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The effects of Bt Cry1Ie toxin on bacterial diversity in the midgut of Apis mellifera ligustica (Hymenoptera: Apidae).

Jia HR, Geng LL, Li YH, Wang Q, Diao QY, Zhou T, Dai PL - Sci Rep (2016)

Bottom Line: The honey bee has been regarded as a key species in the environmental risk assessment of biotech crops.Newly emerged bees were fed with different concentrations of Cry1Ie toxin syrups (20 ng/mL, 200 ng/mL, and 20 μg/mL), pure sugar syrup, and 48 ppb of imidacloprid syrups, then sampled after 15 and 30 d.This work was the first to show the effects of Cry1Ie toxin on honey bees, and our study provided a theoretical basis for the biosafety assessment of transgenic Cry1Ie maize.

View Article: PubMed Central - PubMed

Affiliation: Ministry Key Laboratory of Pollinating Insect Biology, Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing 100093, China.

ABSTRACT
The honey bee has been regarded as a key species in the environmental risk assessment of biotech crops. Here, the potential adverse effects of Cry1Ie toxin on the midgut bacteria of the worker bees (Apis mellifera ligustica) were investigated under laboratory conditions. Newly emerged bees were fed with different concentrations of Cry1Ie toxin syrups (20 ng/mL, 200 ng/mL, and 20 μg/mL), pure sugar syrup, and 48 ppb of imidacloprid syrups, then sampled after 15 and 30 d. We characterized the dominant midgut bacteria and compared the composition and structure of the midgut bacterial community in all samples using the Illumina MiSeq platform targeting the V3-V4 regions of 16S rDNA. No significant differences in the diversity of the midgut bacteria were observed between the five treatments. This work was the first to show the effects of Cry1Ie toxin on honey bees, and our study provided a theoretical basis for the biosafety assessment of transgenic Cry1Ie maize.

No MeSH data available.


Related in: MedlinePlus

Differences in bacterial community diversity, richness and structures among five sample groups at 2 sampling time, respectively.Box plot of richness estimators of the gut microbiota of the five treatments at 2 sampling times. Each box plot represents each richness estimator. Significant differences are defined at the 95% confidence level.
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f5: Differences in bacterial community diversity, richness and structures among five sample groups at 2 sampling time, respectively.Box plot of richness estimators of the gut microbiota of the five treatments at 2 sampling times. Each box plot represents each richness estimator. Significant differences are defined at the 95% confidence level.

Mentions: Alpha diversity was applied to analysing the complexity of the species diversity for a sample. Then, the alpha diversity parameters of each sample, including community richness (ACE, Chao1), diversity indices (Shannon, Simpson), and sequencing depth (Good’s coverage) were determined with QIIME and displayed with R software (Table S5). A box plot of richness estimators was constructed using the QIIME toolkit (Fig. 5), providing a clear visualization of the relationships among the different sample groups. Alpha diversities were further tested by comparing the alpha diversity indexes between groups using one-way ANOVA (SPSS. 16.0), and no significant differences were observed across the five treatments in the same period (P > 0.05, Table S6).


The effects of Bt Cry1Ie toxin on bacterial diversity in the midgut of Apis mellifera ligustica (Hymenoptera: Apidae).

Jia HR, Geng LL, Li YH, Wang Q, Diao QY, Zhou T, Dai PL - Sci Rep (2016)

Differences in bacterial community diversity, richness and structures among five sample groups at 2 sampling time, respectively.Box plot of richness estimators of the gut microbiota of the five treatments at 2 sampling times. Each box plot represents each richness estimator. Significant differences are defined at the 95% confidence level.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4835784&req=5

f5: Differences in bacterial community diversity, richness and structures among five sample groups at 2 sampling time, respectively.Box plot of richness estimators of the gut microbiota of the five treatments at 2 sampling times. Each box plot represents each richness estimator. Significant differences are defined at the 95% confidence level.
Mentions: Alpha diversity was applied to analysing the complexity of the species diversity for a sample. Then, the alpha diversity parameters of each sample, including community richness (ACE, Chao1), diversity indices (Shannon, Simpson), and sequencing depth (Good’s coverage) were determined with QIIME and displayed with R software (Table S5). A box plot of richness estimators was constructed using the QIIME toolkit (Fig. 5), providing a clear visualization of the relationships among the different sample groups. Alpha diversities were further tested by comparing the alpha diversity indexes between groups using one-way ANOVA (SPSS. 16.0), and no significant differences were observed across the five treatments in the same period (P > 0.05, Table S6).

Bottom Line: The honey bee has been regarded as a key species in the environmental risk assessment of biotech crops.Newly emerged bees were fed with different concentrations of Cry1Ie toxin syrups (20 ng/mL, 200 ng/mL, and 20 μg/mL), pure sugar syrup, and 48 ppb of imidacloprid syrups, then sampled after 15 and 30 d.This work was the first to show the effects of Cry1Ie toxin on honey bees, and our study provided a theoretical basis for the biosafety assessment of transgenic Cry1Ie maize.

View Article: PubMed Central - PubMed

Affiliation: Ministry Key Laboratory of Pollinating Insect Biology, Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing 100093, China.

ABSTRACT
The honey bee has been regarded as a key species in the environmental risk assessment of biotech crops. Here, the potential adverse effects of Cry1Ie toxin on the midgut bacteria of the worker bees (Apis mellifera ligustica) were investigated under laboratory conditions. Newly emerged bees were fed with different concentrations of Cry1Ie toxin syrups (20 ng/mL, 200 ng/mL, and 20 μg/mL), pure sugar syrup, and 48 ppb of imidacloprid syrups, then sampled after 15 and 30 d. We characterized the dominant midgut bacteria and compared the composition and structure of the midgut bacterial community in all samples using the Illumina MiSeq platform targeting the V3-V4 regions of 16S rDNA. No significant differences in the diversity of the midgut bacteria were observed between the five treatments. This work was the first to show the effects of Cry1Ie toxin on honey bees, and our study provided a theoretical basis for the biosafety assessment of transgenic Cry1Ie maize.

No MeSH data available.


Related in: MedlinePlus