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Gene set of chemosensory receptors in the polyembryonic endoparasitoid Macrocentrus cingulum.

Ahmed T, Zhang T, Wang Z, He K, Bai S - Sci Rep (2016)

Bottom Line: Insects are extremely successful animals whose odor perception is very prominent due to their sophisticated olfactory system.The sequences of all of these transcripts were confirmed by RT-PCR, and direct DNA sequencing.This reported result provides a comprehensive resource of the foundation in semiochemicals driven behaviors at molecular level in polyembryonic endoparasitoid.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory for the Biology of the Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.

ABSTRACT
Insects are extremely successful animals whose odor perception is very prominent due to their sophisticated olfactory system. The main chemosensory organ, antennae play a critical role in detecting odor in ambient environment before initiating appropriate behavioral responses. The antennal chemosensory receptor genes families have been suggested to be involved in olfactory signal transduction pathway as a sensory neuron response. The Macrocentrus cingulum is deployed successfully as a biological control agent for corn pest insects from the Lepidopteran genus Ostrinia. In this research, we assembled antennal transcriptomes of M. cingulum by using next generation sequencing to identify the major chemosensory receptors gene families. In total, 112 olfactory receptors candidates (79 odorant receptors, 20 gustatory receptors, and 13 ionotropic receptors) have been identified from the male and female antennal transcriptome. The sequences of all of these transcripts were confirmed by RT-PCR, and direct DNA sequencing. Expression profiles of gustatory receptors in olfactory and non-olfactory tissues were measured by RT-qPCR. The sex-specific and sex-biased chemoreceptors expression patterns suggested that they may have important functions in sense detection which behaviorally relevant to odor molecules. This reported result provides a comprehensive resource of the foundation in semiochemicals driven behaviors at molecular level in polyembryonic endoparasitoid.

No MeSH data available.


Related in: MedlinePlus

Homology analyses results.The BLASTx annotations of M. cingulum antenna transcripts (A) E-value distribution, (B) similarity distribution, and (C) species distribution.
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f1: Homology analyses results.The BLASTx annotations of M. cingulum antenna transcripts (A) E-value distribution, (B) similarity distribution, and (C) species distribution.

Mentions: The functional annotations of the unigenes were performed mainly with deposited in diverse protein datasets listed in methods section. In total of 41,254 unigenes, 10,977 (26.6%) unigenes in the Nr database, 4,283 (10.38%) unigenes Nt data base, 5,248 (12.72%) in the KO database, 8,737 (21.17%) unigenes in the SwissPort database, 10,741 (26.03%) unigenes in the Pfam database, 10,781 (26.13%) unigenes in the GO database, 7,110 (17.23%) in the KOG database, 2,197 (5.32%) in the all database and 14,113 (34.21%) unigenes in the at least one database were annotated (Table 3). Using Nr database annotation, 7,114 (64.8%) unigenes matched to known proteins. Among the 10,977 annotated unigenes, 5,313 (48.4%) had a strong match (e-value smaller than1e−45) whereas 1,240 (11.3%) showed poor homology with e-value between 1e−15 and1e−5 (Fig. 1A). From the Nr annotation, 39.8% unigenes showed 60–80% and 37.8% unigenes showed 80–95% similarity with known proteins (Fig. 1B). Nr database queries revealed that 65.5% sequences closely matched to hymenopteran sequence (Microplitis demolitor 51.7%, Nasonia vitripennis 4.0%, Apis dorsata 2.8%, Cerapachys biroi 2.7 and Megachile rotundata 4.3%) (Fig. 1C).


Gene set of chemosensory receptors in the polyembryonic endoparasitoid Macrocentrus cingulum.

Ahmed T, Zhang T, Wang Z, He K, Bai S - Sci Rep (2016)

Homology analyses results.The BLASTx annotations of M. cingulum antenna transcripts (A) E-value distribution, (B) similarity distribution, and (C) species distribution.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4835793&req=5

f1: Homology analyses results.The BLASTx annotations of M. cingulum antenna transcripts (A) E-value distribution, (B) similarity distribution, and (C) species distribution.
Mentions: The functional annotations of the unigenes were performed mainly with deposited in diverse protein datasets listed in methods section. In total of 41,254 unigenes, 10,977 (26.6%) unigenes in the Nr database, 4,283 (10.38%) unigenes Nt data base, 5,248 (12.72%) in the KO database, 8,737 (21.17%) unigenes in the SwissPort database, 10,741 (26.03%) unigenes in the Pfam database, 10,781 (26.13%) unigenes in the GO database, 7,110 (17.23%) in the KOG database, 2,197 (5.32%) in the all database and 14,113 (34.21%) unigenes in the at least one database were annotated (Table 3). Using Nr database annotation, 7,114 (64.8%) unigenes matched to known proteins. Among the 10,977 annotated unigenes, 5,313 (48.4%) had a strong match (e-value smaller than1e−45) whereas 1,240 (11.3%) showed poor homology with e-value between 1e−15 and1e−5 (Fig. 1A). From the Nr annotation, 39.8% unigenes showed 60–80% and 37.8% unigenes showed 80–95% similarity with known proteins (Fig. 1B). Nr database queries revealed that 65.5% sequences closely matched to hymenopteran sequence (Microplitis demolitor 51.7%, Nasonia vitripennis 4.0%, Apis dorsata 2.8%, Cerapachys biroi 2.7 and Megachile rotundata 4.3%) (Fig. 1C).

Bottom Line: Insects are extremely successful animals whose odor perception is very prominent due to their sophisticated olfactory system.The sequences of all of these transcripts were confirmed by RT-PCR, and direct DNA sequencing.This reported result provides a comprehensive resource of the foundation in semiochemicals driven behaviors at molecular level in polyembryonic endoparasitoid.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory for the Biology of the Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.

ABSTRACT
Insects are extremely successful animals whose odor perception is very prominent due to their sophisticated olfactory system. The main chemosensory organ, antennae play a critical role in detecting odor in ambient environment before initiating appropriate behavioral responses. The antennal chemosensory receptor genes families have been suggested to be involved in olfactory signal transduction pathway as a sensory neuron response. The Macrocentrus cingulum is deployed successfully as a biological control agent for corn pest insects from the Lepidopteran genus Ostrinia. In this research, we assembled antennal transcriptomes of M. cingulum by using next generation sequencing to identify the major chemosensory receptors gene families. In total, 112 olfactory receptors candidates (79 odorant receptors, 20 gustatory receptors, and 13 ionotropic receptors) have been identified from the male and female antennal transcriptome. The sequences of all of these transcripts were confirmed by RT-PCR, and direct DNA sequencing. Expression profiles of gustatory receptors in olfactory and non-olfactory tissues were measured by RT-qPCR. The sex-specific and sex-biased chemoreceptors expression patterns suggested that they may have important functions in sense detection which behaviorally relevant to odor molecules. This reported result provides a comprehensive resource of the foundation in semiochemicals driven behaviors at molecular level in polyembryonic endoparasitoid.

No MeSH data available.


Related in: MedlinePlus