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Gene set of chemosensory receptors in the polyembryonic endoparasitoid Macrocentrus cingulum.

Ahmed T, Zhang T, Wang Z, He K, Bai S - Sci Rep (2016)

Bottom Line: Insects are extremely successful animals whose odor perception is very prominent due to their sophisticated olfactory system.The sequences of all of these transcripts were confirmed by RT-PCR, and direct DNA sequencing.This reported result provides a comprehensive resource of the foundation in semiochemicals driven behaviors at molecular level in polyembryonic endoparasitoid.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory for the Biology of the Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.

ABSTRACT
Insects are extremely successful animals whose odor perception is very prominent due to their sophisticated olfactory system. The main chemosensory organ, antennae play a critical role in detecting odor in ambient environment before initiating appropriate behavioral responses. The antennal chemosensory receptor genes families have been suggested to be involved in olfactory signal transduction pathway as a sensory neuron response. The Macrocentrus cingulum is deployed successfully as a biological control agent for corn pest insects from the Lepidopteran genus Ostrinia. In this research, we assembled antennal transcriptomes of M. cingulum by using next generation sequencing to identify the major chemosensory receptors gene families. In total, 112 olfactory receptors candidates (79 odorant receptors, 20 gustatory receptors, and 13 ionotropic receptors) have been identified from the male and female antennal transcriptome. The sequences of all of these transcripts were confirmed by RT-PCR, and direct DNA sequencing. Expression profiles of gustatory receptors in olfactory and non-olfactory tissues were measured by RT-qPCR. The sex-specific and sex-biased chemoreceptors expression patterns suggested that they may have important functions in sense detection which behaviorally relevant to odor molecules. This reported result provides a comprehensive resource of the foundation in semiochemicals driven behaviors at molecular level in polyembryonic endoparasitoid.

No MeSH data available.


Related in: MedlinePlus

Phylogenetic tree of odorant receptors (ORs).Included are ORs from M. cingulum (Mcin), M. mediator (Mmed), and N. vitripennis (Nvit).
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f4: Phylogenetic tree of odorant receptors (ORs).Included are ORs from M. cingulum (Mcin), M. mediator (Mmed), and N. vitripennis (Nvit).

Mentions: With exception of Orco, the predicted ORs shared quite low identity probably due to the high variance among OR gene family. Only three of 79 ORs (McinOR12, McinOR15, and McinOR42) showed more than 50% identity with known ORs in NCBI database (Table 4). The phylogenetic analysis also showed that ORs were extremely divergent between species but formed monophyletic group within same species (Fig. 4). However the highly conserved Orco shared 95–99% amino acid sequence identity and clustered in same branch with orthologous relation among three species (Fig. 4). All of the other McinORs were distributed in different branches of the phylogenetic tree. Eight species specific subgroups were identified consisting of different numbers of McinORs. The highest 14 McinORs (OR11, 24, 36, 52, 55, 56, 67, 68, 74, 78, 80, 81, 85, and 86) and the lowest three McinORs (OR30, 60 and 84) and another three McinORs (OR1, 7 and 59) clustered within the species specific subgroup. Others McinOR are clustered with N. vitripennis or M. mediator ORs.


Gene set of chemosensory receptors in the polyembryonic endoparasitoid Macrocentrus cingulum.

Ahmed T, Zhang T, Wang Z, He K, Bai S - Sci Rep (2016)

Phylogenetic tree of odorant receptors (ORs).Included are ORs from M. cingulum (Mcin), M. mediator (Mmed), and N. vitripennis (Nvit).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4835793&req=5

f4: Phylogenetic tree of odorant receptors (ORs).Included are ORs from M. cingulum (Mcin), M. mediator (Mmed), and N. vitripennis (Nvit).
Mentions: With exception of Orco, the predicted ORs shared quite low identity probably due to the high variance among OR gene family. Only three of 79 ORs (McinOR12, McinOR15, and McinOR42) showed more than 50% identity with known ORs in NCBI database (Table 4). The phylogenetic analysis also showed that ORs were extremely divergent between species but formed monophyletic group within same species (Fig. 4). However the highly conserved Orco shared 95–99% amino acid sequence identity and clustered in same branch with orthologous relation among three species (Fig. 4). All of the other McinORs were distributed in different branches of the phylogenetic tree. Eight species specific subgroups were identified consisting of different numbers of McinORs. The highest 14 McinORs (OR11, 24, 36, 52, 55, 56, 67, 68, 74, 78, 80, 81, 85, and 86) and the lowest three McinORs (OR30, 60 and 84) and another three McinORs (OR1, 7 and 59) clustered within the species specific subgroup. Others McinOR are clustered with N. vitripennis or M. mediator ORs.

Bottom Line: Insects are extremely successful animals whose odor perception is very prominent due to their sophisticated olfactory system.The sequences of all of these transcripts were confirmed by RT-PCR, and direct DNA sequencing.This reported result provides a comprehensive resource of the foundation in semiochemicals driven behaviors at molecular level in polyembryonic endoparasitoid.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory for the Biology of the Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.

ABSTRACT
Insects are extremely successful animals whose odor perception is very prominent due to their sophisticated olfactory system. The main chemosensory organ, antennae play a critical role in detecting odor in ambient environment before initiating appropriate behavioral responses. The antennal chemosensory receptor genes families have been suggested to be involved in olfactory signal transduction pathway as a sensory neuron response. The Macrocentrus cingulum is deployed successfully as a biological control agent for corn pest insects from the Lepidopteran genus Ostrinia. In this research, we assembled antennal transcriptomes of M. cingulum by using next generation sequencing to identify the major chemosensory receptors gene families. In total, 112 olfactory receptors candidates (79 odorant receptors, 20 gustatory receptors, and 13 ionotropic receptors) have been identified from the male and female antennal transcriptome. The sequences of all of these transcripts were confirmed by RT-PCR, and direct DNA sequencing. Expression profiles of gustatory receptors in olfactory and non-olfactory tissues were measured by RT-qPCR. The sex-specific and sex-biased chemoreceptors expression patterns suggested that they may have important functions in sense detection which behaviorally relevant to odor molecules. This reported result provides a comprehensive resource of the foundation in semiochemicals driven behaviors at molecular level in polyembryonic endoparasitoid.

No MeSH data available.


Related in: MedlinePlus