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Global Transcriptome Profiling Analysis of Inhibitory Effects of Paclobutrazol on Leaf Growth in Lily (Lilium Longiflorum-Asiatic Hybrid).

Zhu X, Chai M, Li Y, Sun M, Zhang J, Sun G, Jiang C, Shi L - Front Plant Sci (2016)

Bottom Line: By analyzing dynamic changes of differentially expressed genes, nine metabolic pathways and signal transduction pathways were significantly enriched and many potentially interesting genes were identified that encoded putative regulators or key components of cell division, cell expansion, GA metabolism and signaling transduction and these genes were highlighted to reveal their importance in regulation of plant size.These results will provide a better understanding of the molecular mechanism on lily dwarfism and some potential genes related to lily organ size, which will lay the foundation for molecular breeding of potted lilies.These transcriptome data will also serve as valuable public genomic resources for other genetic research in lily.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Plant Resources and Beijing Botanical Garden, Institute of Botany, Chinese Academy of SciencesBeijing, China; University of Chinese Academy of SciencesBeijing, China.

ABSTRACT
As a popular ornamental flower, potted lily is an important object of lily breeding. Paclobutrazol, a chemical growth retardation compound, is often used to dwarf plant in producing potted lilies. However, in recent years, the plants with inherited dwarf traits by using genetic engineer breeding technology are being developed. The studies on molecular basis of lily dwarfism will offer some target genes which have profound dwarf effect for genetic engineer breeding. Here, we confirmed that paclobutrazol inhibited plant height and leaf size in Lilium Longiflorum-Asiatic hybrid, and then RNA-Seq technique was employed to analyze gene transcripts of Lilium Longiflorum-Asiatic hybrid leaves by paclobutrazol treatment in order to get a deeper insight into dwarfism mechanism of lily. Approximately 38.6 Gb data was obtained and assemble into 53,681 unigenes. Annotation, pathways, functional classification and phylogenetic classification of these data were analyzed based on Nr, Nt, Swiss-Prot, KEGG, COG, and GO databases. 2704 differentially expressed genes were screened by comparing paclobutrazol-treated samples with untreated samples and quantitative real-time PCR was performed to validate expression profiles. By analyzing dynamic changes of differentially expressed genes, nine metabolic pathways and signal transduction pathways were significantly enriched and many potentially interesting genes were identified that encoded putative regulators or key components of cell division, cell expansion, GA metabolism and signaling transduction and these genes were highlighted to reveal their importance in regulation of plant size. These results will provide a better understanding of the molecular mechanism on lily dwarfism and some potential genes related to lily organ size, which will lay the foundation for molecular breeding of potted lilies. These transcriptome data will also serve as valuable public genomic resources for other genetic research in lily.

No MeSH data available.


Related in: MedlinePlus

Morphological analysis of PBZ-treated leaves and leaf cell compared with controls (CK). (A) Leaf length, leaf width and leaf area measurements. Values are presented as the mean ± standard deviation (SD) of five samples in each group. Values sharing different superscript letters differ significantly (P ≤ 0.05). (B) Photograph of the individual leaves from PBZ-treated samples and CK. (C,D) Morphology of epidermal cells from the middle area of a half leaf next to the midvein by SEM; C, controls; D, PBZ-treated; the scale is 200 μm.
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Figure 2: Morphological analysis of PBZ-treated leaves and leaf cell compared with controls (CK). (A) Leaf length, leaf width and leaf area measurements. Values are presented as the mean ± standard deviation (SD) of five samples in each group. Values sharing different superscript letters differ significantly (P ≤ 0.05). (B) Photograph of the individual leaves from PBZ-treated samples and CK. (C,D) Morphology of epidermal cells from the middle area of a half leaf next to the midvein by SEM; C, controls; D, PBZ-treated; the scale is 200 μm.

Mentions: Plant height was shorter for all PBZ-treated plants compared with control plants and was suppressed more and more severely as PBZ concentration increased (Figure 1). To study acting mechanism of PBZ on lily, the most severely dwarf group applied by 500 mg L−1 PBZ solution and the control group were used as following research. The leaf shape between the two groups was firstly observed. The leaf length, leaf width and leaf area were measured. The results showed that PBZ significantly decreased leaf area and leaf length but had no effect on leaf width in lily (Figures 2A,B). We further observed the upper epidermal cells of leaves and found that the cell length of lily leaves was decreased but cell width was not affected by PBZ treatment (Figures 2C,D). These results indicated that PBZ may inhibit leaf length by decreasing cell length in lily.


