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The effects of Bt Cry1Ie toxin on bacterial diversity in the midgut of Apis mellifera ligustica (Hymenoptera: Apidae).

Jia HR, Geng LL, Li YH, Wang Q, Diao QY, Zhou T, Dai PL - Sci Rep (2016)

Bottom Line: The honey bee has been regarded as a key species in the environmental risk assessment of biotech crops.Newly emerged bees were fed with different concentrations of Cry1Ie toxin syrups (20 ng/mL, 200 ng/mL, and 20 μg/mL), pure sugar syrup, and 48 ppb of imidacloprid syrups, then sampled after 15 and 30 d.This work was the first to show the effects of Cry1Ie toxin on honey bees, and our study provided a theoretical basis for the biosafety assessment of transgenic Cry1Ie maize.

View Article: PubMed Central - PubMed

Affiliation: Ministry Key Laboratory of Pollinating Insect Biology, Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing 100093, China.

ABSTRACT
The honey bee has been regarded as a key species in the environmental risk assessment of biotech crops. Here, the potential adverse effects of Cry1Ie toxin on the midgut bacteria of the worker bees (Apis mellifera ligustica) were investigated under laboratory conditions. Newly emerged bees were fed with different concentrations of Cry1Ie toxin syrups (20 ng/mL, 200 ng/mL, and 20 μg/mL), pure sugar syrup, and 48 ppb of imidacloprid syrups, then sampled after 15 and 30 d. We characterized the dominant midgut bacteria and compared the composition and structure of the midgut bacterial community in all samples using the Illumina MiSeq platform targeting the V3-V4 regions of 16S rDNA. No significant differences in the diversity of the midgut bacteria were observed between the five treatments. This work was the first to show the effects of Cry1Ie toxin on honey bees, and our study provided a theoretical basis for the biosafety assessment of transgenic Cry1Ie maize.

No MeSH data available.


Related in: MedlinePlus

Relative abundance of the dominant bacterial in the midgut communities of Apis mellifera ligusticaat at genus level.Each bar represents the relative abundance of each sample. Each colour represents a particular bacterial family. Ck–Pure sugar syrup, T1–20 ng/mL of Cry1Ie toxin syrups, T2–200 ng/mL of Cry1Ie toxin syrups, T3–20 μg/mL of Cry1Ie toxin syrups; IMI–: 48 ppb of imidacloprid syrups.
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f2: Relative abundance of the dominant bacterial in the midgut communities of Apis mellifera ligusticaat at genus level.Each bar represents the relative abundance of each sample. Each colour represents a particular bacterial family. Ck–Pure sugar syrup, T1–20 ng/mL of Cry1Ie toxin syrups, T2–200 ng/mL of Cry1Ie toxin syrups, T3–20 μg/mL of Cry1Ie toxin syrups; IMI–: 48 ppb of imidacloprid syrups.

Mentions: Subsequently, all valid reads were classified into different taxonomy (phylum, class, order, family, and genera levels) using QIIME. The bacterial diversity and relative abundance of all samples in the different taxonomy are presented in Fig. S3. Six phylogenetic groups, α-, β- and γ-Proteobacteria, Firmicutes, Bacteroidetes and Actinobacteria were identified as the major bacterial taxa of the honeybee bacterial community in all samples. Based on relative abundance, Proteobacteria (mainly γ-proteobacteria) was the most abundant bacterial phylum in the honeybee midgut, with an average relative abundance of over 90%. Firmicutes and Actinobacteria had higher relative abundances compared with other phyla. The taxonomic distribution of each sample at the genus level is shown in Fig. 2. All core bacteria in A. Mellifera such as Gilliamella, Frischella, Snodgrassella, Lactobacillus and others, have been found in our study. Of all detected genera, Gilliamella of the Gamma proteobacteria was the most abundant genus.


The effects of Bt Cry1Ie toxin on bacterial diversity in the midgut of Apis mellifera ligustica (Hymenoptera: Apidae).

Jia HR, Geng LL, Li YH, Wang Q, Diao QY, Zhou T, Dai PL - Sci Rep (2016)

Relative abundance of the dominant bacterial in the midgut communities of Apis mellifera ligusticaat at genus level.Each bar represents the relative abundance of each sample. Each colour represents a particular bacterial family. Ck–Pure sugar syrup, T1–20 ng/mL of Cry1Ie toxin syrups, T2–200 ng/mL of Cry1Ie toxin syrups, T3–20 μg/mL of Cry1Ie toxin syrups; IMI–: 48 ppb of imidacloprid syrups.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4835784&req=5

f2: Relative abundance of the dominant bacterial in the midgut communities of Apis mellifera ligusticaat at genus level.Each bar represents the relative abundance of each sample. Each colour represents a particular bacterial family. Ck–Pure sugar syrup, T1–20 ng/mL of Cry1Ie toxin syrups, T2–200 ng/mL of Cry1Ie toxin syrups, T3–20 μg/mL of Cry1Ie toxin syrups; IMI–: 48 ppb of imidacloprid syrups.
Mentions: Subsequently, all valid reads were classified into different taxonomy (phylum, class, order, family, and genera levels) using QIIME. The bacterial diversity and relative abundance of all samples in the different taxonomy are presented in Fig. S3. Six phylogenetic groups, α-, β- and γ-Proteobacteria, Firmicutes, Bacteroidetes and Actinobacteria were identified as the major bacterial taxa of the honeybee bacterial community in all samples. Based on relative abundance, Proteobacteria (mainly γ-proteobacteria) was the most abundant bacterial phylum in the honeybee midgut, with an average relative abundance of over 90%. Firmicutes and Actinobacteria had higher relative abundances compared with other phyla. The taxonomic distribution of each sample at the genus level is shown in Fig. 2. All core bacteria in A. Mellifera such as Gilliamella, Frischella, Snodgrassella, Lactobacillus and others, have been found in our study. Of all detected genera, Gilliamella of the Gamma proteobacteria was the most abundant genus.

Bottom Line: The honey bee has been regarded as a key species in the environmental risk assessment of biotech crops.Newly emerged bees were fed with different concentrations of Cry1Ie toxin syrups (20 ng/mL, 200 ng/mL, and 20 μg/mL), pure sugar syrup, and 48 ppb of imidacloprid syrups, then sampled after 15 and 30 d.This work was the first to show the effects of Cry1Ie toxin on honey bees, and our study provided a theoretical basis for the biosafety assessment of transgenic Cry1Ie maize.

View Article: PubMed Central - PubMed

Affiliation: Ministry Key Laboratory of Pollinating Insect Biology, Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing 100093, China.

ABSTRACT
The honey bee has been regarded as a key species in the environmental risk assessment of biotech crops. Here, the potential adverse effects of Cry1Ie toxin on the midgut bacteria of the worker bees (Apis mellifera ligustica) were investigated under laboratory conditions. Newly emerged bees were fed with different concentrations of Cry1Ie toxin syrups (20 ng/mL, 200 ng/mL, and 20 μg/mL), pure sugar syrup, and 48 ppb of imidacloprid syrups, then sampled after 15 and 30 d. We characterized the dominant midgut bacteria and compared the composition and structure of the midgut bacterial community in all samples using the Illumina MiSeq platform targeting the V3-V4 regions of 16S rDNA. No significant differences in the diversity of the midgut bacteria were observed between the five treatments. This work was the first to show the effects of Cry1Ie toxin on honey bees, and our study provided a theoretical basis for the biosafety assessment of transgenic Cry1Ie maize.

No MeSH data available.


Related in: MedlinePlus