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Global Transcriptome Profiling Analysis of Inhibitory Effects of Paclobutrazol on Leaf Growth in Lily (Lilium Longiflorum-Asiatic Hybrid).

Zhu X, Chai M, Li Y, Sun M, Zhang J, Sun G, Jiang C, Shi L - Front Plant Sci (2016)

Bottom Line: By analyzing dynamic changes of differentially expressed genes, nine metabolic pathways and signal transduction pathways were significantly enriched and many potentially interesting genes were identified that encoded putative regulators or key components of cell division, cell expansion, GA metabolism and signaling transduction and these genes were highlighted to reveal their importance in regulation of plant size.These results will provide a better understanding of the molecular mechanism on lily dwarfism and some potential genes related to lily organ size, which will lay the foundation for molecular breeding of potted lilies.These transcriptome data will also serve as valuable public genomic resources for other genetic research in lily.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Plant Resources and Beijing Botanical Garden, Institute of Botany, Chinese Academy of SciencesBeijing, China; University of Chinese Academy of SciencesBeijing, China.

ABSTRACT
As a popular ornamental flower, potted lily is an important object of lily breeding. Paclobutrazol, a chemical growth retardation compound, is often used to dwarf plant in producing potted lilies. However, in recent years, the plants with inherited dwarf traits by using genetic engineer breeding technology are being developed. The studies on molecular basis of lily dwarfism will offer some target genes which have profound dwarf effect for genetic engineer breeding. Here, we confirmed that paclobutrazol inhibited plant height and leaf size in Lilium Longiflorum-Asiatic hybrid, and then RNA-Seq technique was employed to analyze gene transcripts of Lilium Longiflorum-Asiatic hybrid leaves by paclobutrazol treatment in order to get a deeper insight into dwarfism mechanism of lily. Approximately 38.6 Gb data was obtained and assemble into 53,681 unigenes. Annotation, pathways, functional classification and phylogenetic classification of these data were analyzed based on Nr, Nt, Swiss-Prot, KEGG, COG, and GO databases. 2704 differentially expressed genes were screened by comparing paclobutrazol-treated samples with untreated samples and quantitative real-time PCR was performed to validate expression profiles. By analyzing dynamic changes of differentially expressed genes, nine metabolic pathways and signal transduction pathways were significantly enriched and many potentially interesting genes were identified that encoded putative regulators or key components of cell division, cell expansion, GA metabolism and signaling transduction and these genes were highlighted to reveal their importance in regulation of plant size. These results will provide a better understanding of the molecular mechanism on lily dwarfism and some potential genes related to lily organ size, which will lay the foundation for molecular breeding of potted lilies. These transcriptome data will also serve as valuable public genomic resources for other genetic research in lily.

No MeSH data available.


Related in: MedlinePlus

Expression profiles of DEGs involved in cell division and cell expansion in lily leaves by PBZ treatment. (A) Genes involved in cell division. (B) Genes involved in cell expansion. The bar represents the scale of the expression levels for each gene (log2FPKM) in the CK, PBZ-treated sample at 3, 24 and 72 h. Red rectangles indicate upregulation of genes, and green rectangles indicate down-regulation. Complete information for each gene list can be found in Table S4.
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Figure 8: Expression profiles of DEGs involved in cell division and cell expansion in lily leaves by PBZ treatment. (A) Genes involved in cell division. (B) Genes involved in cell expansion. The bar represents the scale of the expression levels for each gene (log2FPKM) in the CK, PBZ-treated sample at 3, 24 and 72 h. Red rectangles indicate upregulation of genes, and green rectangles indicate down-regulation. Complete information for each gene list can be found in Table S4.

Mentions: In this study, we observed that PBZ significantly reduced leaf size in L. LA hybrid. Leaf size is governed by cell division and cell expansion processes. Thus, the DEGs associated with cell division and cell expansion were screened. Cell division is highly regulated by sequential progression through cell cycle phases. According to the phases of the cell cycle, nine DEGs associated with cell division included three genes involved in the cell cycle (encoding CDKs), one gene involved in S phase (encoding histone H4.3), three genes involved in G2 phase (encoding DNA mismatch repair protein MSH2/MSH5/MSH7), and two genes involved in M phase (encoding kinesin) (Figure 8A). In these results, the expression levels of these genes all declined except the MSH2 gene after PBZ treatment, especially at 3 h (Figure 8A).


