Limits...
Phytochemical Composition, Antifungal and Antioxidant Activity of Duguetia furfuracea A. St.-Hill.

Pinho FV, da Cruz LC, Rodrigues NR, Waczuk EP, Souza CE, Coutinho HD, da Costa JG, Athayde ML, Boligon AA, Franco JL, Posser T, de Menezes IR - Oxid Med Cell Longev (2016)

Bottom Line: Conclusion.The highest levels of phenols and total flavonoids were marked with antioxidant effect.This is the first report of bioactivity of the synergic effect of HEDF and fractions.

View Article: PubMed Central - PubMed

Affiliation: Department of Biological Chemistry, Laboratory of Pharmacology and Molecular Chemistry (LFQM), Regional University of Cariri (URCA), 63100-000 Crato, CE, Brazil; Department of Biochemistry and Molecular Biology, Postgraduate Program in Toxicological Biochemistry, Federal University of Santa Maria (UFSM), 97105-900 Santa Maria, RS, Brazil.

ABSTRACT

Unlabelled: Background. Duguetia furfuracea is popular plant used in popular medicine. Hypothesis/Purpose. This claim evaluated the phytochemical composition of the hydroethanolic extract (HEDF), fractions of Duguetia furfuracea, and antioxidant and antifungal activity. Methods. The chemical profile was carried out by HPLC-DAD. The total phenolic contents and flavonoid components were determined by Folin-Ciocalteu and aluminium chloride reaction. The antioxidant activity was measured by scavenging of 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical and ferric reducing ability of plasma (FRAP) methods. The antifungal activity was determined by microdilution assay.

Results: HPLC analysis revealed caffeic acid and rutin as major compounds (HEDF), caffeic acid and quercitrin (Mt-OH fraction), and quercitrin and isoquercitrin (Ac-OEt fraction). The highest levels of phenols and total flavonoids were found for Ac-OEt fraction, and the crude extract showed higher in vitro antioxidant potential. The antifungal activity showed synergic effect with fluconazole and EHDF against C. krusei, fluconazole and Mt-OH against C. krusei and C. tropicalis, and Ac-OE and fluconazole against C. albicans. Conclusion. The highest levels of phenols and total flavonoids were marked with antioxidant effect. This is the first report of bioactivity of the synergic effect of HEDF and fractions. More studies would be required to better clarify its mechanism of synergic action.

No MeSH data available.


Related in: MedlinePlus

Elution profiles of high performance liquid chromatography analysis: (a) HEDF (modified from [6]), (b) methanolic fraction (Mt-OH) and (c) ethyl acetate (Ac-OEt). Gallic acid (peak 1), catechin (peak 2), chlorogenic acid (peak 3), caffeic acid (peak 4), ellagic acid (peak 5), rutin (peak 6), isoquercitrin (peak 7), quercitrin (peak 8), quercetin (peak 9), and kaempferol (peak 10). Calibration curve of the gallic acid: Y = 14286x + 1395.8 (r = 0.9996); catechin: Y = 15097x + 1189.3 (r = 0.9997); caffeic acid: Y = 12758x + 1259.7 (r = 0.9996); chlorogenic acid: Y = 13461x + 1275.3 (r = 0.9992); ellagic acid: Y = 13576x + 1346.4 (r = 0.9999); rutin: Y = 12845 + 1305.7 (r = 0.9999); quercetin: Y = 13560x + 1192.6 (r = 0.9991); isoquercitrin: Y = 12873x + 1325.6 (r = 0.9998); quercitrin: Y = 11870x + 1329.8 (r = 0.9993); and kaempferol: Y = 14253x + 1238.9 (r = 0.9997).
© Copyright Policy - open-access
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4835657&req=5

