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The role of silicon in enhancing resistance to bacterial blight of hydroponic- and soil-cultured rice.

Song A, Xue G, Cui P, Fan F, Liu H, Yin C, Sun W, Liang Y - Sci Rep (2016)

Bottom Line: In both inoculated and uninoculated plants, shoot dry weight was significantly higher in the +Si plants than in the -Si plants.Moreover, total concentrations of soluble phenolics and lignin in rice leaves were significantly higher in the +Si plants than in the -Si plants.Addition of Si resulted in increased Pal transcription, and inhibited CatA and Os03g0126000 expression in the earlier and later stages of bacterial inoculation, respectively.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Plant Nutrition and Fertilizer, Ministry of Agriculture, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R. China.

ABSTRACT
Here we report for the first time that bacterial blight of rice can be alleviated by silicon (Si) added. In both inoculated and uninoculated plants, shoot dry weight was significantly higher in the +Si plants than in the -Si plants. A soil-cultured trial showed that disease severity was 24.3% lower in the Si-amended plants than in the non-Si-amended plants. Plants that were switched from -Si to +Si nutrient solution and simultaneously inoculated with Xoo also exhibited the same high resistance to bacterial blight as the plants that were treated continuously with Si, with control efficiencies of 52.8 and 62.9%, respectively. Moreover, total concentrations of soluble phenolics and lignin in rice leaves were significantly higher in the +Si plants than in the -Si plants. Polyphenoloxidase (PPO) and phenylalanine ammonia-lyase (PAL) activities in rice leaves were observed to be higher in the +Si plants than in the -Si plants. The expression levels of Os03g0109600, Prla, Rcht2 and Lox2osPil, were also higher in +Si plants than in -Si plants post-inoculation during the experimental time. Addition of Si resulted in increased Pal transcription, and inhibited CatA and Os03g0126000 expression in the earlier and later stages of bacterial inoculation, respectively.

No MeSH data available.


Related in: MedlinePlus

Changes in concentrations of total soluble phenolics (A) and lignin (B) in leaves of rice seedlings amended with silicon (+Si) or not (−Si) post-inoculation with Xoo. The values are means of three replications. Vertical lines represent standard deviations. Asterisks denote significant difference at P < 0.05 between +Si and −Si treatments at a same time-point according to Student’s t-test.
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f4: Changes in concentrations of total soluble phenolics (A) and lignin (B) in leaves of rice seedlings amended with silicon (+Si) or not (−Si) post-inoculation with Xoo. The values are means of three replications. Vertical lines represent standard deviations. Asterisks denote significant difference at P < 0.05 between +Si and −Si treatments at a same time-point according to Student’s t-test.

Mentions: In the hydroponic experiment, PAL activity in leaves was significantly higher in the +Si plants than in the −Si plants throughout the experimental period, except at 72 h and 120 h post-inoculation (hpi). The PAL activity in both the +Si plants and the −Si plants reached a peak at 24 hpi (Fig. 3A). The enzyme activity then began to decline in both the +Si plants and the −Si plants. The PPO activity in rice leaves was higher in the +Si plants than in the −Si plants throughout the hydroponic experimental period, although this was significant only at 72 and 120 hpi (Fig. 3B). The PPO activity in both the +Si plants and the −Si plants reached a peak at 48 hpi (Fig. 3B). The level of total soluble phenolics in rice leaves was significantly higher in the +Si plants than in the −Si plants at 1–6 d post-inoculation (dpi) (Fig. 4A). The concentration of total soluble phenolics in both +Si and −Si plants reached a maximum at 3 dpi (Fig. 4A). The concentration of lignin in rice leaves was significantly higher in the +Si plants than in the −Si plants at 2, 3, 4 and 5 dpi (Fig. 4B). However, there was no significant difference between the +Si plants and the −Si plants at 1 dpi (Fig. 4B). The concentration of lignin in both the +Si plants and the −Si plants reached their maximum at 4 dpi (Fig. 4B).