Global Transcriptome Profiling Analysis of Inhibitory Effects of Paclobutrazol on Leaf Growth in Lily (Lilium Longiflorum-Asiatic Hybrid).

Zhu X, Chai M, Li Y, Sun M, Zhang J, Sun G, Jiang C, Shi L - Front Plant Sci (2016)

Morphological analysis of PBZ-treated leaves and leaf cell compared with controls (CK). (A) Leaf length, leaf width and leaf area measurements. Values are presented as the mean ± standard deviation (SD) of five samples in each group. Values sharing different superscript letters differ significantly (P ≤ 0.05). (B) Photograph of the individual leaves from PBZ-treated samples and CK. (C,D) Morphology of epidermal cells from the middle area of a half leaf next to the midvein by SEM; C, controls; D, PBZ-treated; the scale is 200 μm.
© Copyright Policy
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4835717&req=5

Figure 2: Morphological analysis of PBZ-treated leaves and leaf cell compared with controls (CK). (A) Leaf length, leaf width and leaf area measurements. Values are presented as the mean ± standard deviation (SD) of five samples in each group. Values sharing different superscript letters differ significantly (P ≤ 0.05). (B) Photograph of the individual leaves from PBZ-treated samples and CK. (C,D) Morphology of epidermal cells from the middle area of a half leaf next to the midvein by SEM; C, controls; D, PBZ-treated; the scale is 200 μm.
Mentions: Plant height was shorter for all PBZ-treated plants compared with control plants and was suppressed more and more severely as PBZ concentration increased (Figure 1). To study acting mechanism of PBZ on lily, the most severely dwarf group applied by 500 mg L−1 PBZ solution and the control group were used as following research. The leaf shape between the two groups was firstly observed. The leaf length, leaf width and leaf area were measured. The results showed that PBZ significantly decreased leaf area and leaf length but had no effect on leaf width in lily (Figures 2A,B). We further observed the upper epidermal cells of leaves and found that the cell length of lily leaves was decreased but cell width was not affected by PBZ treatment (Figures 2C,D). These results indicated that PBZ may inhibit leaf length by decreasing cell length in lily.

Bottom Line: By analyzing dynamic changes of differentially expressed genes, nine metabolic pathways and signal transduction pathways were significantly enriched and many potentially interesting genes were identified that encoded putative regulators or key components of cell division, cell expansion, GA metabolism and signaling transduction and these genes were highlighted to reveal their importance in regulation of plant size.These results will provide a better understanding of the molecular mechanism on lily dwarfism and some potential genes related to lily organ size, which will lay the foundation for molecular breeding of potted lilies.These transcriptome data will also serve as valuable public genomic resources for other genetic research in lily.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Plant Resources and Beijing Botanical Garden, Institute of Botany, Chinese Academy of SciencesBeijing, China; University of Chinese Academy of SciencesBeijing, China.

ABSTRACT
As a popular ornamental flower, potted lily is an important object of lily breeding. Paclobutrazol, a chemical growth retardation compound, is often used to dwarf plant in producing potted lilies. However, in recent years, the plants with inherited dwarf traits by using genetic engineer breeding technology are being developed. The studies on molecular basis of lily dwarfism will offer some target genes which have profound dwarf effect for genetic engineer breeding. Here, we confirmed that paclobutrazol inhibited plant height and leaf size in Lilium Longiflorum-Asiatic hybrid, and then RNA-Seq technique was employed to analyze gene transcripts of Lilium Longiflorum-Asiatic hybrid leaves by paclobutrazol treatment in order to get a deeper insight into dwarfism mechanism of lily. Approximately 38.6 Gb data was obtained and assemble into 53,681 unigenes. Annotation, pathways, functional classification and phylogenetic classification of these data were analyzed based on Nr, Nt, Swiss-Prot, KEGG, COG, and GO databases. 2704 differentially expressed genes were screened by comparing paclobutrazol-treated samples with untreated samples and quantitative real-time PCR was performed to validate expression profiles. By analyzing dynamic changes of differentially expressed genes, nine metabolic pathways and signal transduction pathways were significantly enriched and many potentially interesting genes were identified that encoded putative regulators or key components of cell division, cell expansion, GA metabolism and signaling transduction and these genes were highlighted to reveal their importance in regulation of plant size. These results will provide a better understanding of the molecular mechanism on lily dwarfism and some potential genes related to lily organ size, which will lay the foundation for molecular breeding of potted lilies. These transcriptome data will also serve as valuable public genomic resources for other genetic research in lily.

No MeSH data available.


Related in: MedlinePlus