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Single-step cycle pulse operation of the label-free electrochemiluminescence immunosensor based on branched polypyrrole for carcinoembryonic antigen detection.

Zhu W, Wang Q, Ma H, Lv X, Wu D, Sun X, Du B, Wei Q - Sci Rep (2016)

Bottom Line: Moreover, 1-butylpyridinium tetrafluroborate ([BPy]BF4) were used to disperse luminol functional-Au NPs@polypyrrole nanocomposites, resulting in the film-formation of composites on the electrode, which could improve the stability of immunosensor.The proposed method presents good ECL response for the detection of CEA allowing a wide linear range from 0.01 pg/mL to 10 ng/mL and a limit of detection as low as 3 fg/mL.The immunosensor would be a promising tool in the early diagnosis of CEA due to its high sensitivity, simplicity and cost-effective.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Chemical Sensing &Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, P.R. China.

ABSTRACT
A novel label-free electrochemiluminescence (ECL) immunosensor based on luminol functional-Au NPs@polypyrrole has been developed for the detection of carcinoembryonic antigen (CEA). In this work, polypyrrole prepared by chemical polymerization provided a large surface area to load amounts of gold nanoparticles (Au NPs). Au NPs could not only attach abundant luminol for the enhancement of ECL signal, but also provide a friendly microenvironment for the immobilization of antibodies. Moreover, 1-butylpyridinium tetrafluroborate ([BPy]BF4) were used to disperse luminol functional-Au NPs@polypyrrole nanocomposites, resulting in the film-formation of composites on the electrode, which could improve the stability of immunosensor. In particular, employment of single-step cycle pulse could limit the consecutive reaction between luminol and H2O2 efficiently, thus leading to stable and strong signals. The proposed method presents good ECL response for the detection of CEA allowing a wide linear range from 0.01 pg/mL to 10 ng/mL and a limit of detection as low as 3 fg/mL. The immunosensor would be a promising tool in the early diagnosis of CEA due to its high sensitivity, simplicity and cost-effective.

No MeSH data available.


The effect of pH (A) and concentration of H2O2 (B). Error bar = SD (n = 3).
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f5: The effect of pH (A) and concentration of H2O2 (B). Error bar = SD (n = 3).

Mentions: As is well-known, the ECL intensity of the luminol-H2O2 system is pH-dependent and generally performs better in alkaline media3435. Therefore, the effect of pH value was firstly investigated over the range of 9.8–10.8. As shown in Fig. 5A, the maximum enhancement of ECL intensity was observed at pH 10.4. The reason might be as follows: when pH value increased from 9.8 to 10.4, the oxidation strength of H2O2 was enhanced and the ECL intensity increased gradually. After pH value above 10.4, highly alkaline surrounding facilitated the decomposition of H2O2 leading to the formation of bubbles36. So that pH 10.4 was selected as the optimal condition.


Single-step cycle pulse operation of the label-free electrochemiluminescence immunosensor based on branched polypyrrole for carcinoembryonic antigen detection.

Zhu W, Wang Q, Ma H, Lv X, Wu D, Sun X, Du B, Wei Q - Sci Rep (2016)

The effect of pH (A) and concentration of H2O2 (B). Error bar = SD (n = 3).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4835776&req=5

f5: The effect of pH (A) and concentration of H2O2 (B). Error bar = SD (n = 3).
Mentions: As is well-known, the ECL intensity of the luminol-H2O2 system is pH-dependent and generally performs better in alkaline media3435. Therefore, the effect of pH value was firstly investigated over the range of 9.8–10.8. As shown in Fig. 5A, the maximum enhancement of ECL intensity was observed at pH 10.4. The reason might be as follows: when pH value increased from 9.8 to 10.4, the oxidation strength of H2O2 was enhanced and the ECL intensity increased gradually. After pH value above 10.4, highly alkaline surrounding facilitated the decomposition of H2O2 leading to the formation of bubbles36. So that pH 10.4 was selected as the optimal condition.

Bottom Line: Moreover, 1-butylpyridinium tetrafluroborate ([BPy]BF4) were used to disperse luminol functional-Au NPs@polypyrrole nanocomposites, resulting in the film-formation of composites on the electrode, which could improve the stability of immunosensor.The proposed method presents good ECL response for the detection of CEA allowing a wide linear range from 0.01 pg/mL to 10 ng/mL and a limit of detection as low as 3 fg/mL.The immunosensor would be a promising tool in the early diagnosis of CEA due to its high sensitivity, simplicity and cost-effective.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Chemical Sensing &Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, P.R. China.

ABSTRACT
A novel label-free electrochemiluminescence (ECL) immunosensor based on luminol functional-Au NPs@polypyrrole has been developed for the detection of carcinoembryonic antigen (CEA). In this work, polypyrrole prepared by chemical polymerization provided a large surface area to load amounts of gold nanoparticles (Au NPs). Au NPs could not only attach abundant luminol for the enhancement of ECL signal, but also provide a friendly microenvironment for the immobilization of antibodies. Moreover, 1-butylpyridinium tetrafluroborate ([BPy]BF4) were used to disperse luminol functional-Au NPs@polypyrrole nanocomposites, resulting in the film-formation of composites on the electrode, which could improve the stability of immunosensor. In particular, employment of single-step cycle pulse could limit the consecutive reaction between luminol and H2O2 efficiently, thus leading to stable and strong signals. The proposed method presents good ECL response for the detection of CEA allowing a wide linear range from 0.01 pg/mL to 10 ng/mL and a limit of detection as low as 3 fg/mL. The immunosensor would be a promising tool in the early diagnosis of CEA due to its high sensitivity, simplicity and cost-effective.

No MeSH data available.