Limits...
Correction to Letter: Fluorinated Aromatic Amino Acids Are Sensitive (19)F NMR Probes for Bromodomain-Ligand Interactions.

Mishra NK, Urick AK, Ember SW, Schönbrunn E, Pomerantz WC - ACS Chem. Biol. (2016)

View Article: PubMed Central - PubMed

AUTOMATICALLY GENERATED EXCERPT
Please rate it.

This correction regards a typographical error found in Figure 3B of the recent letter in the December issue of ACS Chemical resonances from aromatic amino acids located on the bromodomain to A typographical error has been found in Figure 3B which depicts a F NMR spectrum of a 3-fluorotyrosine-labeled Brd4 bromodomain (3FY-Brd4)... for the most downfield resonance at −128 ppm labeled Y118 and the resonance at −138 ppm labeled as Y119 have been interchanged... assignments and analyses in the Supporting Information remain correct in addition to analyses in the main text... The typographical error does not change any conclusions made in the manuscript.

No MeSH data available.


PrOF NMR titrationof (+)-JQ1 with Brd4(1). (A) Bottom to top:5FW-Brd4(1) (25 μM), with 1 equiv (+)-JQ1, with 5FW-BPTF (25μM), with 5FW-BPTF (25 μM) and 1 equiv (+)-JQ1, with unlabeledBrdT(1) (50 μM) and 1 equiv (+)-JQ1. (B) Bottom to Top: 3FY-Brd4(1)(47 μM) titrated with 2 equiv (+)-JQ1.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4835746&req=5

fig3: PrOF NMR titrationof (+)-JQ1 with Brd4(1). (A) Bottom to top:5FW-Brd4(1) (25 μM), with 1 equiv (+)-JQ1, with 5FW-BPTF (25μM), with 5FW-BPTF (25 μM) and 1 equiv (+)-JQ1, with unlabeledBrdT(1) (50 μM) and 1 equiv (+)-JQ1. (B) Bottom to Top: 3FY-Brd4(1)(47 μM) titrated with 2 equiv (+)-JQ1.

Mentions: This correction regards a typographicalerror found in Figure 3B of the recent letter in the December issue of ACS ChemicalBiology (ACS Chem. Biol., 2014, 9, 2755–2760) by Pomerantz and co-workers. In this report,the authors use chemical shift perturbations of assigned fluorineresonances from aromatic amino acids located on the bromodomain toquantify binding affinity and characterize a binding surface. A typographicalerror has been found in Figure 3B which depicts a 19F NMR spectrum of a 3-fluorotyrosine-labeledBrd4 bromodomain (3FY-Brd4). The assignments in the original versionfor the most downfield resonance at −128 ppm labeled Y118 andthe resonance at −138 ppm labeled as Y119 have been interchanged.The correct assignments are shown in the figure above. All of theassignments and analyses in the Supporting Information remain correctin addition to analyses in the main text. The typographical errordoes not change any conclusions made in the manuscript.


Correction to Letter: Fluorinated Aromatic Amino Acids Are Sensitive (19)F NMR Probes for Bromodomain-Ligand Interactions.

Mishra NK, Urick AK, Ember SW, Schönbrunn E, Pomerantz WC - ACS Chem. Biol. (2016)

PrOF NMR titrationof (+)-JQ1 with Brd4(1). (A) Bottom to top:5FW-Brd4(1) (25 μM), with 1 equiv (+)-JQ1, with 5FW-BPTF (25μM), with 5FW-BPTF (25 μM) and 1 equiv (+)-JQ1, with unlabeledBrdT(1) (50 μM) and 1 equiv (+)-JQ1. (B) Bottom to Top: 3FY-Brd4(1)(47 μM) titrated with 2 equiv (+)-JQ1.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4835746&req=5

fig3: PrOF NMR titrationof (+)-JQ1 with Brd4(1). (A) Bottom to top:5FW-Brd4(1) (25 μM), with 1 equiv (+)-JQ1, with 5FW-BPTF (25μM), with 5FW-BPTF (25 μM) and 1 equiv (+)-JQ1, with unlabeledBrdT(1) (50 μM) and 1 equiv (+)-JQ1. (B) Bottom to Top: 3FY-Brd4(1)(47 μM) titrated with 2 equiv (+)-JQ1.
Mentions: This correction regards a typographicalerror found in Figure 3B of the recent letter in the December issue of ACS ChemicalBiology (ACS Chem. Biol., 2014, 9, 2755–2760) by Pomerantz and co-workers. In this report,the authors use chemical shift perturbations of assigned fluorineresonances from aromatic amino acids located on the bromodomain toquantify binding affinity and characterize a binding surface. A typographicalerror has been found in Figure 3B which depicts a 19F NMR spectrum of a 3-fluorotyrosine-labeledBrd4 bromodomain (3FY-Brd4). The assignments in the original versionfor the most downfield resonance at −128 ppm labeled Y118 andthe resonance at −138 ppm labeled as Y119 have been interchanged.The correct assignments are shown in the figure above. All of theassignments and analyses in the Supporting Information remain correctin addition to analyses in the main text. The typographical errordoes not change any conclusions made in the manuscript.

View Article: PubMed Central - PubMed

AUTOMATICALLY GENERATED EXCERPT
Please rate it.

This correction regards a typographical error found in Figure 3B of the recent letter in the December issue of ACS Chemical resonances from aromatic amino acids located on the bromodomain to A typographical error has been found in Figure 3B which depicts a F NMR spectrum of a 3-fluorotyrosine-labeled Brd4 bromodomain (3FY-Brd4)... for the most downfield resonance at −128 ppm labeled Y118 and the resonance at −138 ppm labeled as Y119 have been interchanged... assignments and analyses in the Supporting Information remain correct in addition to analyses in the main text... The typographical error does not change any conclusions made in the manuscript.

No MeSH data available.