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Gene set of chemosensory receptors in the polyembryonic endoparasitoid Macrocentrus cingulum.

Ahmed T, Zhang T, Wang Z, He K, Bai S - Sci Rep (2016)

Bottom Line: Insects are extremely successful animals whose odor perception is very prominent due to their sophisticated olfactory system.The sequences of all of these transcripts were confirmed by RT-PCR, and direct DNA sequencing.This reported result provides a comprehensive resource of the foundation in semiochemicals driven behaviors at molecular level in polyembryonic endoparasitoid.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory for the Biology of the Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.

ABSTRACT
Insects are extremely successful animals whose odor perception is very prominent due to their sophisticated olfactory system. The main chemosensory organ, antennae play a critical role in detecting odor in ambient environment before initiating appropriate behavioral responses. The antennal chemosensory receptor genes families have been suggested to be involved in olfactory signal transduction pathway as a sensory neuron response. The Macrocentrus cingulum is deployed successfully as a biological control agent for corn pest insects from the Lepidopteran genus Ostrinia. In this research, we assembled antennal transcriptomes of M. cingulum by using next generation sequencing to identify the major chemosensory receptors gene families. In total, 112 olfactory receptors candidates (79 odorant receptors, 20 gustatory receptors, and 13 ionotropic receptors) have been identified from the male and female antennal transcriptome. The sequences of all of these transcripts were confirmed by RT-PCR, and direct DNA sequencing. Expression profiles of gustatory receptors in olfactory and non-olfactory tissues were measured by RT-qPCR. The sex-specific and sex-biased chemoreceptors expression patterns suggested that they may have important functions in sense detection which behaviorally relevant to odor molecules. This reported result provides a comprehensive resource of the foundation in semiochemicals driven behaviors at molecular level in polyembryonic endoparasitoid.

No MeSH data available.


Related in: MedlinePlus

Phylogenetic tree based on protein sequences of ionotropic receptors (IRs).Included are IRs from M. cingulum, M. mediator (Mmed), A. mellifera (Amel), D. melanogaster (Dmel) and N. vitripennis (Nvit).
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f6: Phylogenetic tree based on protein sequences of ionotropic receptors (IRs).Included are IRs from M. cingulum, M. mediator (Mmed), A. mellifera (Amel), D. melanogaster (Dmel) and N. vitripennis (Nvit).

Mentions: We identified 13 transcripts for putative ionotropic receptors in M. cingulum antennal transcriptome according to their similarity to IR sequence of other insects. Comparative analysis revealed that one candidate IR (MmedIR8a) was deemed as IR8a homolog to its high identity (71%). IR25a and IR76b shared 57% and 46% identity with MmedIR25a.1 and MmedIR76b, respectively. It has been reported that, the above three genes (IR8a, IR25a and IR76b) are thought to play function as IR co-receptors538. In the phylogenetic tree of IRs, all McinIR candidates clustered with their ionotropic receptor orthologs into separate sub-clades (Fig. 6). Because of the relative high conservation of IRs, all the splits of McinIRs were strongly supported by high support values. The candidate IR unigenes were named according to their similarity to known IRs. The information, including unigene reference, length, and best blastx hit of all IRs were listed in Table 6.


Gene set of chemosensory receptors in the polyembryonic endoparasitoid Macrocentrus cingulum.

Ahmed T, Zhang T, Wang Z, He K, Bai S - Sci Rep (2016)

Phylogenetic tree based on protein sequences of ionotropic receptors (IRs).Included are IRs from M. cingulum, M. mediator (Mmed), A. mellifera (Amel), D. melanogaster (Dmel) and N. vitripennis (Nvit).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4835793&req=5

f6: Phylogenetic tree based on protein sequences of ionotropic receptors (IRs).Included are IRs from M. cingulum, M. mediator (Mmed), A. mellifera (Amel), D. melanogaster (Dmel) and N. vitripennis (Nvit).
Mentions: We identified 13 transcripts for putative ionotropic receptors in M. cingulum antennal transcriptome according to their similarity to IR sequence of other insects. Comparative analysis revealed that one candidate IR (MmedIR8a) was deemed as IR8a homolog to its high identity (71%). IR25a and IR76b shared 57% and 46% identity with MmedIR25a.1 and MmedIR76b, respectively. It has been reported that, the above three genes (IR8a, IR25a and IR76b) are thought to play function as IR co-receptors538. In the phylogenetic tree of IRs, all McinIR candidates clustered with their ionotropic receptor orthologs into separate sub-clades (Fig. 6). Because of the relative high conservation of IRs, all the splits of McinIRs were strongly supported by high support values. The candidate IR unigenes were named according to their similarity to known IRs. The information, including unigene reference, length, and best blastx hit of all IRs were listed in Table 6.

Bottom Line: Insects are extremely successful animals whose odor perception is very prominent due to their sophisticated olfactory system.The sequences of all of these transcripts were confirmed by RT-PCR, and direct DNA sequencing.This reported result provides a comprehensive resource of the foundation in semiochemicals driven behaviors at molecular level in polyembryonic endoparasitoid.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory for the Biology of the Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.

ABSTRACT
Insects are extremely successful animals whose odor perception is very prominent due to their sophisticated olfactory system. The main chemosensory organ, antennae play a critical role in detecting odor in ambient environment before initiating appropriate behavioral responses. The antennal chemosensory receptor genes families have been suggested to be involved in olfactory signal transduction pathway as a sensory neuron response. The Macrocentrus cingulum is deployed successfully as a biological control agent for corn pest insects from the Lepidopteran genus Ostrinia. In this research, we assembled antennal transcriptomes of M. cingulum by using next generation sequencing to identify the major chemosensory receptors gene families. In total, 112 olfactory receptors candidates (79 odorant receptors, 20 gustatory receptors, and 13 ionotropic receptors) have been identified from the male and female antennal transcriptome. The sequences of all of these transcripts were confirmed by RT-PCR, and direct DNA sequencing. Expression profiles of gustatory receptors in olfactory and non-olfactory tissues were measured by RT-qPCR. The sex-specific and sex-biased chemoreceptors expression patterns suggested that they may have important functions in sense detection which behaviorally relevant to odor molecules. This reported result provides a comprehensive resource of the foundation in semiochemicals driven behaviors at molecular level in polyembryonic endoparasitoid.

No MeSH data available.


Related in: MedlinePlus