Limits...
Clinicopathological Analysis and Multipronged Quantitative Proteomics Reveal Oxidative Stress and Cytoskeletal Proteins as Possible Markers for Severe Vivax Malaria.

Ray S, Patel SK, Venkatesh A, Bhave A, Kumar V, Singh V, Chatterjee G, Shah VG, Sharma S, Renu D, Nafis N, Gandhe P, Gogtay N, Thatte U, Sehgal K, Verma S, Karak A, Khanra D, Talukdar A, Kochar SK, S B V, Kochar DK, Rojh D, Varma SG, Gandhi MN, Srikanth R, Patankar S, Srivastava S - Sci Rep (2016)

Bottom Line: In Plasmodium vivax malaria, mechanisms that trigger transition from uncomplicated to fatal severe infections are obscure.Strikingly, unlike severe falciparum malaria the blood coagulation cascade was not found to be affected adversely in acute P. vivax infection.Our results suggest that Superoxide dismutase, Vitronectin, Titin, Apolipoprotein E, Serum amyloid A, and Haptoglobin are potential predictive markers for malaria severity.

View Article: PubMed Central - PubMed

Affiliation: Department of Biosciences and Bioengineering, Indian Institute of Technology Bombay, Powai, Mumbai 400076, India.

ABSTRACT
In Plasmodium vivax malaria, mechanisms that trigger transition from uncomplicated to fatal severe infections are obscure. In this multi-disciplinary study we have performed a comprehensive analysis of clinicopathological parameters and serum proteome profiles of vivax malaria patients with different severity levels of infection to investigate pathogenesis of severe malaria and identify surrogate markers of severity. Clinicopathological analysis and proteomics profiling has provided evidences for the modulation of diverse physiological pathways including oxidative stress, cytoskeletal regulation, lipid metabolism and complement cascades in severe malaria. Strikingly, unlike severe falciparum malaria the blood coagulation cascade was not found to be affected adversely in acute P. vivax infection. To the best of our knowledge, this is the first comprehensive proteomics study, which identified some possible cues for severe P. vivax infection. Our results suggest that Superoxide dismutase, Vitronectin, Titin, Apolipoprotein E, Serum amyloid A, and Haptoglobin are potential predictive markers for malaria severity.

No MeSH data available.


Related in: MedlinePlus

(A) Calibration–free concentration analysis of SAA in different study cohorts by using SPR. Measurement of SOD activity (B), and serum levels of thiobarbituric acid reactive substances (C) in NSVM and SVM patients. ** indicates p < 0.001, * indicates 0.001 < p < 0.05 and NS indicates p > 0.05 based on a Mann–Whitney U test.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4835765&req=5

f5: (A) Calibration–free concentration analysis of SAA in different study cohorts by using SPR. Measurement of SOD activity (B), and serum levels of thiobarbituric acid reactive substances (C) in NSVM and SVM patients. ** indicates p < 0.001, * indicates 0.001 < p < 0.05 and NS indicates p > 0.05 based on a Mann–Whitney U test.

Mentions: Calibration-free concentration analysis (CFCA) using SPR, which allows the measurement of functionally active target proteins, also indicated a progressive increase in serum abundance of SAA with increasing disease severity (Fig. 5A). SAA concentration measured in pooled serum of HC and DF patients was observed to be in a very comparable range (Table S9A). Interestingly, the measured concentrations of SAA in the pooled serum of both the NSVM and SVM patients were found to be significantly higher compared to HC (p < 0.005). Analysis of individual samples also presented similar results as obtained with the pooled samples (Table S9B). Nearly similar serum level of SAA was observed in the individual samples of HC and DF. In contrast, a significant increase in SAA level was observed in NSVM and SVM (p < 0.005) (Table S9B).


Clinicopathological Analysis and Multipronged Quantitative Proteomics Reveal Oxidative Stress and Cytoskeletal Proteins as Possible Markers for Severe Vivax Malaria.

Ray S, Patel SK, Venkatesh A, Bhave A, Kumar V, Singh V, Chatterjee G, Shah VG, Sharma S, Renu D, Nafis N, Gandhe P, Gogtay N, Thatte U, Sehgal K, Verma S, Karak A, Khanra D, Talukdar A, Kochar SK, S B V, Kochar DK, Rojh D, Varma SG, Gandhi MN, Srikanth R, Patankar S, Srivastava S - Sci Rep (2016)

(A) Calibration–free concentration analysis of SAA in different study cohorts by using SPR. Measurement of SOD activity (B), and serum levels of thiobarbituric acid reactive substances (C) in NSVM and SVM patients. ** indicates p < 0.001, * indicates 0.001 < p < 0.05 and NS indicates p > 0.05 based on a Mann–Whitney U test.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4835765&req=5

f5: (A) Calibration–free concentration analysis of SAA in different study cohorts by using SPR. Measurement of SOD activity (B), and serum levels of thiobarbituric acid reactive substances (C) in NSVM and SVM patients. ** indicates p < 0.001, * indicates 0.001 < p < 0.05 and NS indicates p > 0.05 based on a Mann–Whitney U test.
Mentions: Calibration-free concentration analysis (CFCA) using SPR, which allows the measurement of functionally active target proteins, also indicated a progressive increase in serum abundance of SAA with increasing disease severity (Fig. 5A). SAA concentration measured in pooled serum of HC and DF patients was observed to be in a very comparable range (Table S9A). Interestingly, the measured concentrations of SAA in the pooled serum of both the NSVM and SVM patients were found to be significantly higher compared to HC (p < 0.005). Analysis of individual samples also presented similar results as obtained with the pooled samples (Table S9B). Nearly similar serum level of SAA was observed in the individual samples of HC and DF. In contrast, a significant increase in SAA level was observed in NSVM and SVM (p < 0.005) (Table S9B).

Bottom Line: In Plasmodium vivax malaria, mechanisms that trigger transition from uncomplicated to fatal severe infections are obscure.Strikingly, unlike severe falciparum malaria the blood coagulation cascade was not found to be affected adversely in acute P. vivax infection.Our results suggest that Superoxide dismutase, Vitronectin, Titin, Apolipoprotein E, Serum amyloid A, and Haptoglobin are potential predictive markers for malaria severity.

View Article: PubMed Central - PubMed

Affiliation: Department of Biosciences and Bioengineering, Indian Institute of Technology Bombay, Powai, Mumbai 400076, India.

ABSTRACT
In Plasmodium vivax malaria, mechanisms that trigger transition from uncomplicated to fatal severe infections are obscure. In this multi-disciplinary study we have performed a comprehensive analysis of clinicopathological parameters and serum proteome profiles of vivax malaria patients with different severity levels of infection to investigate pathogenesis of severe malaria and identify surrogate markers of severity. Clinicopathological analysis and proteomics profiling has provided evidences for the modulation of diverse physiological pathways including oxidative stress, cytoskeletal regulation, lipid metabolism and complement cascades in severe malaria. Strikingly, unlike severe falciparum malaria the blood coagulation cascade was not found to be affected adversely in acute P. vivax infection. To the best of our knowledge, this is the first comprehensive proteomics study, which identified some possible cues for severe P. vivax infection. Our results suggest that Superoxide dismutase, Vitronectin, Titin, Apolipoprotein E, Serum amyloid A, and Haptoglobin are potential predictive markers for malaria severity.

No MeSH data available.


Related in: MedlinePlus