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The effects of Bt Cry1Ie toxin on bacterial diversity in the midgut of Apis mellifera ligustica (Hymenoptera: Apidae).

Jia HR, Geng LL, Li YH, Wang Q, Diao QY, Zhou T, Dai PL - Sci Rep (2016)

Bottom Line: The honey bee has been regarded as a key species in the environmental risk assessment of biotech crops.Newly emerged bees were fed with different concentrations of Cry1Ie toxin syrups (20 ng/mL, 200 ng/mL, and 20 μg/mL), pure sugar syrup, and 48 ppb of imidacloprid syrups, then sampled after 15 and 30 d.This work was the first to show the effects of Cry1Ie toxin on honey bees, and our study provided a theoretical basis for the biosafety assessment of transgenic Cry1Ie maize.

View Article: PubMed Central - PubMed

Affiliation: Ministry Key Laboratory of Pollinating Insect Biology, Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing 100093, China.

ABSTRACT
The honey bee has been regarded as a key species in the environmental risk assessment of biotech crops. Here, the potential adverse effects of Cry1Ie toxin on the midgut bacteria of the worker bees (Apis mellifera ligustica) were investigated under laboratory conditions. Newly emerged bees were fed with different concentrations of Cry1Ie toxin syrups (20 ng/mL, 200 ng/mL, and 20 μg/mL), pure sugar syrup, and 48 ppb of imidacloprid syrups, then sampled after 15 and 30 d. We characterized the dominant midgut bacteria and compared the composition and structure of the midgut bacterial community in all samples using the Illumina MiSeq platform targeting the V3-V4 regions of 16S rDNA. No significant differences in the diversity of the midgut bacteria were observed between the five treatments. This work was the first to show the effects of Cry1Ie toxin on honey bees, and our study provided a theoretical basis for the biosafety assessment of transgenic Cry1Ie maize.

No MeSH data available.


Related in: MedlinePlus

Relative abundance of the dominant midgut bacterial genera in the midgut of Apis mellifera ligustica adults across the five treatments at 2 sampling times.
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f3: Relative abundance of the dominant midgut bacterial genera in the midgut of Apis mellifera ligustica adults across the five treatments at 2 sampling times.

Mentions: The five feeding treatments were 20 ng/mL, 200 ng/mL, and 20 μg/mL of Cry1Ie toxin syrups, pure sugar syrup and 48 ppb of imidacloprid syrup. To visualize differences in the bacterial community, the histograms of the dominant midgut bacterial genera were constructed among the different sample groups using the QIIME toolkit (Fig. 3). For statistical analysis, the composition of dominant midgut bacterial genera among different groups was assessed using one-way ANOVA (SPSS. 16.0), and no significant effect was observed in the midgut bacterial community composition of adult honeybees fed on transgenic cry1Ah-maize pollen (P > 0.05, Table S4). The shifts of the bacterial community compositions were further corroborated by clear clustering of the dominant bacterial genus corresponding to different treatments in the heat map as shown in Fig. 4.


The effects of Bt Cry1Ie toxin on bacterial diversity in the midgut of Apis mellifera ligustica (Hymenoptera: Apidae).

Jia HR, Geng LL, Li YH, Wang Q, Diao QY, Zhou T, Dai PL - Sci Rep (2016)

Relative abundance of the dominant midgut bacterial genera in the midgut of Apis mellifera ligustica adults across the five treatments at 2 sampling times.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4835784&req=5

f3: Relative abundance of the dominant midgut bacterial genera in the midgut of Apis mellifera ligustica adults across the five treatments at 2 sampling times.
Mentions: The five feeding treatments were 20 ng/mL, 200 ng/mL, and 20 μg/mL of Cry1Ie toxin syrups, pure sugar syrup and 48 ppb of imidacloprid syrup. To visualize differences in the bacterial community, the histograms of the dominant midgut bacterial genera were constructed among the different sample groups using the QIIME toolkit (Fig. 3). For statistical analysis, the composition of dominant midgut bacterial genera among different groups was assessed using one-way ANOVA (SPSS. 16.0), and no significant effect was observed in the midgut bacterial community composition of adult honeybees fed on transgenic cry1Ah-maize pollen (P > 0.05, Table S4). The shifts of the bacterial community compositions were further corroborated by clear clustering of the dominant bacterial genus corresponding to different treatments in the heat map as shown in Fig. 4.

Bottom Line: The honey bee has been regarded as a key species in the environmental risk assessment of biotech crops.Newly emerged bees were fed with different concentrations of Cry1Ie toxin syrups (20 ng/mL, 200 ng/mL, and 20 μg/mL), pure sugar syrup, and 48 ppb of imidacloprid syrups, then sampled after 15 and 30 d.This work was the first to show the effects of Cry1Ie toxin on honey bees, and our study provided a theoretical basis for the biosafety assessment of transgenic Cry1Ie maize.

View Article: PubMed Central - PubMed

Affiliation: Ministry Key Laboratory of Pollinating Insect Biology, Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing 100093, China.

ABSTRACT
The honey bee has been regarded as a key species in the environmental risk assessment of biotech crops. Here, the potential adverse effects of Cry1Ie toxin on the midgut bacteria of the worker bees (Apis mellifera ligustica) were investigated under laboratory conditions. Newly emerged bees were fed with different concentrations of Cry1Ie toxin syrups (20 ng/mL, 200 ng/mL, and 20 μg/mL), pure sugar syrup, and 48 ppb of imidacloprid syrups, then sampled after 15 and 30 d. We characterized the dominant midgut bacteria and compared the composition and structure of the midgut bacterial community in all samples using the Illumina MiSeq platform targeting the V3-V4 regions of 16S rDNA. No significant differences in the diversity of the midgut bacteria were observed between the five treatments. This work was the first to show the effects of Cry1Ie toxin on honey bees, and our study provided a theoretical basis for the biosafety assessment of transgenic Cry1Ie maize.

No MeSH data available.


Related in: MedlinePlus