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The role of silicon in enhancing resistance to bacterial blight of hydroponic- and soil-cultured rice.

Song A, Xue G, Cui P, Fan F, Liu H, Yin C, Sun W, Liang Y - Sci Rep (2016)

Bottom Line: In both inoculated and uninoculated plants, shoot dry weight was significantly higher in the +Si plants than in the -Si plants.Moreover, total concentrations of soluble phenolics and lignin in rice leaves were significantly higher in the +Si plants than in the -Si plants.Addition of Si resulted in increased Pal transcription, and inhibited CatA and Os03g0126000 expression in the earlier and later stages of bacterial inoculation, respectively.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Plant Nutrition and Fertilizer, Ministry of Agriculture, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R. China.

ABSTRACT
Here we report for the first time that bacterial blight of rice can be alleviated by silicon (Si) added. In both inoculated and uninoculated plants, shoot dry weight was significantly higher in the +Si plants than in the -Si plants. A soil-cultured trial showed that disease severity was 24.3% lower in the Si-amended plants than in the non-Si-amended plants. Plants that were switched from -Si to +Si nutrient solution and simultaneously inoculated with Xoo also exhibited the same high resistance to bacterial blight as the plants that were treated continuously with Si, with control efficiencies of 52.8 and 62.9%, respectively. Moreover, total concentrations of soluble phenolics and lignin in rice leaves were significantly higher in the +Si plants than in the -Si plants. Polyphenoloxidase (PPO) and phenylalanine ammonia-lyase (PAL) activities in rice leaves were observed to be higher in the +Si plants than in the -Si plants. The expression levels of Os03g0109600, Prla, Rcht2 and Lox2osPil, were also higher in +Si plants than in -Si plants post-inoculation during the experimental time. Addition of Si resulted in increased Pal transcription, and inhibited CatA and Os03g0126000 expression in the earlier and later stages of bacterial inoculation, respectively.

No MeSH data available.


Related in: MedlinePlus

Relative expression levels of (A) Os03g0109600, (B) Os03g0126000, (C) Pal, (D) Prla, (E) Rcht2, (F) Lox2osPil, and (G) CatA genes in leaves of rice seedlings amended with silicon (+Si) or not (−Si) post-inoculation with Xoo. The values are means of three replications. Vertical lines represent standard deviations. Asterisks denote significant difference at P < 0.05 between +Si and −Si treatments at a same time-point according to Student’s t-test.
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f5: Relative expression levels of (A) Os03g0109600, (B) Os03g0126000, (C) Pal, (D) Prla, (E) Rcht2, (F) Lox2osPil, and (G) CatA genes in leaves of rice seedlings amended with silicon (+Si) or not (−Si) post-inoculation with Xoo. The values are means of three replications. Vertical lines represent standard deviations. Asterisks denote significant difference at P < 0.05 between +Si and −Si treatments at a same time-point according to Student’s t-test.

Mentions: Os03g0109600 gene encodes a protein which consists of 175 amino acids and contains nuclear localization signal and NAC conserved domain database. In the hydroponic experiment, there was a difference in Os03g0109600 expression levels between the +Si plants and the −Si plants post inoculation. The expression levels at 6 and 24 hpi were 4.6 and 22.3 times higher, respectively, in the presence of Si than in the absence of Si (Fig. 5A). The Os03g0109600 gene might play an important role in resistance to the bacterial blight of rice. The addition of Si could activate Os03g0109600 gene expression and enhance the activity of transcription factors, consequently increasing disease resistance.


The role of silicon in enhancing resistance to bacterial blight of hydroponic- and soil-cultured rice.

Song A, Xue G, Cui P, Fan F, Liu H, Yin C, Sun W, Liang Y - Sci Rep (2016)

Relative expression levels of (A) Os03g0109600, (B) Os03g0126000, (C) Pal, (D) Prla, (E) Rcht2, (F) Lox2osPil, and (G) CatA genes in leaves of rice seedlings amended with silicon (+Si) or not (−Si) post-inoculation with Xoo. The values are means of three replications. Vertical lines represent standard deviations. Asterisks denote significant difference at P < 0.05 between +Si and −Si treatments at a same time-point according to Student’s t-test.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4835757&req=5

f5: Relative expression levels of (A) Os03g0109600, (B) Os03g0126000, (C) Pal, (D) Prla, (E) Rcht2, (F) Lox2osPil, and (G) CatA genes in leaves of rice seedlings amended with silicon (+Si) or not (−Si) post-inoculation with Xoo. The values are means of three replications. Vertical lines represent standard deviations. Asterisks denote significant difference at P < 0.05 between +Si and −Si treatments at a same time-point according to Student’s t-test.
Mentions: Os03g0109600 gene encodes a protein which consists of 175 amino acids and contains nuclear localization signal and NAC conserved domain database. In the hydroponic experiment, there was a difference in Os03g0109600 expression levels between the +Si plants and the −Si plants post inoculation. The expression levels at 6 and 24 hpi were 4.6 and 22.3 times higher, respectively, in the presence of Si than in the absence of Si (Fig. 5A). The Os03g0109600 gene might play an important role in resistance to the bacterial blight of rice. The addition of Si could activate Os03g0109600 gene expression and enhance the activity of transcription factors, consequently increasing disease resistance.

Bottom Line: In both inoculated and uninoculated plants, shoot dry weight was significantly higher in the +Si plants than in the -Si plants.Moreover, total concentrations of soluble phenolics and lignin in rice leaves were significantly higher in the +Si plants than in the -Si plants.Addition of Si resulted in increased Pal transcription, and inhibited CatA and Os03g0126000 expression in the earlier and later stages of bacterial inoculation, respectively.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Plant Nutrition and Fertilizer, Ministry of Agriculture, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R. China.

ABSTRACT
Here we report for the first time that bacterial blight of rice can be alleviated by silicon (Si) added. In both inoculated and uninoculated plants, shoot dry weight was significantly higher in the +Si plants than in the -Si plants. A soil-cultured trial showed that disease severity was 24.3% lower in the Si-amended plants than in the non-Si-amended plants. Plants that were switched from -Si to +Si nutrient solution and simultaneously inoculated with Xoo also exhibited the same high resistance to bacterial blight as the plants that were treated continuously with Si, with control efficiencies of 52.8 and 62.9%, respectively. Moreover, total concentrations of soluble phenolics and lignin in rice leaves were significantly higher in the +Si plants than in the -Si plants. Polyphenoloxidase (PPO) and phenylalanine ammonia-lyase (PAL) activities in rice leaves were observed to be higher in the +Si plants than in the -Si plants. The expression levels of Os03g0109600, Prla, Rcht2 and Lox2osPil, were also higher in +Si plants than in -Si plants post-inoculation during the experimental time. Addition of Si resulted in increased Pal transcription, and inhibited CatA and Os03g0126000 expression in the earlier and later stages of bacterial inoculation, respectively.

No MeSH data available.


Related in: MedlinePlus