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Gene set of chemosensory receptors in the polyembryonic endoparasitoid Macrocentrus cingulum.

Ahmed T, Zhang T, Wang Z, He K, Bai S - Sci Rep (2016)

Bottom Line: Insects are extremely successful animals whose odor perception is very prominent due to their sophisticated olfactory system.The sequences of all of these transcripts were confirmed by RT-PCR, and direct DNA sequencing.This reported result provides a comprehensive resource of the foundation in semiochemicals driven behaviors at molecular level in polyembryonic endoparasitoid.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory for the Biology of the Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.

ABSTRACT
Insects are extremely successful animals whose odor perception is very prominent due to their sophisticated olfactory system. The main chemosensory organ, antennae play a critical role in detecting odor in ambient environment before initiating appropriate behavioral responses. The antennal chemosensory receptor genes families have been suggested to be involved in olfactory signal transduction pathway as a sensory neuron response. The Macrocentrus cingulum is deployed successfully as a biological control agent for corn pest insects from the Lepidopteran genus Ostrinia. In this research, we assembled antennal transcriptomes of M. cingulum by using next generation sequencing to identify the major chemosensory receptors gene families. In total, 112 olfactory receptors candidates (79 odorant receptors, 20 gustatory receptors, and 13 ionotropic receptors) have been identified from the male and female antennal transcriptome. The sequences of all of these transcripts were confirmed by RT-PCR, and direct DNA sequencing. Expression profiles of gustatory receptors in olfactory and non-olfactory tissues were measured by RT-qPCR. The sex-specific and sex-biased chemoreceptors expression patterns suggested that they may have important functions in sense detection which behaviorally relevant to odor molecules. This reported result provides a comprehensive resource of the foundation in semiochemicals driven behaviors at molecular level in polyembryonic endoparasitoid.

No MeSH data available.


Related in: MedlinePlus

Phylogenetic tree of gustatory receptors (GRs).Included are candidate M. cingulum GRs with A. mellifera (Amel), D. melanogaster (Dmel) and N. vitripennis (Nvit) GRs.
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f5: Phylogenetic tree of gustatory receptors (GRs).Included are candidate M. cingulum GRs with A. mellifera (Amel), D. melanogaster (Dmel) and N. vitripennis (Nvit) GRs.

Mentions: We identified 20 transcripts encoding candidate GRs in the M. cingulum antennal transcriptome (Table 5). Most of candidate McinGRs were partial transcripts (only six represents full length protein), encoding overlapping but distinct sequences. This shows individual genes though it’s being fragment of protein sequence. A phylogeny was built with these 20 McinGRs, N. vitripennis, A. mellifera and D. melanogaster (Fig. 5). Based on the phylogenetic analysis, McinGRs were also observed to group with their presumed Drosophila orthologues, which have been shown to have roles in carbon dioxide detection (GR21a and GR63a)5758 and are members of the candidate sugar GR64 receptor subfamily (GR64e)59 or bitter (DmelGR93a)60Drosophila receptors (Fig. 5).


Gene set of chemosensory receptors in the polyembryonic endoparasitoid Macrocentrus cingulum.

Ahmed T, Zhang T, Wang Z, He K, Bai S - Sci Rep (2016)

Phylogenetic tree of gustatory receptors (GRs).Included are candidate M. cingulum GRs with A. mellifera (Amel), D. melanogaster (Dmel) and N. vitripennis (Nvit) GRs.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4835793&req=5

f5: Phylogenetic tree of gustatory receptors (GRs).Included are candidate M. cingulum GRs with A. mellifera (Amel), D. melanogaster (Dmel) and N. vitripennis (Nvit) GRs.
Mentions: We identified 20 transcripts encoding candidate GRs in the M. cingulum antennal transcriptome (Table 5). Most of candidate McinGRs were partial transcripts (only six represents full length protein), encoding overlapping but distinct sequences. This shows individual genes though it’s being fragment of protein sequence. A phylogeny was built with these 20 McinGRs, N. vitripennis, A. mellifera and D. melanogaster (Fig. 5). Based on the phylogenetic analysis, McinGRs were also observed to group with their presumed Drosophila orthologues, which have been shown to have roles in carbon dioxide detection (GR21a and GR63a)5758 and are members of the candidate sugar GR64 receptor subfamily (GR64e)59 or bitter (DmelGR93a)60Drosophila receptors (Fig. 5).

Bottom Line: Insects are extremely successful animals whose odor perception is very prominent due to their sophisticated olfactory system.The sequences of all of these transcripts were confirmed by RT-PCR, and direct DNA sequencing.This reported result provides a comprehensive resource of the foundation in semiochemicals driven behaviors at molecular level in polyembryonic endoparasitoid.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory for the Biology of the Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.

ABSTRACT
Insects are extremely successful animals whose odor perception is very prominent due to their sophisticated olfactory system. The main chemosensory organ, antennae play a critical role in detecting odor in ambient environment before initiating appropriate behavioral responses. The antennal chemosensory receptor genes families have been suggested to be involved in olfactory signal transduction pathway as a sensory neuron response. The Macrocentrus cingulum is deployed successfully as a biological control agent for corn pest insects from the Lepidopteran genus Ostrinia. In this research, we assembled antennal transcriptomes of M. cingulum by using next generation sequencing to identify the major chemosensory receptors gene families. In total, 112 olfactory receptors candidates (79 odorant receptors, 20 gustatory receptors, and 13 ionotropic receptors) have been identified from the male and female antennal transcriptome. The sequences of all of these transcripts were confirmed by RT-PCR, and direct DNA sequencing. Expression profiles of gustatory receptors in olfactory and non-olfactory tissues were measured by RT-qPCR. The sex-specific and sex-biased chemoreceptors expression patterns suggested that they may have important functions in sense detection which behaviorally relevant to odor molecules. This reported result provides a comprehensive resource of the foundation in semiochemicals driven behaviors at molecular level in polyembryonic endoparasitoid.

No MeSH data available.


Related in: MedlinePlus