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The effects of Bt Cry1Ie toxin on bacterial diversity in the midgut of Apis mellifera ligustica (Hymenoptera: Apidae).

Jia HR, Geng LL, Li YH, Wang Q, Diao QY, Zhou T, Dai PL - Sci Rep (2016)

Bottom Line: The honey bee has been regarded as a key species in the environmental risk assessment of biotech crops.Newly emerged bees were fed with different concentrations of Cry1Ie toxin syrups (20 ng/mL, 200 ng/mL, and 20 μg/mL), pure sugar syrup, and 48 ppb of imidacloprid syrups, then sampled after 15 and 30 d.This work was the first to show the effects of Cry1Ie toxin on honey bees, and our study provided a theoretical basis for the biosafety assessment of transgenic Cry1Ie maize.

View Article: PubMed Central - PubMed

Affiliation: Ministry Key Laboratory of Pollinating Insect Biology, Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing 100093, China.

ABSTRACT
The honey bee has been regarded as a key species in the environmental risk assessment of biotech crops. Here, the potential adverse effects of Cry1Ie toxin on the midgut bacteria of the worker bees (Apis mellifera ligustica) were investigated under laboratory conditions. Newly emerged bees were fed with different concentrations of Cry1Ie toxin syrups (20 ng/mL, 200 ng/mL, and 20 μg/mL), pure sugar syrup, and 48 ppb of imidacloprid syrups, then sampled after 15 and 30 d. We characterized the dominant midgut bacteria and compared the composition and structure of the midgut bacterial community in all samples using the Illumina MiSeq platform targeting the V3-V4 regions of 16S rDNA. No significant differences in the diversity of the midgut bacteria were observed between the five treatments. This work was the first to show the effects of Cry1Ie toxin on honey bees, and our study provided a theoretical basis for the biosafety assessment of transgenic Cry1Ie maize.

No MeSH data available.


Related in: MedlinePlus

Log-transformed abundance of the 16S rRNA gene across different treatments at 2 sampling times.
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f1: Log-transformed abundance of the 16S rRNA gene across different treatments at 2 sampling times.

Mentions: As shown in Fig. S1, a standard curve (y = −0.362x + 10.319, R2 = 0.995) was established by plotting the threshold cycle (Ct) value against the logarithm of the 16 S rRNA gene initial copy number for each dilution. The 16S rRNA gene copy number in each sample was calculated by a linear regression of the normalized sample Ct values to the standard curve (Table S1). The log-transformed abundance of the copy numbers across different treatments are shown in Fig. 1. For further analyses by one-way ANOVA, no significant differences were observed among different treatments (P > 0.05, Table S2). This result showed that Cry1Ah toxin had no effect on the abundance of the bacterial 16S ribosomal RNA (rRNA) gene.


The effects of Bt Cry1Ie toxin on bacterial diversity in the midgut of Apis mellifera ligustica (Hymenoptera: Apidae).

Jia HR, Geng LL, Li YH, Wang Q, Diao QY, Zhou T, Dai PL - Sci Rep (2016)

Log-transformed abundance of the 16S rRNA gene across different treatments at 2 sampling times.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4835784&req=5

f1: Log-transformed abundance of the 16S rRNA gene across different treatments at 2 sampling times.
Mentions: As shown in Fig. S1, a standard curve (y = −0.362x + 10.319, R2 = 0.995) was established by plotting the threshold cycle (Ct) value against the logarithm of the 16 S rRNA gene initial copy number for each dilution. The 16S rRNA gene copy number in each sample was calculated by a linear regression of the normalized sample Ct values to the standard curve (Table S1). The log-transformed abundance of the copy numbers across different treatments are shown in Fig. 1. For further analyses by one-way ANOVA, no significant differences were observed among different treatments (P > 0.05, Table S2). This result showed that Cry1Ah toxin had no effect on the abundance of the bacterial 16S ribosomal RNA (rRNA) gene.

Bottom Line: The honey bee has been regarded as a key species in the environmental risk assessment of biotech crops.Newly emerged bees were fed with different concentrations of Cry1Ie toxin syrups (20 ng/mL, 200 ng/mL, and 20 μg/mL), pure sugar syrup, and 48 ppb of imidacloprid syrups, then sampled after 15 and 30 d.This work was the first to show the effects of Cry1Ie toxin on honey bees, and our study provided a theoretical basis for the biosafety assessment of transgenic Cry1Ie maize.

View Article: PubMed Central - PubMed

Affiliation: Ministry Key Laboratory of Pollinating Insect Biology, Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing 100093, China.

ABSTRACT
The honey bee has been regarded as a key species in the environmental risk assessment of biotech crops. Here, the potential adverse effects of Cry1Ie toxin on the midgut bacteria of the worker bees (Apis mellifera ligustica) were investigated under laboratory conditions. Newly emerged bees were fed with different concentrations of Cry1Ie toxin syrups (20 ng/mL, 200 ng/mL, and 20 μg/mL), pure sugar syrup, and 48 ppb of imidacloprid syrups, then sampled after 15 and 30 d. We characterized the dominant midgut bacteria and compared the composition and structure of the midgut bacterial community in all samples using the Illumina MiSeq platform targeting the V3-V4 regions of 16S rDNA. No significant differences in the diversity of the midgut bacteria were observed between the five treatments. This work was the first to show the effects of Cry1Ie toxin on honey bees, and our study provided a theoretical basis for the biosafety assessment of transgenic Cry1Ie maize.

No MeSH data available.


Related in: MedlinePlus