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Single-step cycle pulse operation of the label-free electrochemiluminescence immunosensor based on branched polypyrrole for carcinoembryonic antigen detection.

Zhu W, Wang Q, Ma H, Lv X, Wu D, Sun X, Du B, Wei Q - Sci Rep (2016)

Bottom Line: Moreover, 1-butylpyridinium tetrafluroborate ([BPy]BF4) were used to disperse luminol functional-Au NPs@polypyrrole nanocomposites, resulting in the film-formation of composites on the electrode, which could improve the stability of immunosensor.The proposed method presents good ECL response for the detection of CEA allowing a wide linear range from 0.01 pg/mL to 10 ng/mL and a limit of detection as low as 3 fg/mL.The immunosensor would be a promising tool in the early diagnosis of CEA due to its high sensitivity, simplicity and cost-effective.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Chemical Sensing &Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, P.R. China.

ABSTRACT
A novel label-free electrochemiluminescence (ECL) immunosensor based on luminol functional-Au NPs@polypyrrole has been developed for the detection of carcinoembryonic antigen (CEA). In this work, polypyrrole prepared by chemical polymerization provided a large surface area to load amounts of gold nanoparticles (Au NPs). Au NPs could not only attach abundant luminol for the enhancement of ECL signal, but also provide a friendly microenvironment for the immobilization of antibodies. Moreover, 1-butylpyridinium tetrafluroborate ([BPy]BF4) were used to disperse luminol functional-Au NPs@polypyrrole nanocomposites, resulting in the film-formation of composites on the electrode, which could improve the stability of immunosensor. In particular, employment of single-step cycle pulse could limit the consecutive reaction between luminol and H2O2 efficiently, thus leading to stable and strong signals. The proposed method presents good ECL response for the detection of CEA allowing a wide linear range from 0.01 pg/mL to 10 ng/mL and a limit of detection as low as 3 fg/mL. The immunosensor would be a promising tool in the early diagnosis of CEA due to its high sensitivity, simplicity and cost-effective.

No MeSH data available.


ECL intensity of immunosensor with different electrochemical techniques including cyclic voltammetry (A) and single-step cycle pulse (B).The effect of initial potential (C), pulse potential (D), pulse period (E) and pulse time (F). Error bar = SD (n = 3).
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f4: ECL intensity of immunosensor with different electrochemical techniques including cyclic voltammetry (A) and single-step cycle pulse (B).The effect of initial potential (C), pulse potential (D), pulse period (E) and pulse time (F). Error bar = SD (n = 3).

Mentions: As far as we know ECL reaction is initiated by an electrochemical reaction at the electrode surface, the electrochemical parameters play very important roles in the ECL response. In this work, two different electrochemical techniques including cyclic voltammetry (Fig. 4A) and single-step cycle pulse (Fig. 4B) were investigated. The results showed that the ECL signals for the single-step cycle pulse were more stable and much stronger than that for cyclic voltammetry. The reasons might as follows. In the pattern of single-step cycle pulse, the diffusion layer on the surface of electrodes could be restored timely resulting in reproducible signals. Simultaneously, the electrode reaction with single-step cycle pulse could produce more photons than that for cyclic voltammetry with the same time, which causing stronger ECL signal.


Single-step cycle pulse operation of the label-free electrochemiluminescence immunosensor based on branched polypyrrole for carcinoembryonic antigen detection.

Zhu W, Wang Q, Ma H, Lv X, Wu D, Sun X, Du B, Wei Q - Sci Rep (2016)

ECL intensity of immunosensor with different electrochemical techniques including cyclic voltammetry (A) and single-step cycle pulse (B).The effect of initial potential (C), pulse potential (D), pulse period (E) and pulse time (F). Error bar = SD (n = 3).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4835776&req=5

f4: ECL intensity of immunosensor with different electrochemical techniques including cyclic voltammetry (A) and single-step cycle pulse (B).The effect of initial potential (C), pulse potential (D), pulse period (E) and pulse time (F). Error bar = SD (n = 3).
Mentions: As far as we know ECL reaction is initiated by an electrochemical reaction at the electrode surface, the electrochemical parameters play very important roles in the ECL response. In this work, two different electrochemical techniques including cyclic voltammetry (Fig. 4A) and single-step cycle pulse (Fig. 4B) were investigated. The results showed that the ECL signals for the single-step cycle pulse were more stable and much stronger than that for cyclic voltammetry. The reasons might as follows. In the pattern of single-step cycle pulse, the diffusion layer on the surface of electrodes could be restored timely resulting in reproducible signals. Simultaneously, the electrode reaction with single-step cycle pulse could produce more photons than that for cyclic voltammetry with the same time, which causing stronger ECL signal.

Bottom Line: Moreover, 1-butylpyridinium tetrafluroborate ([BPy]BF4) were used to disperse luminol functional-Au NPs@polypyrrole nanocomposites, resulting in the film-formation of composites on the electrode, which could improve the stability of immunosensor.The proposed method presents good ECL response for the detection of CEA allowing a wide linear range from 0.01 pg/mL to 10 ng/mL and a limit of detection as low as 3 fg/mL.The immunosensor would be a promising tool in the early diagnosis of CEA due to its high sensitivity, simplicity and cost-effective.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Chemical Sensing &Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, P.R. China.

ABSTRACT
A novel label-free electrochemiluminescence (ECL) immunosensor based on luminol functional-Au NPs@polypyrrole has been developed for the detection of carcinoembryonic antigen (CEA). In this work, polypyrrole prepared by chemical polymerization provided a large surface area to load amounts of gold nanoparticles (Au NPs). Au NPs could not only attach abundant luminol for the enhancement of ECL signal, but also provide a friendly microenvironment for the immobilization of antibodies. Moreover, 1-butylpyridinium tetrafluroborate ([BPy]BF4) were used to disperse luminol functional-Au NPs@polypyrrole nanocomposites, resulting in the film-formation of composites on the electrode, which could improve the stability of immunosensor. In particular, employment of single-step cycle pulse could limit the consecutive reaction between luminol and H2O2 efficiently, thus leading to stable and strong signals. The proposed method presents good ECL response for the detection of CEA allowing a wide linear range from 0.01 pg/mL to 10 ng/mL and a limit of detection as low as 3 fg/mL. The immunosensor would be a promising tool in the early diagnosis of CEA due to its high sensitivity, simplicity and cost-effective.

No MeSH data available.