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The Asian arowana (Scleropages formosus) genome provides new insights into the evolution of an early lineage of teleosts.

Bian C, Hu Y, Ravi V, Kuznetsova IS, Shen X, Mu X, Sun Y, You X, Li J, Li X, Qiu Y, Tay BH, Thevasagayam NM, Komissarov AS, Trifonov V, Kabilov M, Tupikin A, Luo J, Liu Y, Song H, Liu C, Wang X, Gu D, Yang Y, Li W, Polgar G, Fan G, Zeng P, Zhang H, Xiong Z, Tang Z, Peng C, Ruan Z, Yu H, Chen J, Fan M, Huang Y, Wang M, Zhao X, Hu G, Yang H, Wang J, Wang J, Xu X, Song L, Xu G, Xu P, Xu J, O'Brien SJ, Orbán L, Venkatesh B, Shi Q - Sci Rep (2016)

Bottom Line: Differential gene expression among three varieties provides insights into the genetic basis of colour variation.A potential heterogametic sex chromosome is identified in the female arowana karyotype, suggesting that the sex is determined by a ZW/ZZ sex chromosomal system.The high-quality reference genome of the golden arowana and the draft assemblies of the red and green varieties are valuable resources for understanding the biology, adaptation and behaviour of Asian arowanas.

View Article: PubMed Central - PubMed

Affiliation: Shenzhen Key Lab of Marine Genomics, Guangdong Provincial Key Lab of Molecular Breeding in Marine Economic Animals, Shenzhen 518083, China.

ABSTRACT
The Asian arowana (Scleropages formosus), one of the world's most expensive cultivated ornamental fishes, is an endangered species. It represents an ancient lineage of teleosts: the Osteoglossomorpha. Here, we provide a high-quality chromosome-level reference genome of a female golden-variety arowana using a combination of deep shotgun sequencing and high-resolution linkage mapping. In addition, we have also generated two draft genome assemblies for the red and green varieties. Phylogenomic analysis supports a sister group relationship between Osteoglossomorpha (bonytongues) and Elopomorpha (eels and relatives), with the two clades together forming a sister group of Clupeocephala which includes all the remaining teleosts. The arowana genome retains the full complement of eight Hox clusters unlike the African butterfly fish (Pantodon buchholzi), another bonytongue fish, which possess only five Hox clusters. Differential gene expression among three varieties provides insights into the genetic basis of colour variation. A potential heterogametic sex chromosome is identified in the female arowana karyotype, suggesting that the sex is determined by a ZW/ZZ sex chromosomal system. The high-quality reference genome of the golden arowana and the draft assemblies of the red and green varieties are valuable resources for understanding the biology, adaptation and behaviour of Asian arowanas.

No MeSH data available.


Related in: MedlinePlus

The three colour varieties of Asian arowana sequenced in this study.(A) golden, (B) red and (C) green variety. These are among the most expensive ornamental fishes in the world (young adults for red arowanas cost from $1500 to $2000). The value of the fish depends on the colour with the red variety fetching the highest price.
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f1: The three colour varieties of Asian arowana sequenced in this study.(A) golden, (B) red and (C) green variety. These are among the most expensive ornamental fishes in the world (young adults for red arowanas cost from $1500 to $2000). The value of the fish depends on the colour with the red variety fetching the highest price.

Mentions: In this study, we have generated a high quality, chromosome-level reference genome of a golden female arowana (Fig. 1A), in addition to draft genome sequences of red and green arowana varieties (Fig. 1B,C). Based on a stringent, genome-scale set of one-to-one orthologues from various teleosts, we report a robust phylogenomic analysis which resolves the branching order of the three major lineages of teleosts. Our study also identifies differentially expressed genes potentially involved in the colour variations, and provides the first indication for the likely presence of a ZW/ZZ sex chromosomal system in the species.


The Asian arowana (Scleropages formosus) genome provides new insights into the evolution of an early lineage of teleosts.

Bian C, Hu Y, Ravi V, Kuznetsova IS, Shen X, Mu X, Sun Y, You X, Li J, Li X, Qiu Y, Tay BH, Thevasagayam NM, Komissarov AS, Trifonov V, Kabilov M, Tupikin A, Luo J, Liu Y, Song H, Liu C, Wang X, Gu D, Yang Y, Li W, Polgar G, Fan G, Zeng P, Zhang H, Xiong Z, Tang Z, Peng C, Ruan Z, Yu H, Chen J, Fan M, Huang Y, Wang M, Zhao X, Hu G, Yang H, Wang J, Wang J, Xu X, Song L, Xu G, Xu P, Xu J, O'Brien SJ, Orbán L, Venkatesh B, Shi Q - Sci Rep (2016)

The three colour varieties of Asian arowana sequenced in this study.(A) golden, (B) red and (C) green variety. These are among the most expensive ornamental fishes in the world (young adults for red arowanas cost from $1500 to $2000). The value of the fish depends on the colour with the red variety fetching the highest price.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4835728&req=5

f1: The three colour varieties of Asian arowana sequenced in this study.(A) golden, (B) red and (C) green variety. These are among the most expensive ornamental fishes in the world (young adults for red arowanas cost from $1500 to $2000). The value of the fish depends on the colour with the red variety fetching the highest price.
Mentions: In this study, we have generated a high quality, chromosome-level reference genome of a golden female arowana (Fig. 1A), in addition to draft genome sequences of red and green arowana varieties (Fig. 1B,C). Based on a stringent, genome-scale set of one-to-one orthologues from various teleosts, we report a robust phylogenomic analysis which resolves the branching order of the three major lineages of teleosts. Our study also identifies differentially expressed genes potentially involved in the colour variations, and provides the first indication for the likely presence of a ZW/ZZ sex chromosomal system in the species.

Bottom Line: Differential gene expression among three varieties provides insights into the genetic basis of colour variation.A potential heterogametic sex chromosome is identified in the female arowana karyotype, suggesting that the sex is determined by a ZW/ZZ sex chromosomal system.The high-quality reference genome of the golden arowana and the draft assemblies of the red and green varieties are valuable resources for understanding the biology, adaptation and behaviour of Asian arowanas.

View Article: PubMed Central - PubMed

Affiliation: Shenzhen Key Lab of Marine Genomics, Guangdong Provincial Key Lab of Molecular Breeding in Marine Economic Animals, Shenzhen 518083, China.

ABSTRACT
The Asian arowana (Scleropages formosus), one of the world's most expensive cultivated ornamental fishes, is an endangered species. It represents an ancient lineage of teleosts: the Osteoglossomorpha. Here, we provide a high-quality chromosome-level reference genome of a female golden-variety arowana using a combination of deep shotgun sequencing and high-resolution linkage mapping. In addition, we have also generated two draft genome assemblies for the red and green varieties. Phylogenomic analysis supports a sister group relationship between Osteoglossomorpha (bonytongues) and Elopomorpha (eels and relatives), with the two clades together forming a sister group of Clupeocephala which includes all the remaining teleosts. The arowana genome retains the full complement of eight Hox clusters unlike the African butterfly fish (Pantodon buchholzi), another bonytongue fish, which possess only five Hox clusters. Differential gene expression among three varieties provides insights into the genetic basis of colour variation. A potential heterogametic sex chromosome is identified in the female arowana karyotype, suggesting that the sex is determined by a ZW/ZZ sex chromosomal system. The high-quality reference genome of the golden arowana and the draft assemblies of the red and green varieties are valuable resources for understanding the biology, adaptation and behaviour of Asian arowanas.

No MeSH data available.


Related in: MedlinePlus