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Single-step cycle pulse operation of the label-free electrochemiluminescence immunosensor based on branched polypyrrole for carcinoembryonic antigen detection.

Zhu W, Wang Q, Ma H, Lv X, Wu D, Sun X, Du B, Wei Q - Sci Rep (2016)

Bottom Line: Moreover, 1-butylpyridinium tetrafluroborate ([BPy]BF4) were used to disperse luminol functional-Au NPs@polypyrrole nanocomposites, resulting in the film-formation of composites on the electrode, which could improve the stability of immunosensor.The proposed method presents good ECL response for the detection of CEA allowing a wide linear range from 0.01 pg/mL to 10 ng/mL and a limit of detection as low as 3 fg/mL.The immunosensor would be a promising tool in the early diagnosis of CEA due to its high sensitivity, simplicity and cost-effective.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Chemical Sensing &Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, P.R. China.

ABSTRACT
A novel label-free electrochemiluminescence (ECL) immunosensor based on luminol functional-Au NPs@polypyrrole has been developed for the detection of carcinoembryonic antigen (CEA). In this work, polypyrrole prepared by chemical polymerization provided a large surface area to load amounts of gold nanoparticles (Au NPs). Au NPs could not only attach abundant luminol for the enhancement of ECL signal, but also provide a friendly microenvironment for the immobilization of antibodies. Moreover, 1-butylpyridinium tetrafluroborate ([BPy]BF4) were used to disperse luminol functional-Au NPs@polypyrrole nanocomposites, resulting in the film-formation of composites on the electrode, which could improve the stability of immunosensor. In particular, employment of single-step cycle pulse could limit the consecutive reaction between luminol and H2O2 efficiently, thus leading to stable and strong signals. The proposed method presents good ECL response for the detection of CEA allowing a wide linear range from 0.01 pg/mL to 10 ng/mL and a limit of detection as low as 3 fg/mL. The immunosensor would be a promising tool in the early diagnosis of CEA due to its high sensitivity, simplicity and cost-effective.

No MeSH data available.


(A) Calibration curve of the immunosensor for different concentrations of CEA measured in CBS (pH 10.4) containing 25 mM H2O2 (insert is the ECL response of the immunosensor to different concentrations of CEA, from left to right: 0.00001, 0.00005, 0.0001, 0.0005, 0.001, 0.005, 0.01, 0.05, 0.1, 0.5, 1, 5 and 10 ng/mL). (B) The repeatability of the proposed ECL modified electrodes with 1 ng/mL CEA. (C) ECL intensity of the ECL sensor to 0.1 ng/mL CEA (1), 0.1 ng/mL CEA + 10 ng/mL BSA (2), 0.1 ng/mL CEA + 10 ng/mL glucose (3), 0.1 ng/mL CEA + 10 ng/ mL α-Fetoprotein (4), Error bar = SD (n = 3). (D) The stability of ECL modified electrode with 0.1 ng/mL CEA measured in CBS (pH 10.4) containing 25 mM H2O2 under single-step cycle pulse.
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f6: (A) Calibration curve of the immunosensor for different concentrations of CEA measured in CBS (pH 10.4) containing 25 mM H2O2 (insert is the ECL response of the immunosensor to different concentrations of CEA, from left to right: 0.00001, 0.00005, 0.0001, 0.0005, 0.001, 0.005, 0.01, 0.05, 0.1, 0.5, 1, 5 and 10 ng/mL). (B) The repeatability of the proposed ECL modified electrodes with 1 ng/mL CEA. (C) ECL intensity of the ECL sensor to 0.1 ng/mL CEA (1), 0.1 ng/mL CEA + 10 ng/mL BSA (2), 0.1 ng/mL CEA + 10 ng/mL glucose (3), 0.1 ng/mL CEA + 10 ng/ mL α-Fetoprotein (4), Error bar = SD (n = 3). (D) The stability of ECL modified electrode with 0.1 ng/mL CEA measured in CBS (pH 10.4) containing 25 mM H2O2 under single-step cycle pulse.

Mentions: Under the optimized experimental conditions, the developed label-free immunosensor was applied to quantify the target CEA from 0.01 pg/mL to 10 ng/mL. As seen from Fig. 6A, a linear proportion between the ECL intensity and the logarithmic values of the CEA concentration could be achieved in the dynamic range from 0.01 pg/mL to 10 ng/mL. The linear equation was I = 6293.25 − 1854.14 lg (c, ng/mL) with a correlation coefficient of 0.9945. Compared with limit of detection (LOD) for amperometry (60 pg/mL)8, voltammetry (5 pg/mL)37, potentiometry (500 pg/mL)38, capillary electrophoresis (4.8 pg/mL)9, fluorometry (5 pg/mL)39, electrochemistry (2.36 pg/mL)11 (Table S1), the proposed method exhibited a lower LOD (3 fg/mL, signal/noise [S/N] ratio = 3). This is probably due to the enhancement of Au NPs and the excellent conductivity of Au NPs@PPy nanocomposites. Based on the above facts, it could be speculated that the designed immunoassay was effective for CEA detection.


