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Gene set of chemosensory receptors in the polyembryonic endoparasitoid Macrocentrus cingulum.

Ahmed T, Zhang T, Wang Z, He K, Bai S - Sci Rep (2016)

Bottom Line: Insects are extremely successful animals whose odor perception is very prominent due to their sophisticated olfactory system.The sequences of all of these transcripts were confirmed by RT-PCR, and direct DNA sequencing.This reported result provides a comprehensive resource of the foundation in semiochemicals driven behaviors at molecular level in polyembryonic endoparasitoid.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory for the Biology of the Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.

ABSTRACT
Insects are extremely successful animals whose odor perception is very prominent due to their sophisticated olfactory system. The main chemosensory organ, antennae play a critical role in detecting odor in ambient environment before initiating appropriate behavioral responses. The antennal chemosensory receptor genes families have been suggested to be involved in olfactory signal transduction pathway as a sensory neuron response. The Macrocentrus cingulum is deployed successfully as a biological control agent for corn pest insects from the Lepidopteran genus Ostrinia. In this research, we assembled antennal transcriptomes of M. cingulum by using next generation sequencing to identify the major chemosensory receptors gene families. In total, 112 olfactory receptors candidates (79 odorant receptors, 20 gustatory receptors, and 13 ionotropic receptors) have been identified from the male and female antennal transcriptome. The sequences of all of these transcripts were confirmed by RT-PCR, and direct DNA sequencing. Expression profiles of gustatory receptors in olfactory and non-olfactory tissues were measured by RT-qPCR. The sex-specific and sex-biased chemoreceptors expression patterns suggested that they may have important functions in sense detection which behaviorally relevant to odor molecules. This reported result provides a comprehensive resource of the foundation in semiochemicals driven behaviors at molecular level in polyembryonic endoparasitoid.

No MeSH data available.


Related in: MedlinePlus

Tissue- and sex- specific expression profiles of M. cingulum GRs and IRs.Comparison of expression profile of gustatory receptor (GR) and ionotropic receptors (IR) genes in male and female adult antennae and head with mouth parts, leg and body as revealed by RT-PCR. In each box, the relative abundance value in (M. pixel) of each receptor gene is indicated. Color scales were established using the conditional formatting option in Excel (color scale shown inside the figure).
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f8: Tissue- and sex- specific expression profiles of M. cingulum GRs and IRs.Comparison of expression profile of gustatory receptor (GR) and ionotropic receptors (IR) genes in male and female adult antennae and head with mouth parts, leg and body as revealed by RT-PCR. In each box, the relative abundance value in (M. pixel) of each receptor gene is indicated. Color scales were established using the conditional formatting option in Excel (color scale shown inside the figure).

Mentions: We performed reverse transcription PCR (RT-PCR) analyses in different tissues of adult males and females to explore the expression patterns of M. cingulum OR, GR and IR genes. Most of OR genes were expressed in male and female antennae, the crucial chemosensory organs, suggesting a functional role of these genes in olfaction (Fig. 7). The candidate OR31, 59, 62, 64, and 65 showed a male antenna specific expression, while only one OR25 was expressed only in female antennae. The remaining ORs were expressed in both sexes, by differential expressions in male or female among tissues. Five of them, OR11, 14, 54, 55 and 81 were most highly (>200 M. pixel) expressed in both male and female antennae (Fig. 7). Twenty-one ORs (OR18, 19, 22, 35, 38, 41, 43, 46, 48, 49, 50, 52, 53, 57, 60, 63, 67, 69, 70, 79, and 82) were clearly expressed higher in male compared to female while only two (OR27 and 40) expressed higher level in female than male. GRs and IRs showed a ubiquitous expression pattern except the McinGR12, 14 and 15 which was present predominantly in the male legs; McinIR64a prominent in male and female antennae but IR8a and 25a only in male antennae. McinIR76b dominantly expressed in male leg (Fig. 8).


