent for corn pest insects from the Lepidopteran genus Ostrinia. In this research, we assembled antennal transcriptomes of M. cingulum by using next generation sequencing to identify the major chemosensory receptors gene families. In total, 112 olfactory receptors candidates (79 odorant receptors, 20 gustatory receptors, and 13 ionotropic receptors) have been identified from the male and female antennal transcriptome. The sequences of all of these transcripts were confirmed by RT-PCR, and direct DNA sequencing. Expression profiles of gustatory receptors in olfactory and non-olfactory tissues were measured by RT-qPCR. The sex-specific and sex-biased chemoreceptors expression patterns suggested that they may have important functions in sense detection which behaviorally relevant to odor molecules. This reported result provides a comprehensive resource of the foundation in semiochemicals driven behaviors at molecular level in polyembryonic endoparasitoid.

No MeSH data available.


Related in: MedlinePlus

Expression patterns of McinGR genes.Transcript levels of M. cingulum gustatory receptors (GRs) in different tissues of adult male and females measured by qPCR. cDNAs were amplified with specific primers from antennae, heads (with mouth parts), legs, and bodies.
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f9: Expression patterns of McinGR genes.Transcript levels of M. cingulum gustatory receptors (GRs) in different tissues of adult male and females measured by qPCR. cDNAs were amplified with specific primers from antennae, heads (with mouth parts), legs, and bodies.

Mentions: The quantitative real-time PCR (qPCR) was used to investigate the gustatory receptor transcript abundances in the male antennae, head with mouth parts, legs, body and female antennae, head with mouth parts, legs and body tissues. By comparing expression levels McinGR10, 13, 14, 17, 18 and 19 genes were expressed at similar level in all tested tissues except 14, 18, and 19 in body of both sexes (Fig. 9). McinGR1, 3, 4, 15 and 20 were prominently expressed only male antennae and legs where as McinGR6, 8 and 11 in male antennae, legs and female legs but 16 dominantly expressed only in male legs (Fig. 9). McinGR2, 7, and 9 were highly expressed in female antenna than male. McinGR5 were highly expressed in male antenna than female.


Gene set of chemosensory receptors in the polyembryonic endoparasitoid Macrocentrus cingulum.

Ahmed T, Zhang T, Wang Z, He K, Bai S - Sci Rep (2016)

Expression patterns of McinGR genes.Transcript levels of M. cingulum gustatory receptors (GRs) in different tissues of adult male and females measured by qPCR. cDNAs were amplified with specific primers from antennae, heads (with mouth parts), legs, and bodies.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4835793&req=5

f9: Expression patterns of McinGR genes.Transcript levels of M. cingulum gustatory receptors (GRs) in different tissues of adult male and females measured by qPCR. cDNAs were amplified with specific primers from antennae, heads (with mouth parts), legs, and bodies.
Mentions: The quantitative real-time PCR (qPCR) was used to investigate the gustatory receptor transcript abundances in the male antennae, head with mouth parts, legs, body and female antennae, head with mouth parts, legs and body tissues. By comparing expression levels McinGR10, 13, 14, 17, 18 and 19 genes were expressed at similar level in all tested tissues except 14, 18, and 19 in body of both sexes (Fig. 9). McinGR1, 3, 4, 15 and 20 were prominently expressed only male antennae and legs where as McinGR6, 8 and 11 in male antennae, legs and female legs but 16 dominantly expressed only in male legs (Fig. 9). McinGR2, 7, and 9 were highly expressed in female antenna than male. McinGR5 were highly expressed in male antenna than female.

Bottom Line: Insects are extremely successful animals whose odor perception is very prominent due to their sophisticated olfactory system.The sequences of all of these transcripts were confirmed by RT-PCR, and direct DNA sequencing.This reported result provides a comprehensive resource of the foundation in semiochemicals driven behaviors at molecular level in polyembryonic endoparasitoid.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory for the Biology of the Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.

ABSTRACT
Insects are extremely successful animals whose odor perception is very prominent due to their sophisticated olfactory system. The main chemosensory organ, antennae play a critical role in detecting odor in ambient environment before initiating appropriate behavioral responses. The antennal chemosensory receptor genes families have been suggested to be involved in olfactory signal transduction pathway as a sensory neuron response. The Macrocentrus cingulum is deployed successfully as a biological control agent for corn pest insects from the Lepidopteran genus Ostrinia. In this research, we assembled antennal transcriptomes of M. cingulum by using next generation sequencing to identify the major chemosensory receptors gene families. In total, 112 olfactory receptors candidates (79 odorant receptors, 20 gustatory receptors, and 13 ionotropic receptors) have been identified from the male and female antennal transcriptome. The sequences of all of these transcripts were confirmed by RT-PCR, and direct DNA sequencing. Expression profiles of gustatory receptors in olfactory and non-olfactory tissues were measured by RT-qPCR. The sex-specific and sex-biased chemoreceptors expression patterns suggested that they may have important functions in sense detection which behaviorally relevant to odor molecules. This reported result provides a comprehensive resource of the foundation in semiochemicals driven behaviors at molecular level in polyembryonic endoparasitoid.

No MeSH data available.


Related in: MedlinePlus