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Dual-functional transdermal drug delivery system with controllable drug loading based on thermosensitive poloxamer hydrogel for atopic dermatitis treatment.

Wang W, Wat E, Hui PC, Chan B, Ng FS, Kan CW, Wang X, Hu H, Wong EC, Lau CB, Leung PC - Sci Rep (2016)

Bottom Line: The treatment of atopic dermatitis (AD) has long been viewed as a problematic issue by the medical profession.It was found that the presence of CMCs can appreciably improve the physical properties of P407 hydrogel, which makes it more suitable for tailored drug loading.The fabricated P407/CMCs composite hydrogel was also characterized in terms of surface morphology by field emission scanning electron microscopy (FE-SEM), rheological properties by a rheometer, release profile in vitro by dialysis method and cytotoxicity test.

View Article: PubMed Central - PubMed

Affiliation: Institute of Textiles and Clothing, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong, China.

ABSTRACT
The treatment of atopic dermatitis (AD) has long been viewed as a problematic issue by the medical profession. Although a wide variety of complementary therapies have been introduced, they fail to combine the skin moisturizing and drug supply for AD patients. This study reports the development of a thermo-sensitive Poloxamer 407/Carboxymethyl cellulose sodium (P407/CMCs) composite hydrogel formulation with twin functions of moisture and drug supply for AD treatment. It was found that the presence of CMCs can appreciably improve the physical properties of P407 hydrogel, which makes it more suitable for tailored drug loading. The fabricated P407/CMCs composite hydrogel was also characterized in terms of surface morphology by field emission scanning electron microscopy (FE-SEM), rheological properties by a rheometer, release profile in vitro by dialysis method and cytotoxicity test. More importantly, the findings from transdermal drug delivery behavior revealed that P407/CMCs showed desirable percutaneous performance. Additionally, analysis of cytotoxicity test suggested that P407/CMCs composite hydrogel is a high-security therapy for clinical trials and thus exhibits a promising way to treat AD with skin moisturizing and medication.

No MeSH data available.


Related in: MedlinePlus

Cumulative release of GA permeated through porcine ear skin (n = 3, mean values ± SD).CM-1 representing CM solution, CM-2 representing CM solution with 4% (w/w) CMCS, GA-1 representing GA solution and GA-2 representing GA solution with 4% (w/w) CMCS, concentrations of CM and GA were identical to PC200, PC202 and PC204.
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f9: Cumulative release of GA permeated through porcine ear skin (n = 3, mean values ± SD).CM-1 representing CM solution, CM-2 representing CM solution with 4% (w/w) CMCS, GA-1 representing GA solution and GA-2 representing GA solution with 4% (w/w) CMCS, concentrations of CM and GA were identical to PC200, PC202 and PC204.

Mentions: Next, the profiles of in vitro release of CM from three composite hydrogel formulations were investigated by the dialysis method (Fig. 7). Although the addition of CMCs lowered the release rate of CM due to an increase of viscosity of the system, the accumulative release percentage still reached close to 90% (89% for PC202 and 81% for PC204) after 48 h. On the contrary, however, from the transdermal analysis results (Fig. 8), it can be clearly observed that the percutaneous behavior of CM across the porcine skin was significantly enhanced in the presence of CMCs, especially after 48 h. This was further validated by the statistical analysis (p < 0.01). The reason, we speculate, may be ascribed to either the penetration enhancing effect of CMCs on GA39 or the increasing porosity of the composite hydrogel as CMCs was added2850. In transdermal drug delivery, penetration enhancers are commonly used to promote the diffusion of drug through the skin by overcoming the barrier function of stratum corneum39. To verify our speculation, an experiment on the ability of CMCs to enhance penetration of GA through the skin was conducted (Fig. 9). In the experiment, the penetration behavior of CM in the presence of CMCs was first investigated. As a control, GA was also tested in terms of percutaneous behavior in the presence of CMCs. The percutaneous analysis showed that the permeation of GA across the skin was not significantly enhanced (p > 0.05) with the aid of CMCs, both for CM and GA specimens, indicating that CMCs has no permeation enhancing ability. Hence, an increase in the porous structures of composite hydrogels with the addition of CMCs was the paramount parameter which accounted for enhancement of the penetration behavior of CM.