Single-step cycle pulse operation of the label-free electrochemiluminescence immunosensor based on branched polypyrrole for carcinoembryonic antigen detection.

Zhu W, Wang Q, Ma H, Lv X, Wu D, Sun X, Du B, Wei Q - Sci Rep (2016)

(A) Calibration curve of the immunosensor for different concentrations of CEA measured in CBS (pH 10.4) containing 25 mM H2O2 (insert is the ECL response of the immunosensor to different concentrations of CEA, from left to right: 0.00001, 0.00005, 0.0001, 0.0005, 0.001, 0.005, 0.01, 0.05, 0.1, 0.5, 1, 5 and 10 ng/mL). (B) The repeatability of the proposed ECL modified electrodes with 1 ng/mL CEA. (C) ECL intensity of the ECL sensor to 0.1 ng/mL CEA (1), 0.1 ng/mL CEA + 10 ng/mL BSA (2), 0.1 ng/mL CEA + 10 ng/mL glucose (3), 0.1 ng/mL CEA + 10 ng/ mL α-Fetoprotein (4), Error bar = SD (n = 3). (D) The stability of ECL modified electrode with 0.1 ng/mL CEA measured in CBS (pH 10.4) containing 25 mM H2O2 under single-step cycle pulse.
© Copyright Policy - open-access
Related In: Results  -  Collection

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f6: (A) Calibration curve of the immunosensor for different concentrations of CEA measured in CBS (pH 10.4) containing 25 mM H2O2 (insert is the ECL response of the immunosensor to different concentrations of CEA, from left to right: 0.00001, 0.00005, 0.0001, 0.0005, 0.001, 0.005, 0.01, 0.05, 0.1, 0.5, 1, 5 and 10 ng/mL). (B) The repeatability of the proposed ECL modified electrodes with 1 ng/mL CEA. (C) ECL intensity of the ECL sensor to 0.1 ng/mL CEA (1), 0.1 ng/mL CEA + 10 ng/mL BSA (2), 0.1 ng/mL CEA + 10 ng/mL glucose (3), 0.1 ng/mL CEA + 10 ng/ mL α-Fetoprotein (4), Error bar = SD (n = 3). (D) The stability of ECL modified electrode with 0.1 ng/mL CEA measured in CBS (pH 10.4) containing 25 mM H2O2 under single-step cycle pulse.
Mentions: Under the optimized experimental conditions, the developed label-free immunosensor was applied to quantify the target CEA from 0.01 pg/mL to 10 ng/mL. As seen from Fig. 6A, a linear proportion between the ECL intensity and the logarithmic values of the CEA concentration could be achieved in the dynamic range from 0.01 pg/mL to 10 ng/mL. The linear equation was I = 6293.25 − 1854.14 lg (c, ng/mL) with a correlation coefficient of 0.9945. Compared with limit of detection (LOD) for amperometry (60 pg/mL)8, voltammetry (5 pg/mL)37, potentiometry (500 pg/mL)38, capillary electrophoresis (4.8 pg/mL)9, fluorometry (5 pg/mL)39, electrochemistry (2.36 pg/mL)11 (Table S1), the proposed method exhibited a lower LOD (3 fg/mL, signal/noise [S/N] ratio = 3). This is probably due to the enhancement of Au NPs and the excellent conductivity of Au NPs@PPy nanocomposites. Based on the above facts, it could be speculated that the designed immunoassay was effective for CEA detection.

Bottom Line: Moreover, 1-butylpyridinium tetrafluroborate ([BPy]BF4) were used to disperse luminol functional-Au NPs@polypyrrole nanocomposites, resulting in the film-formation of composites on the electrode, which could improve the stability of immunosensor.The proposed method presents good ECL response for the detection of CEA allowing a wide linear range from 0.01 pg/mL to 10 ng/mL and a limit of detection as low as 3 fg/mL.The immunosensor would be a promising tool in the early diagnosis of CEA due to its high sensitivity, simplicity and cost-effective.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Chemical Sensing &Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, P.R. China.

ABSTRACT
A novel label-free electrochemiluminescence (ECL) immunosensor based on luminol functional-Au NPs@polypyrrole has been developed for the detection of carcinoembryonic antigen (CEA). In this work, polypyrrole prepared by chemical polymerization provided a large surface area to load amounts of gold nanoparticles (Au NPs). Au NPs could not only attach abundant luminol for the enhancement of ECL signal, but also provide a friendly microenvironment for the immobilization of antibodies. Moreover, 1-butylpyridinium tetrafluroborate ([BPy]BF4) were used to disperse luminol functional-Au NPs@polypyrrole nanocomposites, resulting in the film-formation of composites on the electrode, which could improve the stability of immunosensor. In particular, employment of single-step cycle pulse could limit the consecutive reaction between luminol and H2O2 efficiently, thus leading to stable and strong signals. The proposed method presents good ECL response for the detection of CEA allowing a wide linear range from 0.01 pg/mL to 10 ng/mL and a limit of detection as low as 3 fg/mL. The immunosensor would be a promising tool in the early diagnosis of CEA due to its high sensitivity, simplicity and cost-effective.

No MeSH data available.