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Clinicopathological Analysis and Multipronged Quantitative Proteomics Reveal Oxidative Stress and Cytoskeletal Proteins as Possible Markers for Severe Vivax Malaria.

Ray S, Patel SK, Venkatesh A, Bhave A, Kumar V, Singh V, Chatterjee G, Shah VG, Sharma S, Renu D, Nafis N, Gandhe P, Gogtay N, Thatte U, Sehgal K, Verma S, Karak A, Khanra D, Talukdar A, Kochar SK, S B V, Kochar DK, Rojh D, Varma SG, Gandhi MN, Srikanth R, Patankar S, Srivastava S - Sci Rep (2016)

Bottom Line: In Plasmodium vivax malaria, mechanisms that trigger transition from uncomplicated to fatal severe infections are obscure.Strikingly, unlike severe falciparum malaria the blood coagulation cascade was not found to be affected adversely in acute P. vivax infection.Our results suggest that Superoxide dismutase, Vitronectin, Titin, Apolipoprotein E, Serum amyloid A, and Haptoglobin are potential predictive markers for malaria severity.

View Article: PubMed Central - PubMed

Affiliation: Department of Biosciences and Bioengineering, Indian Institute of Technology Bombay, Powai, Mumbai 400076, India.

ABSTRACT
In Plasmodium vivax malaria, mechanisms that trigger transition from uncomplicated to fatal severe infections are obscure. In this multi-disciplinary study we have performed a comprehensive analysis of clinicopathological parameters and serum proteome profiles of vivax malaria patients with different severity levels of infection to investigate pathogenesis of severe malaria and identify surrogate markers of severity. Clinicopathological analysis and proteomics profiling has provided evidences for the modulation of diverse physiological pathways including oxidative stress, cytoskeletal regulation, lipid metabolism and complement cascades in severe malaria. Strikingly, unlike severe falciparum malaria the blood coagulation cascade was not found to be affected adversely in acute P. vivax infection. To the best of our knowledge, this is the first comprehensive proteomics study, which identified some possible cues for severe P. vivax infection. Our results suggest that Superoxide dismutase, Vitronectin, Titin, Apolipoprotein E, Serum amyloid A, and Haptoglobin are potential predictive markers for malaria severity.

No MeSH data available.


Related in: MedlinePlus

Overview of the modulated physiological pathways and panels of differentially abundant proteins in NSVM and SVM.Red, up-regulated; Green, down-regulated proteins in vivax malaria.
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f3: Overview of the modulated physiological pathways and panels of differentially abundant proteins in NSVM and SVM.Red, up-regulated; Green, down-regulated proteins in vivax malaria.

Mentions: PANTHER (Protein ANalysis THrough Evolutionary Relationships) analysis also indicated involvement of the differentially abundant proteins in blood coagulation system. In addition, plasminogen activating cascade, apoptosis signaling, inflammation mediated by chemokine and cytokine signaling pathways were identified as the related physiological pathways with statistical significance (pā€‰<ā€‰0.05) (Table S6). In DAVID (Database for Annotation, Visualization and Integrated Discovery) analysis complement and coagulation cascades, hemostasis, plasminogen activating cascade, metabolism of lipids and lipoproteins were identified as the significant pathways (Table S6). Taken together, bioinformatics analysis indicated that both severe and non-severe P. vivax infections lead to alteration of serum levels several proteins involved in diverse essential physiological pathways including acute phase response signaling, complement cascades, lipid transport and metabolism and blood coagulation (Table 3; Fig. 3).


Clinicopathological Analysis and Multipronged Quantitative Proteomics Reveal Oxidative Stress and Cytoskeletal Proteins as Possible Markers for Severe Vivax Malaria.

Ray S, Patel SK, Venkatesh A, Bhave A, Kumar V, Singh V, Chatterjee G, Shah VG, Sharma S, Renu D, Nafis N, Gandhe P, Gogtay N, Thatte U, Sehgal K, Verma S, Karak A, Khanra D, Talukdar A, Kochar SK, S B V, Kochar DK, Rojh D, Varma SG, Gandhi MN, Srikanth R, Patankar S, Srivastava S - Sci Rep (2016)

Overview of the modulated physiological pathways and panels of differentially abundant proteins in NSVM and SVM.Red, up-regulated; Green, down-regulated proteins in vivax malaria.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4835765&req=5

f3: Overview of the modulated physiological pathways and panels of differentially abundant proteins in NSVM and SVM.Red, up-regulated; Green, down-regulated proteins in vivax malaria.
Mentions: PANTHER (Protein ANalysis THrough Evolutionary Relationships) analysis also indicated involvement of the differentially abundant proteins in blood coagulation system. In addition, plasminogen activating cascade, apoptosis signaling, inflammation mediated by chemokine and cytokine signaling pathways were identified as the related physiological pathways with statistical significance (pā€‰<ā€‰0.05) (Table S6). In DAVID (Database for Annotation, Visualization and Integrated Discovery) analysis complement and coagulation cascades, hemostasis, plasminogen activating cascade, metabolism of lipids and lipoproteins were identified as the significant pathways (Table S6). Taken together, bioinformatics analysis indicated that both severe and non-severe P. vivax infections lead to alteration of serum levels several proteins involved in diverse essential physiological pathways including acute phase response signaling, complement cascades, lipid transport and metabolism and blood coagulation (Table 3; Fig. 3).

Bottom Line: In Plasmodium vivax malaria, mechanisms that trigger transition from uncomplicated to fatal severe infections are obscure.Strikingly, unlike severe falciparum malaria the blood coagulation cascade was not found to be affected adversely in acute P. vivax infection.Our results suggest that Superoxide dismutase, Vitronectin, Titin, Apolipoprotein E, Serum amyloid A, and Haptoglobin are potential predictive markers for malaria severity.

View Article: PubMed Central - PubMed

Affiliation: Department of Biosciences and Bioengineering, Indian Institute of Technology Bombay, Powai, Mumbai 400076, India.

ABSTRACT
In Plasmodium vivax malaria, mechanisms that trigger transition from uncomplicated to fatal severe infections are obscure. In this multi-disciplinary study we have performed a comprehensive analysis of clinicopathological parameters and serum proteome profiles of vivax malaria patients with different severity levels of infection to investigate pathogenesis of severe malaria and identify surrogate markers of severity. Clinicopathological analysis and proteomics profiling has provided evidences for the modulation of diverse physiological pathways including oxidative stress, cytoskeletal regulation, lipid metabolism and complement cascades in severe malaria. Strikingly, unlike severe falciparum malaria the blood coagulation cascade was not found to be affected adversely in acute P. vivax infection. To the best of our knowledge, this is the first comprehensive proteomics study, which identified some possible cues for severe P. vivax infection. Our results suggest that Superoxide dismutase, Vitronectin, Titin, Apolipoprotein E, Serum amyloid A, and Haptoglobin are potential predictive markers for malaria severity.

No MeSH data available.


Related in: MedlinePlus