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Smaller Absolute Quantities but Greater Relative Densities of Microvessels Are Associated with Cerebellar Degeneration in Lurcher Mice.

Kolinko Y, Cendelin J, Kralickova M, Tonar Z - Front Neuroanat (2016)

Bottom Line: The greatest number and length of vessels were found in the granular layer; the number of vessels was lower in the molecular layer, and the lowest number of vessels was observed in the cerebellar nuclei corresponding with their low volume.Nevertheless, the nuclei had the greatest density of blood vessels.The complete primary morphometric data, in the form of continuous variables, is included as a supplement.

View Article: PubMed Central - PubMed

Affiliation: Biomedical Center, Faculty of Medicine in Pilsen, Charles University in Prague Pilsen, Czech Republic.

ABSTRACT
Degenerative affections of nerve tissues are often accompanied by changes of vascularization. In this regard, not much is known about hereditary cerebellar degeneration. In this study, we compared the vascularity of the individual cerebellar components and the mesencephalon of 3-month-old wild type mice (n = 5) and Lurcher mutant mice, which represent a model of hereditary olivocerebellar degeneration (n = 5). Paraformaldehyde-fixed brains were processed into 18-μm thick serial sections with random orientation. Microvessels were visualized using polyclonal rabbit anti-laminin antibodies. Then, the stacks comprised of three 5-μm thick optical sections were recorded using systematic uniform random sampling. Stereological assessment was conducted based on photo-documentation. We found that each of the cerebellar components has its own features of vascularity. The greatest number and length of vessels were found in the granular layer; the number of vessels was lower in the molecular layer, and the lowest number of vessels was observed in the cerebellar nuclei corresponding with their low volume. Nevertheless, the nuclei had the greatest density of blood vessels. The reduction of cerebellum volume in the Lurcher mice was accompanied by a reduction in vascularization in the individual cerebellar components, mainly in the cortex. Moreover, despite the lower density of microvessels in the Lurcher mice compared with the wild type mice, the relative density of microvessels in the cerebellar cortex and nuclei was greater in Lurcher mice. The complete primary morphometric data, in the form of continuous variables, is included as a supplement. Mapping of the cerebellar and midbrain microvessels has explanatory potential for studies using mouse models of neurodegeneration.

No MeSH data available.


Related in: MedlinePlus

Comparing quantitative parameters of microvessels between wild type (WT mice) and Lurcher mice. (A)—Relative proportions of the individual components of the cerebellum; (B)—Mean length of vessels in the cerebellum, its individual components and in the midbrain. Corresponding anatomical compartments were compared using the Mann-Whitney U test (significant results are presented within the diagrams: *p < 0.05, **p < 0.01).
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Figure 3: Comparing quantitative parameters of microvessels between wild type (WT mice) and Lurcher mice. (A)—Relative proportions of the individual components of the cerebellum; (B)—Mean length of vessels in the cerebellum, its individual components and in the midbrain. Corresponding anatomical compartments were compared using the Mann-Whitney U test (significant results are presented within the diagrams: *p < 0.05, **p < 0.01).

Mentions: The volume of the cerebellum, as well as its individual parts, and the volume of the midbrain are shown on Figure 2A. The relative proportions of the individual components of the cerebellum are shown in Figure 3A. The volume of the cerebellum in WT mice exceeded the volume of the midbrain. The largest share of the volume of the cerebellum belonged to the cortex and was evenly distributed between the molecular and granular layers (p = 0.68). Cerebellar nuclei were the smallest part of the cerebellum and only made up 3% of its total volume. The CE of the total volume of the cerebellum was 0.02 ± 0.003% (mean ± standard deviation).


Smaller Absolute Quantities but Greater Relative Densities of Microvessels Are Associated with Cerebellar Degeneration in Lurcher Mice.

Kolinko Y, Cendelin J, Kralickova M, Tonar Z - Front Neuroanat (2016)

Comparing quantitative parameters of microvessels between wild type (WT mice) and Lurcher mice. (A)—Relative proportions of the individual components of the cerebellum; (B)—Mean length of vessels in the cerebellum, its individual components and in the midbrain. Corresponding anatomical compartments were compared using the Mann-Whitney U test (significant results are presented within the diagrams: *p < 0.05, **p < 0.01).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4835681&req=5

Figure 3: Comparing quantitative parameters of microvessels between wild type (WT mice) and Lurcher mice. (A)—Relative proportions of the individual components of the cerebellum; (B)—Mean length of vessels in the cerebellum, its individual components and in the midbrain. Corresponding anatomical compartments were compared using the Mann-Whitney U test (significant results are presented within the diagrams: *p < 0.05, **p < 0.01).
Mentions: The volume of the cerebellum, as well as its individual parts, and the volume of the midbrain are shown on Figure 2A. The relative proportions of the individual components of the cerebellum are shown in Figure 3A. The volume of the cerebellum in WT mice exceeded the volume of the midbrain. The largest share of the volume of the cerebellum belonged to the cortex and was evenly distributed between the molecular and granular layers (p = 0.68). Cerebellar nuclei were the smallest part of the cerebellum and only made up 3% of its total volume. The CE of the total volume of the cerebellum was 0.02 ± 0.003% (mean ± standard deviation).

Bottom Line: The greatest number and length of vessels were found in the granular layer; the number of vessels was lower in the molecular layer, and the lowest number of vessels was observed in the cerebellar nuclei corresponding with their low volume.Nevertheless, the nuclei had the greatest density of blood vessels.The complete primary morphometric data, in the form of continuous variables, is included as a supplement.

View Article: PubMed Central - PubMed

Affiliation: Biomedical Center, Faculty of Medicine in Pilsen, Charles University in Prague Pilsen, Czech Republic.

ABSTRACT
Degenerative affections of nerve tissues are often accompanied by changes of vascularization. In this regard, not much is known about hereditary cerebellar degeneration. In this study, we compared the vascularity of the individual cerebellar components and the mesencephalon of 3-month-old wild type mice (n = 5) and Lurcher mutant mice, which represent a model of hereditary olivocerebellar degeneration (n = 5). Paraformaldehyde-fixed brains were processed into 18-μm thick serial sections with random orientation. Microvessels were visualized using polyclonal rabbit anti-laminin antibodies. Then, the stacks comprised of three 5-μm thick optical sections were recorded using systematic uniform random sampling. Stereological assessment was conducted based on photo-documentation. We found that each of the cerebellar components has its own features of vascularity. The greatest number and length of vessels were found in the granular layer; the number of vessels was lower in the molecular layer, and the lowest number of vessels was observed in the cerebellar nuclei corresponding with their low volume. Nevertheless, the nuclei had the greatest density of blood vessels. The reduction of cerebellum volume in the Lurcher mice was accompanied by a reduction in vascularization in the individual cerebellar components, mainly in the cortex. Moreover, despite the lower density of microvessels in the Lurcher mice compared with the wild type mice, the relative density of microvessels in the cerebellar cortex and nuclei was greater in Lurcher mice. The complete primary morphometric data, in the form of continuous variables, is included as a supplement. Mapping of the cerebellar and midbrain microvessels has explanatory potential for studies using mouse models of neurodegeneration.

No MeSH data available.


Related in: MedlinePlus