Limits...
Human Dental Pulp Stem Cells and Gingival Fibroblasts Seeded into Silk Fibroin Scaffolds Have the Same Ability in Attracting Vessels.

Woloszyk A, Buschmann J, Waschkies C, Stadlinger B, Mitsiadis TA - Front Physiol (2016)

Bottom Line: Therefore, here we studied the attraction, ingrowth, and distribution of blood vessels from the chicken embryo chorioallantoic membrane into implanted silk fibroin scaffolds seeded with either human dental pulp stem cells or human gingival fibroblasts.Our results demonstrate that human dental pulp stem cells and gingival fibroblasts possess equal abilities in attracting vessels within silk fibroin scaffolds.Additionally, the prolonged in vitro pre-incubation period of these two cell populations favors the homogeneous distribution of vessels within silk fibroin scaffolds, which further improves implant survival and guarantees successful healing and regeneration.

View Article: PubMed Central - PubMed

Affiliation: Orofacial Development and Regeneration, Center of Dental Medicine, Institute of Oral Biology, University of Zurich Zurich, Switzerland.

ABSTRACT
Neovascularization is one of the most important processes during tissue repair and regeneration. Current healing approaches based on the use of biomaterials combined with stem cells in critical-size bone defects fail due to the insufficient implant vascularization and integration into the host tissues. Therefore, here we studied the attraction, ingrowth, and distribution of blood vessels from the chicken embryo chorioallantoic membrane into implanted silk fibroin scaffolds seeded with either human dental pulp stem cells or human gingival fibroblasts. Perfusion capacity was evaluated by non-invasive in vivo Magnetic Resonance Imaging while the number and density of blood vessels were measured by histomorphometry. Our results demonstrate that human dental pulp stem cells and gingival fibroblasts possess equal abilities in attracting vessels within silk fibroin scaffolds. Additionally, the prolonged in vitro pre-incubation period of these two cell populations favors the homogeneous distribution of vessels within silk fibroin scaffolds, which further improves implant survival and guarantees successful healing and regeneration.

No MeSH data available.


Related in: MedlinePlus

Hematoxylin and Eosin stainings of longitudinal sections and histomorphometric analysis. (A1, 3, 5, 7) hDPSCs-seeded scaffolds. (A2, 4, 6, 8) hGFs-seeded scaffolds. (A1, 2) Scaffold section overview. Blue dashed line indicates the front line of the growing tissue. Red dotted line indicates the outline of the scaffold. Scale bar = 1 mm. (A3, 4) Magnifications showing vascularization in the area marked with a red box in the corresponding overview picture. Arrows indicate capillaries. Scale bar = 25 μm. (A5, 6) Magnifications showing single capillaries. Scale bar = 10 μm. (A7, 8) Schematic representation of the capillaries shown in 5 and 6. (B1) Number of vessels per scaffold area. (B2) Percent vessel area per scaffold area. Values are given as mean ± standard deviation. No statistically significant differences were found. EC, endothelial cells; hDPSCs, human dental pulp stem cells; hGFs, human gingival fibroblasts.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4835714&req=5

Figure 2: Hematoxylin and Eosin stainings of longitudinal sections and histomorphometric analysis. (A1, 3, 5, 7) hDPSCs-seeded scaffolds. (A2, 4, 6, 8) hGFs-seeded scaffolds. (A1, 2) Scaffold section overview. Blue dashed line indicates the front line of the growing tissue. Red dotted line indicates the outline of the scaffold. Scale bar = 1 mm. (A3, 4) Magnifications showing vascularization in the area marked with a red box in the corresponding overview picture. Arrows indicate capillaries. Scale bar = 25 μm. (A5, 6) Magnifications showing single capillaries. Scale bar = 10 μm. (A7, 8) Schematic representation of the capillaries shown in 5 and 6. (B1) Number of vessels per scaffold area. (B2) Percent vessel area per scaffold area. Values are given as mean ± standard deviation. No statistically significant differences were found. EC, endothelial cells; hDPSCs, human dental pulp stem cells; hGFs, human gingival fibroblasts.

Mentions: Qualitative and quantitative analyses of the vascularized scaffolds were performed using sections stained with Hematoxylin and Eosin (H&E). These histological sections showed large areas of tissue expansion (depicted by the blue dashed line) into the scaffold (depicted by the red dotted line) (Figure 2A). While in the samples seeded with hDPSCs the growing tissue filled approximately half of the scaffold areas (Figure 2A1), the tissue occupied roughly two-thirds of the scaffolds seeded with hGFs (Figure 2A2). CAM-derived blood vessels have penetrated both hDPSC- and hGF-seeded scaffolds (Figures 2A3–6). A manual analysis was performed in order to determine the number and the percent area occupied by the vessels within the three defined scaffold areas (Figure 2B). The corresponding graphs showed proportional values for scaffolds seeded with hDPSCs and hGFs. Although the average size of the counted vessels in both scaffolds was the same, a higher number of vessels was counted in hGFs-seeded scaffolds. This is due to the larger area occupied by the tissue in hGFs-seeded scaffolds when compared to the occupied tissue area in hDPSC-seeded scaffolds. The distribution of the vasculature along the horizontal axis of the scaffold was nearly homogeneous, whereas the vessel density decreased along the vertical axis following an interface-middle-surface gradient.


