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Single-step cycle pulse operation of the label-free electrochemiluminescence immunosensor based on branched polypyrrole for carcinoembryonic antigen detection.

Zhu W, Wang Q, Ma H, Lv X, Wu D, Sun X, Du B, Wei Q - Sci Rep (2016)

Bottom Line: Moreover, 1-butylpyridinium tetrafluroborate ([BPy]BF4) were used to disperse luminol functional-Au NPs@polypyrrole nanocomposites, resulting in the film-formation of composites on the electrode, which could improve the stability of immunosensor.The proposed method presents good ECL response for the detection of CEA allowing a wide linear range from 0.01 pg/mL to 10 ng/mL and a limit of detection as low as 3 fg/mL.The immunosensor would be a promising tool in the early diagnosis of CEA due to its high sensitivity, simplicity and cost-effective.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Chemical Sensing &Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, P.R. China.

ABSTRACT
A novel label-free electrochemiluminescence (ECL) immunosensor based on luminol functional-Au NPs@polypyrrole has been developed for the detection of carcinoembryonic antigen (CEA). In this work, polypyrrole prepared by chemical polymerization provided a large surface area to load amounts of gold nanoparticles (Au NPs). Au NPs could not only attach abundant luminol for the enhancement of ECL signal, but also provide a friendly microenvironment for the immobilization of antibodies. Moreover, 1-butylpyridinium tetrafluroborate ([BPy]BF4) were used to disperse luminol functional-Au NPs@polypyrrole nanocomposites, resulting in the film-formation of composites on the electrode, which could improve the stability of immunosensor. In particular, employment of single-step cycle pulse could limit the consecutive reaction between luminol and H2O2 efficiently, thus leading to stable and strong signals. The proposed method presents good ECL response for the detection of CEA allowing a wide linear range from 0.01 pg/mL to 10 ng/mL and a limit of detection as low as 3 fg/mL. The immunosensor would be a promising tool in the early diagnosis of CEA due to its high sensitivity, simplicity and cost-effective.

No MeSH data available.


SEM images of PPy (A) and Au NPs@PPy (B), (C) UV-vis spectrums of PPy (a), Au NPs (b), luminol (c), anti-CEA (d), anti-CEA-luminol-Au NPs@PPy (e). (D) The FT-IR characterization of PPy.
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f2: SEM images of PPy (A) and Au NPs@PPy (B), (C) UV-vis spectrums of PPy (a), Au NPs (b), luminol (c), anti-CEA (d), anti-CEA-luminol-Au NPs@PPy (e). (D) The FT-IR characterization of PPy.

Mentions: The morphologies of PPy and Au NPs@PPy were characterized by SEM. As shown in Fig. 2A, it could be observed visually that PPy was branched structure, showing uniform shapes and smooth surface. In addition, it was clear in Fig. 2B that a large number of Au NPs have been modified successfully on the surface of PPy. To further prove the luminol, anti-CEA, Au NPs and PPy were incubated together, the correlative UV-vis spectrums (Fig. 2C) were investigated. The maximum absorption peak of the as-synthesized PPy (curve a) was showed at 296 nm. Obvious absorption peak of luminol (curve c) was observed at around 300 nm and 348 nm. The characteristic absorption peak Au NPs (curve b) and anti-CEA (curve d) was identified at around 519 nm and 285 nm respectively. Curve e was the absorption curve of anti-CEA-luminol-Au NPs@PPy. As Fig. 2C (curve e) showed, the characteristic absorption peak of the luminol was apparently broadened and the major absorption peak of Au NPs also appeared, which illustrated that PPy, luminol, anti-CEA and Au NPs were incubated well effectively.


Single-step cycle pulse operation of the label-free electrochemiluminescence immunosensor based on branched polypyrrole for carcinoembryonic antigen detection.

Zhu W, Wang Q, Ma H, Lv X, Wu D, Sun X, Du B, Wei Q - Sci Rep (2016)

SEM images of PPy (A) and Au NPs@PPy (B), (C) UV-vis spectrums of PPy (a), Au NPs (b), luminol (c), anti-CEA (d), anti-CEA-luminol-Au NPs@PPy (e). (D) The FT-IR characterization of PPy.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4835776&req=5

f2: SEM images of PPy (A) and Au NPs@PPy (B), (C) UV-vis spectrums of PPy (a), Au NPs (b), luminol (c), anti-CEA (d), anti-CEA-luminol-Au NPs@PPy (e). (D) The FT-IR characterization of PPy.
Mentions: The morphologies of PPy and Au NPs@PPy were characterized by SEM. As shown in Fig. 2A, it could be observed visually that PPy was branched structure, showing uniform shapes and smooth surface. In addition, it was clear in Fig. 2B that a large number of Au NPs have been modified successfully on the surface of PPy. To further prove the luminol, anti-CEA, Au NPs and PPy were incubated together, the correlative UV-vis spectrums (Fig. 2C) were investigated. The maximum absorption peak of the as-synthesized PPy (curve a) was showed at 296 nm. Obvious absorption peak of luminol (curve c) was observed at around 300 nm and 348 nm. The characteristic absorption peak Au NPs (curve b) and anti-CEA (curve d) was identified at around 519 nm and 285 nm respectively. Curve e was the absorption curve of anti-CEA-luminol-Au NPs@PPy. As Fig. 2C (curve e) showed, the characteristic absorption peak of the luminol was apparently broadened and the major absorption peak of Au NPs also appeared, which illustrated that PPy, luminol, anti-CEA and Au NPs were incubated well effectively.

Bottom Line: Moreover, 1-butylpyridinium tetrafluroborate ([BPy]BF4) were used to disperse luminol functional-Au NPs@polypyrrole nanocomposites, resulting in the film-formation of composites on the electrode, which could improve the stability of immunosensor.The proposed method presents good ECL response for the detection of CEA allowing a wide linear range from 0.01 pg/mL to 10 ng/mL and a limit of detection as low as 3 fg/mL.The immunosensor would be a promising tool in the early diagnosis of CEA due to its high sensitivity, simplicity and cost-effective.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Chemical Sensing &Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, P.R. China.

ABSTRACT
A novel label-free electrochemiluminescence (ECL) immunosensor based on luminol functional-Au NPs@polypyrrole has been developed for the detection of carcinoembryonic antigen (CEA). In this work, polypyrrole prepared by chemical polymerization provided a large surface area to load amounts of gold nanoparticles (Au NPs). Au NPs could not only attach abundant luminol for the enhancement of ECL signal, but also provide a friendly microenvironment for the immobilization of antibodies. Moreover, 1-butylpyridinium tetrafluroborate ([BPy]BF4) were used to disperse luminol functional-Au NPs@polypyrrole nanocomposites, resulting in the film-formation of composites on the electrode, which could improve the stability of immunosensor. In particular, employment of single-step cycle pulse could limit the consecutive reaction between luminol and H2O2 efficiently, thus leading to stable and strong signals. The proposed method presents good ECL response for the detection of CEA allowing a wide linear range from 0.01 pg/mL to 10 ng/mL and a limit of detection as low as 3 fg/mL. The immunosensor would be a promising tool in the early diagnosis of CEA due to its high sensitivity, simplicity and cost-effective.

No MeSH data available.