Dual-functional transdermal drug delivery system with controllable drug loading based on thermosensitive poloxamer hydrogel for atopic dermatitis treatment.

Wang W, Wat E, Hui PC, Chan B, Ng FS, Kan CW, Wang X, Hu H, Wong EC, Lau CB, Leung PC - Sci Rep (2016)

Cumulative release of GA permeated through porcine ear skin (n = 3, mean values ± SD).CM-1 representing CM solution, CM-2 representing CM solution with 4% (w/w) CMCS, GA-1 representing GA solution and GA-2 representing GA solution with 4% (w/w) CMCS, concentrations of CM and GA were identical to PC200, PC202 and PC204.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4835724&req=5

f9: Cumulative release of GA permeated through porcine ear skin (n = 3, mean values ± SD).CM-1 representing CM solution, CM-2 representing CM solution with 4% (w/w) CMCS, GA-1 representing GA solution and GA-2 representing GA solution with 4% (w/w) CMCS, concentrations of CM and GA were identical to PC200, PC202 and PC204.
Mentions: Next, the profiles of in vitro release of CM from three composite hydrogel formulations were investigated by the dialysis method (Fig. 7). Although the addition of CMCs lowered the release rate of CM due to an increase of viscosity of the system, the accumulative release percentage still reached close to 90% (89% for PC202 and 81% for PC204) after 48 h. On the contrary, however, from the transdermal analysis results (Fig. 8), it can be clearly observed that the percutaneous behavior of CM across the porcine skin was significantly enhanced in the presence of CMCs, especially after 48 h. This was further validated by the statistical analysis (p < 0.01). The reason, we speculate, may be ascribed to either the penetration enhancing effect of CMCs on GA39 or the increasing porosity of the composite hydrogel as CMCs was added2850. In transdermal drug delivery, penetration enhancers are commonly used to promote the diffusion of drug through the skin by overcoming the barrier function of stratum corneum39. To verify our speculation, an experiment on the ability of CMCs to enhance penetration of GA through the skin was conducted (Fig. 9). In the experiment, the penetration behavior of CM in the presence of CMCs was first investigated. As a control, GA was also tested in terms of percutaneous behavior in the presence of CMCs. The percutaneous analysis showed that the permeation of GA across the skin was not significantly enhanced (p > 0.05) with the aid of CMCs, both for CM and GA specimens, indicating that CMCs has no permeation enhancing ability. Hence, an increase in the porous structures of composite hydrogels with the addition of CMCs was the paramount parameter which accounted for enhancement of the penetration behavior of CM.

Bottom Line: The treatment of atopic dermatitis (AD) has long been viewed as a problematic issue by the medical profession.It was found that the presence of CMCs can appreciably improve the physical properties of P407 hydrogel, which makes it more suitable for tailored drug loading.The fabricated P407/CMCs composite hydrogel was also characterized in terms of surface morphology by field emission scanning electron microscopy (FE-SEM), rheological properties by a rheometer, release profile in vitro by dialysis method and cytotoxicity test.

View Article: PubMed Central - PubMed

Affiliation: Institute of Textiles and Clothing, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong, China.

ABSTRACT
The treatment of atopic dermatitis (AD) has long been viewed as a problematic issue by the medical profession. Although a wide variety of complementary therapies have been introduced, they fail to combine the skin moisturizing and drug supply for AD patients. This study reports the development of a thermo-sensitive Poloxamer 407/Carboxymethyl cellulose sodium (P407/CMCs) composite hydrogel formulation with twin functions of moisture and drug supply for AD treatment. It was found that the presence of CMCs can appreciably improve the physical properties of P407 hydrogel, which makes it more suitable for tailored drug loading. The fabricated P407/CMCs composite hydrogel was also characterized in terms of surface morphology by field emission scanning electron microscopy (FE-SEM), rheological properties by a rheometer, release profile in vitro by dialysis method and cytotoxicity test. More importantly, the findings from transdermal drug delivery behavior revealed that P407/CMCs showed desirable percutaneous performance. Additionally, analysis of cytotoxicity test suggested that P407/CMCs composite hydrogel is a high-security therapy for clinical trials and thus exhibits a promising way to treat AD with skin moisturizing and medication.

No MeSH data available.


Related in: MedlinePlus