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Phytochemical Composition, Antifungal and Antioxidant Activity of Duguetia furfuracea A. St.-Hill.

Pinho FV, da Cruz LC, Rodrigues NR, Waczuk EP, Souza CE, Coutinho HD, da Costa JG, Athayde ML, Boligon AA, Franco JL, Posser T, de Menezes IR - Oxid Med Cell Longev (2016)

Bottom Line: Conclusion.The highest levels of phenols and total flavonoids were marked with antioxidant effect.This is the first report of bioactivity of the synergic effect of HEDF and fractions.

View Article: PubMed Central - PubMed

Affiliation: Department of Biological Chemistry, Laboratory of Pharmacology and Molecular Chemistry (LFQM), Regional University of Cariri (URCA), 63100-000 Crato, CE, Brazil; Department of Biochemistry and Molecular Biology, Postgraduate Program in Toxicological Biochemistry, Federal University of Santa Maria (UFSM), 97105-900 Santa Maria, RS, Brazil.

ABSTRACT

Unlabelled: Background. Duguetia furfuracea is popular plant used in popular medicine. Hypothesis/Purpose. This claim evaluated the phytochemical composition of the hydroethanolic extract (HEDF), fractions of Duguetia furfuracea, and antioxidant and antifungal activity. Methods. The chemical profile was carried out by HPLC-DAD. The total phenolic contents and flavonoid components were determined by Folin-Ciocalteu and aluminium chloride reaction. The antioxidant activity was measured by scavenging of 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical and ferric reducing ability of plasma (FRAP) methods. The antifungal activity was determined by microdilution assay.

Results: HPLC analysis revealed caffeic acid and rutin as major compounds (HEDF), caffeic acid and quercitrin (Mt-OH fraction), and quercitrin and isoquercitrin (Ac-OEt fraction). The highest levels of phenols and total flavonoids were found for Ac-OEt fraction, and the crude extract showed higher in vitro antioxidant potential. The antifungal activity showed synergic effect with fluconazole and EHDF against C. krusei, fluconazole and Mt-OH against C. krusei and C. tropicalis, and Ac-OE and fluconazole against C. albicans. Conclusion. The highest levels of phenols and total flavonoids were marked with antioxidant effect. This is the first report of bioactivity of the synergic effect of HEDF and fractions. More studies would be required to better clarify its mechanism of synergic action.

No MeSH data available.


Related in: MedlinePlus

Modulatory activity of (a) HEDF (hydroalcoholic extract of D. furfuracea), Mt-OH, and Ac-OET against the fungi C. albicans, C. krusei, and C. tropicalis (concentrations ranging from 1024 μg/mL to 0.5 μg/mL). P < 0.001 related to control group; (b) Mt-OH (methanolic extract of D. furfuracea) against the fungi C. albicans, C. krusei, and C. tropicalis (concentrations ranging from 1024 μg/mL to 0.5 μg/mL); (c) Ac-OEt (ethyl acetate extract of D. furfuracea) against the fungi C. albicans, C. krusei, and C. tropicalis (concentrations ranging from 1024 μg/mL to 0.5 μg/mL). Statistical analysis: one-way ANOVA followed by Student-Newman-Keuls test. ∗∗∗P < 0.001 versus fluconazole.
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fig2: Modulatory activity of (a) HEDF (hydroalcoholic extract of D. furfuracea), Mt-OH, and Ac-OET against the fungi C. albicans, C. krusei, and C. tropicalis (concentrations ranging from 1024 μg/mL to 0.5 μg/mL). P < 0.001 related to control group; (b) Mt-OH (methanolic extract of D. furfuracea) against the fungi C. albicans, C. krusei, and C. tropicalis (concentrations ranging from 1024 μg/mL to 0.5 μg/mL); (c) Ac-OEt (ethyl acetate extract of D. furfuracea) against the fungi C. albicans, C. krusei, and C. tropicalis (concentrations ranging from 1024 μg/mL to 0.5 μg/mL). Statistical analysis: one-way ANOVA followed by Student-Newman-Keuls test. ∗∗∗P < 0.001 versus fluconazole.

Mentions: In this study, the antifungal potential of the hydroalcoholic extract of D. furfuracea (HEDF) and methanolic (Mt-OH) and ethyl acetate (Ac-OEt) fractions was tested against standard strains of C. albicans, C. tropicalis, and C. krusei. According to the results, both extract and fractions presented minimal inhibitory concentration (CIM) ≥1024 μg/mL against all the fungi strains tested. However, the extract and the fractions of D. furfuracea presented synergic effect when they were associated with fluconazole, indicating its modulatory action against fungi when associated with clinically relevant drugs. The HEDF and Mt-OH fraction potentialized the effect of the fluconazole when tested against C. krusei as observed in Figures 2(a) and 2(b). The methanolic fraction also presented synergism with fluconazole against C. tropicalis (Figure 2(b)) and Ac-OEt fraction potentialized the effect of fluconazole against C. albicans (Figure 2(c)).