Gene set of chemosensory receptors in the polyembryonic endoparasitoid Macrocentrus cingulum.

Ahmed T, Zhang T, Wang Z, He K, Bai S - Sci Rep (2016)

Tissue- and sex- specific expression profiles of M. cingulum GRs and IRs.Comparison of expression profile of gustatory receptor (GR) and ionotropic receptors (IR) genes in male and female adult antennae and head with mouth parts, leg and body as revealed by RT-PCR. In each box, the relative abundance value in (M. pixel) of each receptor gene is indicated. Color scales were established using the conditional formatting option in Excel (color scale shown inside the figure).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4835793&req=5

f8: Tissue- and sex- specific expression profiles of M. cingulum GRs and IRs.Comparison of expression profile of gustatory receptor (GR) and ionotropic receptors (IR) genes in male and female adult antennae and head with mouth parts, leg and body as revealed by RT-PCR. In each box, the relative abundance value in (M. pixel) of each receptor gene is indicated. Color scales were established using the conditional formatting option in Excel (color scale shown inside the figure).
Mentions: We performed reverse transcription PCR (RT-PCR) analyses in different tissues of adult males and females to explore the expression patterns of M. cingulum OR, GR and IR genes. Most of OR genes were expressed in male and female antennae, the crucial chemosensory organs, suggesting a functional role of these genes in olfaction (Fig. 7). The candidate OR31, 59, 62, 64, and 65 showed a male antenna specific expression, while only one OR25 was expressed only in female antennae. The remaining ORs were expressed in both sexes, by differential expressions in male or female among tissues. Five of them, OR11, 14, 54, 55 and 81 were most highly (>200 M. pixel) expressed in both male and female antennae (Fig. 7). Twenty-one ORs (OR18, 19, 22, 35, 38, 41, 43, 46, 48, 49, 50, 52, 53, 57, 60, 63, 67, 69, 70, 79, and 82) were clearly expressed higher in male compared to female while only two (OR27 and 40) expressed higher level in female than male. GRs and IRs showed a ubiquitous expression pattern except the McinGR12, 14 and 15 which was present predominantly in the male legs; McinIR64a prominent in male and female antennae but IR8a and 25a only in male antennae. McinIR76b dominantly expressed in male leg (Fig. 8).

Bottom Line: Insects are extremely successful animals whose odor perception is very prominent due to their sophisticated olfactory system.The sequences of all of these transcripts were confirmed by RT-PCR, and direct DNA sequencing.This reported result provides a comprehensive resource of the foundation in semiochemicals driven behaviors at molecular level in polyembryonic endoparasitoid.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory for the Biology of the Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.

ABSTRACT
Insects are extremely successful animals whose odor perception is very prominent due to their sophisticated olfactory system. The main chemosensory organ, antennae play a critical role in detecting odor in ambient environment before initiating appropriate behavioral responses. The antennal chemosensory receptor genes families have been suggested to be involved in olfactory signal transduction pathway as a sensory neuron response. The Macrocentrus cingulum is deployed successfully as a biological control agent for corn pest insects from the Lepidopteran genus Ostrinia. In this research, we assembled antennal transcriptomes of M. cingulum by using next generation sequencing to identify the major chemosensory receptors gene families. In total, 112 olfactory receptors candidates (79 odorant receptors, 20 gustatory receptors, and 13 ionotropic receptors) have been identified from the male and female antennal transcriptome. The sequences of all of these transcripts were confirmed by RT-PCR, and direct DNA sequencing. Expression profiles of gustatory receptors in olfactory and non-olfactory tissues were measured by RT-qPCR. The sex-specific and sex-biased chemoreceptors expression patterns suggested that they may have important functions in sense detection which behaviorally relevant to odor molecules. This reported result provides a comprehensive resource of the foundation in semiochemicals driven behaviors at molecular level in polyembryonic endoparasitoid.

No MeSH data available.


Related in: MedlinePlus