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Gene set of chemosensory receptors in the polyembryonic endoparasitoid Macrocentrus cingulum.

Ahmed T, Zhang T, Wang Z, He K, Bai S - Sci Rep (2016)

Bottom Line: Insects are extremely successful animals whose odor perception is very prominent due to their sophisticated olfactory system.The sequences of all of these transcripts were confirmed by RT-PCR, and direct DNA sequencing.This reported result provides a comprehensive resource of the foundation in semiochemicals driven behaviors at molecular level in polyembryonic endoparasitoid.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory for the Biology of the Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.

ABSTRACT
Insects are extremely successful animals whose odor perception is very prominent due to their sophisticated olfactory system. The main chemosensory organ, antennae play a critical role in detecting odor in ambient environment before initiating appropriate behavioral responses. The antennal chemosensory receptor genes families have been suggested to be involved in olfactory signal transduction pathway as a sensory neuron response. The Macrocentrus cingulum is deployed successfully as a biological control agent for corn pest insects from the Lepidopteran genus Ostrinia. In this research, we assembled antennal transcriptomes of M. cingulum by using next generation sequencing to identify the major chemosensory receptors gene families. In total, 112 olfactory receptors candidates (79 odorant receptors, 20 gustatory receptors, and 13 ionotropic receptors) have been identified from the male and female antennal transcriptome. The sequences of all of these transcripts were confirmed by RT-PCR, and direct DNA sequencing. Expression profiles of gustatory receptors in olfactory and non-olfactory tissues were measured by RT-qPCR. The sex-specific and sex-biased chemoreceptors expression patterns suggested that they may have important functions in sense detection which behaviorally relevant to odor molecules. This reported result provides a comprehensive resource of the foundation in semiochemicals driven behaviors at molecular level in polyembryonic endoparasitoid.

No MeSH data available.


Related in: MedlinePlus

Gene ontology (GO) annotation summary.GO analysis corresponding to 10,781 contig sequences in M. cingulum, as predicted for their involvement in (A) molecular function (level 2 GO categorization) and (B) biological process (level 2). For results presented as detailed bar diagrams, see supplementary Fig. S1.
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f2: Gene ontology (GO) annotation summary.GO analysis corresponding to 10,781 contig sequences in M. cingulum, as predicted for their involvement in (A) molecular function (level 2 GO categorization) and (B) biological process (level 2). For results presented as detailed bar diagrams, see supplementary Fig. S1.

Mentions: From Gene Ontology (GO) annotation the M. cingulum antennal transcriptome unigenes (10,781 of 41,254 unignes) were associated with GO terms which cover three domains: biological process, cellular component and molecular function (Fig. 2, and supplementary Fig. S1). In the terms of biological process; cellular, metabolic and single organism processes represented most of genes. In the molecular function terms, mostly associated with binding activity (e.g. nucleotide binding, odorant binding, ion binding), and catalytic activity (e.g. hydrolase and oxidoreductase activity). In the cellular category, cell, and cell part were the most abundant (Fig. 2).


Gene set of chemosensory receptors in the polyembryonic endoparasitoid Macrocentrus cingulum.

Ahmed T, Zhang T, Wang Z, He K, Bai S - Sci Rep (2016)

Gene ontology (GO) annotation summary.GO analysis corresponding to 10,781 contig sequences in M. cingulum, as predicted for their involvement in (A) molecular function (level 2 GO categorization) and (B) biological process (level 2). For results presented as detailed bar diagrams, see supplementary Fig. S1.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4835793&req=5

f2: Gene ontology (GO) annotation summary.GO analysis corresponding to 10,781 contig sequences in M. cingulum, as predicted for their involvement in (A) molecular function (level 2 GO categorization) and (B) biological process (level 2). For results presented as detailed bar diagrams, see supplementary Fig. S1.
Mentions: From Gene Ontology (GO) annotation the M. cingulum antennal transcriptome unigenes (10,781 of 41,254 unignes) were associated with GO terms which cover three domains: biological process, cellular component and molecular function (Fig. 2, and supplementary Fig. S1). In the terms of biological process; cellular, metabolic and single organism processes represented most of genes. In the molecular function terms, mostly associated with binding activity (e.g. nucleotide binding, odorant binding, ion binding), and catalytic activity (e.g. hydrolase and oxidoreductase activity). In the cellular category, cell, and cell part were the most abundant (Fig. 2).

Bottom Line: Insects are extremely successful animals whose odor perception is very prominent due to their sophisticated olfactory system.The sequences of all of these transcripts were confirmed by RT-PCR, and direct DNA sequencing.This reported result provides a comprehensive resource of the foundation in semiochemicals driven behaviors at molecular level in polyembryonic endoparasitoid.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory for the Biology of the Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.

ABSTRACT
Insects are extremely successful animals whose odor perception is very prominent due to their sophisticated olfactory system. The main chemosensory organ, antennae play a critical role in detecting odor in ambient environment before initiating appropriate behavioral responses. The antennal chemosensory receptor genes families have been suggested to be involved in olfactory signal transduction pathway as a sensory neuron response. The Macrocentrus cingulum is deployed successfully as a biological control agent for corn pest insects from the Lepidopteran genus Ostrinia. In this research, we assembled antennal transcriptomes of M. cingulum by using next generation sequencing to identify the major chemosensory receptors gene families. In total, 112 olfactory receptors candidates (79 odorant receptors, 20 gustatory receptors, and 13 ionotropic receptors) have been identified from the male and female antennal transcriptome. The sequences of all of these transcripts were confirmed by RT-PCR, and direct DNA sequencing. Expression profiles of gustatory receptors in olfactory and non-olfactory tissues were measured by RT-qPCR. The sex-specific and sex-biased chemoreceptors expression patterns suggested that they may have important functions in sense detection which behaviorally relevant to odor molecules. This reported result provides a comprehensive resource of the foundation in semiochemicals driven behaviors at molecular level in polyembryonic endoparasitoid.

No MeSH data available.


Related in: MedlinePlus