Human Dental Pulp Stem Cells and Gingival Fibroblasts Seeded into Silk Fibroin Scaffolds Have the Same Ability in Attracting Vessels.

Woloszyk A, Buschmann J, Waschkies C, Stadlinger B, Mitsiadis TA - Front Physiol (2016)

Hematoxylin and Eosin stainings of longitudinal sections and histomorphometric analysis. (A1, 3, 5, 7) hDPSCs-seeded scaffolds. (A2, 4, 6, 8) hGFs-seeded scaffolds. (A1, 2) Scaffold section overview. Blue dashed line indicates the front line of the growing tissue. Red dotted line indicates the outline of the scaffold. Scale bar = 1 mm. (A3, 4) Magnifications showing vascularization in the area marked with a red box in the corresponding overview picture. Arrows indicate capillaries. Scale bar = 25 μm. (A5, 6) Magnifications showing single capillaries. Scale bar = 10 μm. (A7, 8) Schematic representation of the capillaries shown in 5 and 6. (B1) Number of vessels per scaffold area. (B2) Percent vessel area per scaffold area. Values are given as mean ± standard deviation. No statistically significant differences were found. EC, endothelial cells; hDPSCs, human dental pulp stem cells; hGFs, human gingival fibroblasts.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4835714&req=5

Figure 2: Hematoxylin and Eosin stainings of longitudinal sections and histomorphometric analysis. (A1, 3, 5, 7) hDPSCs-seeded scaffolds. (A2, 4, 6, 8) hGFs-seeded scaffolds. (A1, 2) Scaffold section overview. Blue dashed line indicates the front line of the growing tissue. Red dotted line indicates the outline of the scaffold. Scale bar = 1 mm. (A3, 4) Magnifications showing vascularization in the area marked with a red box in the corresponding overview picture. Arrows indicate capillaries. Scale bar = 25 μm. (A5, 6) Magnifications showing single capillaries. Scale bar = 10 μm. (A7, 8) Schematic representation of the capillaries shown in 5 and 6. (B1) Number of vessels per scaffold area. (B2) Percent vessel area per scaffold area. Values are given as mean ± standard deviation. No statistically significant differences were found. EC, endothelial cells; hDPSCs, human dental pulp stem cells; hGFs, human gingival fibroblasts.
Mentions: Qualitative and quantitative analyses of the vascularized scaffolds were performed using sections stained with Hematoxylin and Eosin (H&E). These histological sections showed large areas of tissue expansion (depicted by the blue dashed line) into the scaffold (depicted by the red dotted line) (Figure 2A). While in the samples seeded with hDPSCs the growing tissue filled approximately half of the scaffold areas (Figure 2A1), the tissue occupied roughly two-thirds of the scaffolds seeded with hGFs (Figure 2A2). CAM-derived blood vessels have penetrated both hDPSC- and hGF-seeded scaffolds (Figures 2A3–6). A manual analysis was performed in order to determine the number and the percent area occupied by the vessels within the three defined scaffold areas (Figure 2B). The corresponding graphs showed proportional values for scaffolds seeded with hDPSCs and hGFs. Although the average size of the counted vessels in both scaffolds was the same, a higher number of vessels was counted in hGFs-seeded scaffolds. This is due to the larger area occupied by the tissue in hGFs-seeded scaffolds when compared to the occupied tissue area in hDPSC-seeded scaffolds. The distribution of the vasculature along the horizontal axis of the scaffold was nearly homogeneous, whereas the vessel density decreased along the vertical axis following an interface-middle-surface gradient.

Bottom Line: Therefore, here we studied the attraction, ingrowth, and distribution of blood vessels from the chicken embryo chorioallantoic membrane into implanted silk fibroin scaffolds seeded with either human dental pulp stem cells or human gingival fibroblasts.Our results demonstrate that human dental pulp stem cells and gingival fibroblasts possess equal abilities in attracting vessels within silk fibroin scaffolds.Additionally, the prolonged in vitro pre-incubation period of these two cell populations favors the homogeneous distribution of vessels within silk fibroin scaffolds, which further improves implant survival and guarantees successful healing and regeneration.

View Article: PubMed Central - PubMed

Affiliation: Orofacial Development and Regeneration, Center of Dental Medicine, Institute of Oral Biology, University of Zurich Zurich, Switzerland.

ABSTRACT
Neovascularization is one of the most important processes during tissue repair and regeneration. Current healing approaches based on the use of biomaterials combined with stem cells in critical-size bone defects fail due to the insufficient implant vascularization and integration into the host tissues. Therefore, here we studied the attraction, ingrowth, and distribution of blood vessels from the chicken embryo chorioallantoic membrane into implanted silk fibroin scaffolds seeded with either human dental pulp stem cells or human gingival fibroblasts. Perfusion capacity was evaluated by non-invasive in vivo Magnetic Resonance Imaging while the number and density of blood vessels were measured by histomorphometry. Our results demonstrate that human dental pulp stem cells and gingival fibroblasts possess equal abilities in attracting vessels within silk fibroin scaffolds. Additionally, the prolonged in vitro pre-incubation period of these two cell populations favors the homogeneous distribution of vessels within silk fibroin scaffolds, which further improves implant survival and guarantees successful healing and regeneration.

No MeSH data available.


Related in: MedlinePlus