The role of silicon in enhancing resistance to bacterial blight of hydroponic- and soil-cultured rice.

Song A, Xue G, Cui P, Fan F, Liu H, Yin C, Sun W, Liang Y - Sci Rep (2016)

Changes in concentrations of total soluble phenolics (A) and lignin (B) in leaves of rice seedlings amended with silicon (+Si) or not (−Si) post-inoculation with Xoo. The values are means of three replications. Vertical lines represent standard deviations. Asterisks denote significant difference at P < 0.05 between +Si and −Si treatments at a same time-point according to Student’s t-test.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4835757&req=5

f4: Changes in concentrations of total soluble phenolics (A) and lignin (B) in leaves of rice seedlings amended with silicon (+Si) or not (−Si) post-inoculation with Xoo. The values are means of three replications. Vertical lines represent standard deviations. Asterisks denote significant difference at P < 0.05 between +Si and −Si treatments at a same time-point according to Student’s t-test.
Mentions: In the hydroponic experiment, PAL activity in leaves was significantly higher in the +Si plants than in the −Si plants throughout the experimental period, except at 72 h and 120 h post-inoculation (hpi). The PAL activity in both the +Si plants and the −Si plants reached a peak at 24 hpi (Fig. 3A). The enzyme activity then began to decline in both the +Si plants and the −Si plants. The PPO activity in rice leaves was higher in the +Si plants than in the −Si plants throughout the hydroponic experimental period, although this was significant only at 72 and 120 hpi (Fig. 3B). The PPO activity in both the +Si plants and the −Si plants reached a peak at 48 hpi (Fig. 3B). The level of total soluble phenolics in rice leaves was significantly higher in the +Si plants than in the −Si plants at 1–6 d post-inoculation (dpi) (Fig. 4A). The concentration of total soluble phenolics in both +Si and −Si plants reached a maximum at 3 dpi (Fig. 4A). The concentration of lignin in rice leaves was significantly higher in the +Si plants than in the −Si plants at 2, 3, 4 and 5 dpi (Fig. 4B). However, there was no significant difference between the +Si plants and the −Si plants at 1 dpi (Fig. 4B). The concentration of lignin in both the +Si plants and the −Si plants reached their maximum at 4 dpi (Fig. 4B).

Bottom Line: In both inoculated and uninoculated plants, shoot dry weight was significantly higher in the +Si plants than in the -Si plants.Moreover, total concentrations of soluble phenolics and lignin in rice leaves were significantly higher in the +Si plants than in the -Si plants.Addition of Si resulted in increased Pal transcription, and inhibited CatA and Os03g0126000 expression in the earlier and later stages of bacterial inoculation, respectively.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Plant Nutrition and Fertilizer, Ministry of Agriculture, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R. China.

ABSTRACT
Here we report for the first time that bacterial blight of rice can be alleviated by silicon (Si) added. In both inoculated and uninoculated plants, shoot dry weight was significantly higher in the +Si plants than in the -Si plants. A soil-cultured trial showed that disease severity was 24.3% lower in the Si-amended plants than in the non-Si-amended plants. Plants that were switched from -Si to +Si nutrient solution and simultaneously inoculated with Xoo also exhibited the same high resistance to bacterial blight as the plants that were treated continuously with Si, with control efficiencies of 52.8 and 62.9%, respectively. Moreover, total concentrations of soluble phenolics and lignin in rice leaves were significantly higher in the +Si plants than in the -Si plants. Polyphenoloxidase (PPO) and phenylalanine ammonia-lyase (PAL) activities in rice leaves were observed to be higher in the +Si plants than in the -Si plants. The expression levels of Os03g0109600, Prla, Rcht2 and Lox2osPil, were also higher in +Si plants than in -Si plants post-inoculation during the experimental time. Addition of Si resulted in increased Pal transcription, and inhibited CatA and Os03g0126000 expression in the earlier and later stages of bacterial inoculation, respectively.

No MeSH data available.


Related in: MedlinePlus