fig1: Elution profiles of high performance liquid chromatography analysis: (a) HEDF (modified from [6]), (b) methanolic fraction (Mt-OH) and (c) ethyl acetate (Ac-OEt). Gallic acid (peak 1), catechin (peak 2), chlorogenic acid (peak 3), caffeic acid (peak 4), ellagic acid (peak 5), rutin (peak 6), isoquercitrin (peak 7), quercitrin (peak 8), quercetin (peak 9), and kaempferol (peak 10). Calibration curve of the gallic acid: Y = 14286x + 1395.8 (r = 0.9996); catechin: Y = 15097x + 1189.3 (r = 0.9997); caffeic acid: Y = 12758x + 1259.7 (r = 0.9996); chlorogenic acid: Y = 13461x + 1275.3 (r = 0.9992); ellagic acid: Y = 13576x + 1346.4 (r = 0.9999); rutin: Y = 12845 + 1305.7 (r = 0.9999); quercetin: Y = 13560x + 1192.6 (r = 0.9991); isoquercitrin: Y = 12873x + 1325.6 (r = 0.9998); quercitrin: Y = 11870x + 1329.8 (r = 0.9993); and kaempferol: Y = 14253x + 1238.9 (r = 0.9997).

Mentions: The main compounds present in the Mt-OH fraction were caffeic acid (32.47 ± 0.03 mg/g) and quercitrin (31.96 ± 0.03 mg/g) while catechin (2.69 ± 0.01 mg/g) and the gallic acid (5.47 ± 0.03 mg/g) were the least abundant. In the Ac-OEt fraction, major compounds were quercitrin (32.97 ± 0.037 mg/g) and isoquercitrin (31.56 ± 0.01 mg/g) while catechin (3.16 ± 0.02 mg/g) and rutin (5.49 ± 0.02 mg/g) were the least present (Figure 1 and Table 1). The chromatographic profile of HEDF demonstrated the presence of caffeic acid and rutin as major compounds (33.17 ± 0.03 mg/g and 20.05 ± 0.01 mg/g, resp.) while gallic acid (5.29 ± 0.01 mg/g) and catechin (5.31 ± 0.01 mg/g) were the least abundant [6].


Phytochemical Composition, Antifungal and Antioxidant Activity of Duguetia furfuracea A. St.-Hill.

Pinho FV, da Cruz LC, Rodrigues NR, Waczuk EP, Souza CE, Coutinho HD, da Costa JG, Athayde ML, Boligon AA, Franco JL, Posser T, de Menezes IR - Oxid Med Cell Longev (2016)

Elution profiles of high performance liquid chromatography analysis: (a) HEDF (modified from [6]), (b) methanolic fraction (Mt-OH) and (c) ethyl acetate (Ac-OEt). Gallic acid (peak 1), catechin (peak 2), chlorogenic acid (peak 3), caffeic acid (peak 4), ellagic acid (peak 5), rutin (peak 6), isoquercitrin (peak 7), quercitrin (peak 8), quercetin (peak 9), and kaempferol (peak 10). Calibration curve of the gallic acid: Y = 14286x + 1395.8 (r = 0.9996); catechin: Y = 15097x + 1189.3 (r = 0.9997); caffeic acid: Y = 12758x + 1259.7 (r = 0.9996); chlorogenic acid: Y = 13461x + 1275.3 (r = 0.9992); ellagic acid: Y = 13576x + 1346.4 (r = 0.9999); rutin: Y = 12845 + 1305.7 (r = 0.9999); quercetin: Y = 13560x + 1192.6 (r = 0.9991); isoquercitrin: Y = 12873x + 1325.6 (r = 0.9998); quercitrin: Y = 11870x + 1329.8 (r = 0.9993); and kaempferol: Y = 14253x + 1238.9 (r = 0.9997).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4835657&req=5

