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Single-step cycle pulse operation of the label-free electrochemiluminescence immunosensor based on branched polypyrrole for carcinoembryonic antigen detection.

Zhu W, Wang Q, Ma H, Lv X, Wu D, Sun X, Du B, Wei Q - Sci Rep (2016)

Bottom Line: Moreover, 1-butylpyridinium tetrafluroborate ([BPy]BF4) were used to disperse luminol functional-Au NPs@polypyrrole nanocomposites, resulting in the film-formation of composites on the electrode, which could improve the stability of immunosensor.The proposed method presents good ECL response for the detection of CEA allowing a wide linear range from 0.01 pg/mL to 10 ng/mL and a limit of detection as low as 3 fg/mL.The immunosensor would be a promising tool in the early diagnosis of CEA due to its high sensitivity, simplicity and cost-effective.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Chemical Sensing &Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, P.R. China.

ABSTRACT
A novel label-free electrochemiluminescence (ECL) immunosensor based on luminol functional-Au NPs@polypyrrole has been developed for the detection of carcinoembryonic antigen (CEA). In this work, polypyrrole prepared by chemical polymerization provided a large surface area to load amounts of gold nanoparticles (Au NPs). Au NPs could not only attach abundant luminol for the enhancement of ECL signal, but also provide a friendly microenvironment for the immobilization of antibodies. Moreover, 1-butylpyridinium tetrafluroborate ([BPy]BF4) were used to disperse luminol functional-Au NPs@polypyrrole nanocomposites, resulting in the film-formation of composites on the electrode, which could improve the stability of immunosensor. In particular, employment of single-step cycle pulse could limit the consecutive reaction between luminol and H2O2 efficiently, thus leading to stable and strong signals. The proposed method presents good ECL response for the detection of CEA allowing a wide linear range from 0.01 pg/mL to 10 ng/mL and a limit of detection as low as 3 fg/mL. The immunosensor would be a promising tool in the early diagnosis of CEA due to its high sensitivity, simplicity and cost-effective.

No MeSH data available.


(A) The preparation procedure of the anti-CEA-luminol-Au NPs@PPy. (B) The schematic illustration of the label-free electrochemiluminescence immunosensor.
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f1: (A) The preparation procedure of the anti-CEA-luminol-Au NPs@PPy. (B) The schematic illustration of the label-free electrochemiluminescence immunosensor.

Mentions: Herein, a promising label-free ECL immunosensor was fabricated, which was prepared by luminol-Au NPs@PPy as antibody carriers and sensing platform for the detection of CEA. The fabrication procedure of the immunosensor was shown in Fig. 1. PPy synthesized through a convenient synthetic route of chemical polymerization25 was used to immobilize Au NPs. Au NPs were used to combine with luminol and anti-CEA, and further to enhance the sensitivity of immunosensor. Considered of the excellent properties of ionic liquid, such as low toxicity, enhanced ionic conductivity, good chemical stability26, 1-butylpyridinium tetrafluroborate ([BPy]BF4) was adopted to disperse anti-CEA-luminol-Au NPs@PPy composites, which could not only result in the film-formation of composites for the immobilization on the electrode, but also improve the stability of immunosensor. Additionally, single-step cycle pulse was enforced to electrodes, causing that the fabricated sensing platform performed stable and strong ECL signals. The proposed label-free ECL immunosensor exhibited high sensitivity for CEA with a linear range from 0.01 pg/mL to 10 ng/mL and a low detection limit of 3 fg/mL. It would be promising and effective method for the recognition of target CEA.


Single-step cycle pulse operation of the label-free electrochemiluminescence immunosensor based on branched polypyrrole for carcinoembryonic antigen detection.

Zhu W, Wang Q, Ma H, Lv X, Wu D, Sun X, Du B, Wei Q - Sci Rep (2016)

(A) The preparation procedure of the anti-CEA-luminol-Au NPs@PPy. (B) The schematic illustration of the label-free electrochemiluminescence immunosensor.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4835776&req=5

f1: (A) The preparation procedure of the anti-CEA-luminol-Au NPs@PPy. (B) The schematic illustration of the label-free electrochemiluminescence immunosensor.
Mentions: Herein, a promising label-free ECL immunosensor was fabricated, which was prepared by luminol-Au NPs@PPy as antibody carriers and sensing platform for the detection of CEA. The fabrication procedure of the immunosensor was shown in Fig. 1. PPy synthesized through a convenient synthetic route of chemical polymerization25 was used to immobilize Au NPs. Au NPs were used to combine with luminol and anti-CEA, and further to enhance the sensitivity of immunosensor. Considered of the excellent properties of ionic liquid, such as low toxicity, enhanced ionic conductivity, good chemical stability26, 1-butylpyridinium tetrafluroborate ([BPy]BF4) was adopted to disperse anti-CEA-luminol-Au NPs@PPy composites, which could not only result in the film-formation of composites for the immobilization on the electrode, but also improve the stability of immunosensor. Additionally, single-step cycle pulse was enforced to electrodes, causing that the fabricated sensing platform performed stable and strong ECL signals. The proposed label-free ECL immunosensor exhibited high sensitivity for CEA with a linear range from 0.01 pg/mL to 10 ng/mL and a low detection limit of 3 fg/mL. It would be promising and effective method for the recognition of target CEA.

Bottom Line: Moreover, 1-butylpyridinium tetrafluroborate ([BPy]BF4) were used to disperse luminol functional-Au NPs@polypyrrole nanocomposites, resulting in the film-formation of composites on the electrode, which could improve the stability of immunosensor.The proposed method presents good ECL response for the detection of CEA allowing a wide linear range from 0.01 pg/mL to 10 ng/mL and a limit of detection as low as 3 fg/mL.The immunosensor would be a promising tool in the early diagnosis of CEA due to its high sensitivity, simplicity and cost-effective.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Chemical Sensing &Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, P.R. China.

ABSTRACT
A novel label-free electrochemiluminescence (ECL) immunosensor based on luminol functional-Au NPs@polypyrrole has been developed for the detection of carcinoembryonic antigen (CEA). In this work, polypyrrole prepared by chemical polymerization provided a large surface area to load amounts of gold nanoparticles (Au NPs). Au NPs could not only attach abundant luminol for the enhancement of ECL signal, but also provide a friendly microenvironment for the immobilization of antibodies. Moreover, 1-butylpyridinium tetrafluroborate ([BPy]BF4) were used to disperse luminol functional-Au NPs@polypyrrole nanocomposites, resulting in the film-formation of composites on the electrode, which could improve the stability of immunosensor. In particular, employment of single-step cycle pulse could limit the consecutive reaction between luminol and H2O2 efficiently, thus leading to stable and strong signals. The proposed method presents good ECL response for the detection of CEA allowing a wide linear range from 0.01 pg/mL to 10 ng/mL and a limit of detection as low as 3 fg/mL. The immunosensor would be a promising tool in the early diagnosis of CEA due to its high sensitivity, simplicity and cost-effective.

No MeSH data available.