Phytochemical Composition, Antifungal and Antioxidant Activity of Duguetia furfuracea A. St.-Hill.

Pinho FV, da Cruz LC, Rodrigues NR, Waczuk EP, Souza CE, Coutinho HD, da Costa JG, Athayde ML, Boligon AA, Franco JL, Posser T, de Menezes IR - Oxid Med Cell Longev (2016)

Modulatory activity of (a) HEDF (hydroalcoholic extract of D. furfuracea), Mt-OH, and Ac-OET against the fungi C. albicans, C. krusei, and C. tropicalis (concentrations ranging from 1024 μg/mL to 0.5 μg/mL). P < 0.001 related to control group; (b) Mt-OH (methanolic extract of D. furfuracea) against the fungi C. albicans, C. krusei, and C. tropicalis (concentrations ranging from 1024 μg/mL to 0.5 μg/mL); (c) Ac-OEt (ethyl acetate extract of D. furfuracea) against the fungi C. albicans, C. krusei, and C. tropicalis (concentrations ranging from 1024 μg/mL to 0.5 μg/mL). Statistical analysis: one-way ANOVA followed by Student-Newman-Keuls test. ∗∗∗P < 0.001 versus fluconazole.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4835657&req=5

fig2: Modulatory activity of (a) HEDF (hydroalcoholic extract of D. furfuracea), Mt-OH, and Ac-OET against the fungi C. albicans, C. krusei, and C. tropicalis (concentrations ranging from 1024 μg/mL to 0.5 μg/mL). P < 0.001 related to control group; (b) Mt-OH (methanolic extract of D. furfuracea) against the fungi C. albicans, C. krusei, and C. tropicalis (concentrations ranging from 1024 μg/mL to 0.5 μg/mL); (c) Ac-OEt (ethyl acetate extract of D. furfuracea) against the fungi C. albicans, C. krusei, and C. tropicalis (concentrations ranging from 1024 μg/mL to 0.5 μg/mL). Statistical analysis: one-way ANOVA followed by Student-Newman-Keuls test. ∗∗∗P < 0.001 versus fluconazole.
Mentions: In this study, the antifungal potential of the hydroalcoholic extract of D. furfuracea (HEDF) and methanolic (Mt-OH) and ethyl acetate (Ac-OEt) fractions was tested against standard strains of C. albicans, C. tropicalis, and C. krusei. According to the results, both extract and fractions presented minimal inhibitory concentration (CIM) ≥1024 μg/mL against all the fungi strains tested. However, the extract and the fractions of D. furfuracea presented synergic effect when they were associated with fluconazole, indicating its modulatory action against fungi when associated with clinically relevant drugs. The HEDF and Mt-OH fraction potentialized the effect of the fluconazole when tested against C. krusei as observed in Figures 2(a) and 2(b). The methanolic fraction also presented synergism with fluconazole against C. tropicalis (Figure 2(b)) and Ac-OEt fraction potentialized the effect of fluconazole against C. albicans (Figure 2(c)).

Bottom Line: Conclusion.The highest levels of phenols and total flavonoids were marked with antioxidant effect.This is the first report of bioactivity of the synergic effect of HEDF and fractions.

View Article: PubMed Central - PubMed

Affiliation: Department of Biological Chemistry, Laboratory of Pharmacology and Molecular Chemistry (LFQM), Regional University of Cariri (URCA), 63100-000 Crato, CE, Brazil; Department of Biochemistry and Molecular Biology, Postgraduate Program in Toxicological Biochemistry, Federal University of Santa Maria (UFSM), 97105-900 Santa Maria, RS, Brazil.

ABSTRACT

Unlabelled: Background. Duguetia furfuracea is popular plant used in popular medicine. Hypothesis/Purpose. This claim evaluated the phytochemical composition of the hydroethanolic extract (HEDF), fractions of Duguetia furfuracea, and antioxidant and antifungal activity. Methods. The chemical profile was carried out by HPLC-DAD. The total phenolic contents and flavonoid components were determined by Folin-Ciocalteu and aluminium chloride reaction. The antioxidant activity was measured by scavenging of 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical and ferric reducing ability of plasma (FRAP) methods. The antifungal activity was determined by microdilution assay.

Results: HPLC analysis revealed caffeic acid and rutin as major compounds (HEDF), caffeic acid and quercitrin (Mt-OH fraction), and quercitrin and isoquercitrin (Ac-OEt fraction). The highest levels of phenols and total flavonoids were found for Ac-OEt fraction, and the crude extract showed higher in vitro antioxidant potential. The antifungal activity showed synergic effect with fluconazole and EHDF against C. krusei, fluconazole and Mt-OH against C. krusei and C. tropicalis, and Ac-OE and fluconazole against C. albicans. Conclusion. The highest levels of phenols and total flavonoids were marked with antioxidant effect. This is the first report of bioactivity of the synergic effect of HEDF and fractions. More studies would be required to better clarify its mechanism of synergic action.

No MeSH data available.


Related in: MedlinePlus