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Somatic embryogenesis and enhanced shoot organogenesis in Metabriggsia ovalifolia W. T. Wang.

Ouyang Y, Chen Y, Lü J, Teixeira da Silva JA, Zhang X, Ma G - Sci Rep (2016)

Bottom Line: Thus, somatic embryogenesis in this plant needs a high cytokinin concentration (BA; TDZ), as evidenced by histology.Somatic embryos germinated easily when left on the same media, but formed adventitious roots in two weeks on MS supplemented with 0.5 μM NAA, 0.5 μM IBA and 0.1% activated charcoal.Over 93% of plantlets survived following acclimatization and transfer to a mixture of sand and vermiculite (1:1, v/v) in trays.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, the Chinese Academy of Sciences, Guangzhou, 510650, China.

ABSTRACT
An efficient protocol providing a dual regeneration pathway via direct shoot organogenesis and somatic embryogenesis for an endangered species, Metabriggsia ovalifolia W. T. Wang, was established from leaf explants. When applied at 2.5 μM, the cytokinins 6-benzyladenine (BA) or thidiazuron (TDZ) and the auxins indole-3-butyric acid (IBA), α-naphthaleneacetic acid (NAA) and indole-3-acetic acid (IAA) could induce shoots when on basal Murashige and Skoog (MS) medium. BA and TDZ could induce more adventitious shoots (19.1 and 31.2/explant, respectively) than NAA (4.6/explant), IBA (5.7/explant) or IAA (6.4/explant). BA and TDZ at 5-10 μM could induce both shoots and somatic embryos. A higher concentration of TDZ (25 μM) induced only somatic embryos (39.8/explant). The same concentration of BA induced both adventitious shoots (23.6/explant) and somatic embryos (9.7/explant). Thus, somatic embryogenesis in this plant needs a high cytokinin concentration (BA; TDZ), as evidenced by histology. Somatic embryos germinated easily when left on the same media, but formed adventitious roots in two weeks on MS supplemented with 0.5 μM NAA, 0.5 μM IBA and 0.1% activated charcoal. Over 93% of plantlets survived following acclimatization and transfer to a mixture of sand and vermiculite (1:1, v/v) in trays.

No MeSH data available.


Plant acclimatization, transplantation and the detection of a mutant flowering plant of Metabriggsia ovalifolia.(a) Two-month-old plants. (b) Six-month-old plants. Bars = 2 cm.
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f5: Plant acclimatization, transplantation and the detection of a mutant flowering plant of Metabriggsia ovalifolia.(a) Two-month-old plants. (b) Six-month-old plants. Bars = 2 cm.

Mentions: Clusters of shoots were divided into individual shoots and transferred to rooting media and rooting was most rapid when exposed to 20 days of continuous light. After 50 days, plantlets with a well-established root system were transferred to a substrate of sand and vermiculite (1:1) leading to more than 95% survival without any obvious morphological variation. Root formation was induced within two weeks as the somatic embryos with an epicotyl were transferred to the rooting medium supplemented with 0.5 μM NAA, 0.5 μM IBA and 0.1% activated charcoal. After plantlets were cultured for another four weeks in rooting medium, they grew to 4–5 cm in height with 4–6 leaves (Fig. 5a). When plantlets were transferred to a sand and vermiculite substrate, more than 93% of the plantlets survived, all but one showing a normal (wild type) phenotypic appearance (Fig. 4a,b).


Somatic embryogenesis and enhanced shoot organogenesis in Metabriggsia ovalifolia W. T. Wang.

Ouyang Y, Chen Y, Lü J, Teixeira da Silva JA, Zhang X, Ma G - Sci Rep (2016)

Plant acclimatization, transplantation and the detection of a mutant flowering plant of Metabriggsia ovalifolia.(a) Two-month-old plants. (b) Six-month-old plants. Bars = 2 cm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4835782&req=5

f5: Plant acclimatization, transplantation and the detection of a mutant flowering plant of Metabriggsia ovalifolia.(a) Two-month-old plants. (b) Six-month-old plants. Bars = 2 cm.
Mentions: Clusters of shoots were divided into individual shoots and transferred to rooting media and rooting was most rapid when exposed to 20 days of continuous light. After 50 days, plantlets with a well-established root system were transferred to a substrate of sand and vermiculite (1:1) leading to more than 95% survival without any obvious morphological variation. Root formation was induced within two weeks as the somatic embryos with an epicotyl were transferred to the rooting medium supplemented with 0.5 μM NAA, 0.5 μM IBA and 0.1% activated charcoal. After plantlets were cultured for another four weeks in rooting medium, they grew to 4–5 cm in height with 4–6 leaves (Fig. 5a). When plantlets were transferred to a sand and vermiculite substrate, more than 93% of the plantlets survived, all but one showing a normal (wild type) phenotypic appearance (Fig. 4a,b).

Bottom Line: Thus, somatic embryogenesis in this plant needs a high cytokinin concentration (BA; TDZ), as evidenced by histology.Somatic embryos germinated easily when left on the same media, but formed adventitious roots in two weeks on MS supplemented with 0.5 μM NAA, 0.5 μM IBA and 0.1% activated charcoal.Over 93% of plantlets survived following acclimatization and transfer to a mixture of sand and vermiculite (1:1, v/v) in trays.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, the Chinese Academy of Sciences, Guangzhou, 510650, China.

ABSTRACT
An efficient protocol providing a dual regeneration pathway via direct shoot organogenesis and somatic embryogenesis for an endangered species, Metabriggsia ovalifolia W. T. Wang, was established from leaf explants. When applied at 2.5 μM, the cytokinins 6-benzyladenine (BA) or thidiazuron (TDZ) and the auxins indole-3-butyric acid (IBA), α-naphthaleneacetic acid (NAA) and indole-3-acetic acid (IAA) could induce shoots when on basal Murashige and Skoog (MS) medium. BA and TDZ could induce more adventitious shoots (19.1 and 31.2/explant, respectively) than NAA (4.6/explant), IBA (5.7/explant) or IAA (6.4/explant). BA and TDZ at 5-10 μM could induce both shoots and somatic embryos. A higher concentration of TDZ (25 μM) induced only somatic embryos (39.8/explant). The same concentration of BA induced both adventitious shoots (23.6/explant) and somatic embryos (9.7/explant). Thus, somatic embryogenesis in this plant needs a high cytokinin concentration (BA; TDZ), as evidenced by histology. Somatic embryos germinated easily when left on the same media, but formed adventitious roots in two weeks on MS supplemented with 0.5 μM NAA, 0.5 μM IBA and 0.1% activated charcoal. Over 93% of plantlets survived following acclimatization and transfer to a mixture of sand and vermiculite (1:1, v/v) in trays.

No MeSH data available.