Global Transcriptome Profiling Analysis of Inhibitory Effects of Paclobutrazol on Leaf Growth in Lily (Lilium Longiflorum-Asiatic Hybrid).

Zhu X, Chai M, Li Y, Sun M, Zhang J, Sun G, Jiang C, Shi L - Front Plant Sci (2016)

Expression profiles of DEGs involved in cell division and cell expansion in lily leaves by PBZ treatment. (A) Genes involved in cell division. (B) Genes involved in cell expansion. The bar represents the scale of the expression levels for each gene (log2FPKM) in the CK, PBZ-treated sample at 3, 24 and 72 h. Red rectangles indicate upregulation of genes, and green rectangles indicate down-regulation. Complete information for each gene list can be found in Table S4.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4835717&req=5

Figure 8: Expression profiles of DEGs involved in cell division and cell expansion in lily leaves by PBZ treatment. (A) Genes involved in cell division. (B) Genes involved in cell expansion. The bar represents the scale of the expression levels for each gene (log2FPKM) in the CK, PBZ-treated sample at 3, 24 and 72 h. Red rectangles indicate upregulation of genes, and green rectangles indicate down-regulation. Complete information for each gene list can be found in Table S4.
Mentions: In this study, we observed that PBZ significantly reduced leaf size in L. LA hybrid. Leaf size is governed by cell division and cell expansion processes. Thus, the DEGs associated with cell division and cell expansion were screened. Cell division is highly regulated by sequential progression through cell cycle phases. According to the phases of the cell cycle, nine DEGs associated with cell division included three genes involved in the cell cycle (encoding CDKs), one gene involved in S phase (encoding histone H4.3), three genes involved in G2 phase (encoding DNA mismatch repair protein MSH2/MSH5/MSH7), and two genes involved in M phase (encoding kinesin) (Figure 8A). In these results, the expression levels of these genes all declined except the MSH2 gene after PBZ treatment, especially at 3 h (Figure 8A).

Bottom Line: By analyzing dynamic changes of differentially expressed genes, nine metabolic pathways and signal transduction pathways were significantly enriched and many potentially interesting genes were identified that encoded putative regulators or key components of cell division, cell expansion, GA metabolism and signaling transduction and these genes were highlighted to reveal their importance in regulation of plant size.These results will provide a better understanding of the molecular mechanism on lily dwarfism and some potential genes related to lily organ size, which will lay the foundation for molecular breeding of potted lilies.These transcriptome data will also serve as valuable public genomic resources for other genetic research in lily.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Plant Resources and Beijing Botanical Garden, Institute of Botany, Chinese Academy of SciencesBeijing, China; University of Chinese Academy of SciencesBeijing, China.

ABSTRACT
As a popular ornamental flower, potted lily is an important object of lily breeding. Paclobutrazol, a chemical growth retardation compound, is often used to dwarf plant in producing potted lilies. However, in recent years, the plants with inherited dwarf traits by using genetic engineer breeding technology are being developed. The studies on molecular basis of lily dwarfism will offer some target genes which have profound dwarf effect for genetic engineer breeding. Here, we confirmed that paclobutrazol inhibited plant height and leaf size in Lilium Longiflorum-Asiatic hybrid, and then RNA-Seq technique was employed to analyze gene transcripts of Lilium Longiflorum-Asiatic hybrid leaves by paclobutrazol treatment in order to get a deeper insight into dwarfism mechanism of lily. Approximately 38.6 Gb data was obtained and assemble into 53,681 unigenes. Annotation, pathways, functional classification and phylogenetic classification of these data were analyzed based on Nr, Nt, Swiss-Prot, KEGG, COG, and GO databases. 2704 differentially expressed genes were screened by comparing paclobutrazol-treated samples with untreated samples and quantitative real-time PCR was performed to validate expression profiles. By analyzing dynamic changes of differentially expressed genes, nine metabolic pathways and signal transduction pathways were significantly enriched and many potentially interesting genes were identified that encoded putative regulators or key components of cell division, cell expansion, GA metabolism and signaling transduction and these genes were highlighted to reveal their importance in regulation of plant size. These results will provide a better understanding of the molecular mechanism on lily dwarfism and some potential genes related to lily organ size, which will lay the foundation for molecular breeding of potted lilies. These transcriptome data will also serve as valuable public genomic resources for other genetic research in lily.

No MeSH data available.


Related in: MedlinePlus