fig1: Elution profiles of high performance liquid chromatography analysis: (a) HEDF (modified from [6]), (b) methanolic fraction (Mt-OH) and (c) ethyl acetate (Ac-OEt). Gallic acid (peak 1), catechin (peak 2), chlorogenic acid (peak 3), caffeic acid (peak 4), ellagic acid (peak 5), rutin (peak 6), isoquercitrin (peak 7), quercitrin (peak 8), quercetin (peak 9), and kaempferol (peak 10). Calibration curve of the gallic acid: Y = 14286x + 1395.8 (r = 0.9996); catechin: Y = 15097x + 1189.3 (r = 0.9997); caffeic acid: Y = 12758x + 1259.7 (r = 0.9996); chlorogenic acid: Y = 13461x + 1275.3 (r = 0.9992); ellagic acid: Y = 13576x + 1346.4 (r = 0.9999); rutin: Y = 12845 + 1305.7 (r = 0.9999); quercetin: Y = 13560x + 1192.6 (r = 0.9991); isoquercitrin: Y = 12873x + 1325.6 (r = 0.9998); quercitrin: Y = 11870x + 1329.8 (r = 0.9993); and kaempferol: Y = 14253x + 1238.9 (r = 0.9997).
Mentions: The main compounds present in the Mt-OH fraction were caffeic acid (32.47 ± 0.03 mg/g) and quercitrin (31.96 ± 0.03 mg/g) while catechin (2.69 ± 0.01 mg/g) and the gallic acid (5.47 ± 0.03 mg/g) were the least abundant. In the Ac-OEt fraction, major compounds were quercitrin (32.97 ± 0.037 mg/g) and isoquercitrin (31.56 ± 0.01 mg/g) while catechin (3.16 ± 0.02 mg/g) and rutin (5.49 ± 0.02 mg/g) were the least present (Figure 1 and Table 1). The chromatographic profile of HEDF demonstrated the presence of caffeic acid and rutin as major compounds (33.17 ± 0.03 mg/g and 20.05 ± 0.01 mg/g, resp.) while gallic acid (5.29 ± 0.01 mg/g) and catechin (5.31 ± 0.01 mg/g) were the least abundant [6].

Bottom Line: Conclusion.The highest levels of phenols and total flavonoids were marked with antioxidant effect.This is the first report of bioactivity of the synergic effect of HEDF and fractions.

View Article: PubMed Central - PubMed

Affiliation: Department of Biological Chemistry, Laboratory of Pharmacology and Molecular Chemistry (LFQM), Regional University of Cariri (URCA), 63100-000 Crato, CE, Brazil; Department of Biochemistry and Molecular Biology, Postgraduate Program in Toxicological Biochemistry, Federal University of Santa Maria (UFSM), 97105-900 Santa Maria, RS, Brazil.

ABSTRACT

Unlabelled: Background. Duguetia furfuracea is popular plant used in popular medicine. Hypothesis/Purpose. This claim evaluated the phytochemical composition of the hydroethanolic extract (HEDF), fractions of Duguetia furfuracea, and antioxidant and antifungal activity. Methods. The chemical profile was carried out by HPLC-DAD. The total phenolic contents and flavonoid components were determined by Folin-Ciocalteu and aluminium chloride reaction. The antioxidant activity was measured by scavenging of 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical and ferric reducing ability of plasma (FRAP) methods. The antifungal activity was determined by microdilution assay.

Results: HPLC analysis revealed caffeic acid and rutin as major compounds (HEDF), caffeic acid and quercitrin (Mt-OH fraction), and quercitrin and isoquercitrin (Ac-OEt fraction). The highest levels of phenols and total flavonoids were found for Ac-OEt fraction, and the crude extract showed higher in vitro antioxidant potential. The antifungal activity showed synergic effect with fluconazole and EHDF against C. krusei, fluconazole and Mt-OH against C. krusei and C. tropicalis, and Ac-OE and fluconazole against C. albicans. Conclusion. The highest levels of phenols and total flavonoids were marked with antioxidant effect. This is the first report of bioactivity of the synergic effect of HEDF and fractions. More studies would be required to better clarify its mechanism of synergic action.

No MeSH data available.


Related in: MedlinePlus