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Bubaline Cholecyst Derived Extracellular Matrix for Reconstruction of Full Thickness Skin Wounds in Rats.

Shakya P, Sharma AK, Kumar N, Vellachi R, Mathew DD, Dubey P, Singh K, Shrivastava S, Shrivastava S, Maiti SK, Hasan A, Singh KP - Scientifica (Cairo) (2016)

Bottom Line: In group II, the defect was repaired with commercially available collagen sheet (b-CS).In group III, the defect was repaired with cholecyst derived extracellular matrix of bovine origin (b-CEM).Histologically, improved epithelization, neovascularization, fibroplasia, and best arranged collagen fibers were observed in b-CEM (III) as early as on postimplantation day 21.

View Article: PubMed Central - PubMed

Affiliation: Division of Surgery, Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122, India.

ABSTRACT
An acellular cholecyst derived extracellular matrix (b-CEM) of bubaline origin was prepared using anionic biological detergent. Healing potential of b-CEM was compared with commercially available collagen sheet (b-CS) and open wound (C) in full thickness skin wounds in rats. Thirty-six clinically healthy adult Sprague Dawley rats of either sex were randomly divided into three equal groups. Under general anesthesia, a full thickness skin wound (20 × 20 mm(2)) was created on the dorsum of each rat. The defect in group I was kept as open wound and was taken as control. In group II, the defect was repaired with commercially available collagen sheet (b-CS). In group III, the defect was repaired with cholecyst derived extracellular matrix of bovine origin (b-CEM). Planimetry, wound contracture, and immunological and histological observations were carried out to evaluate healing process. Significantly (P < 0.05) increased wound contraction was observed in b-CEM (III) as compared to control (I) and b-CS (II) on day 21. Histologically, improved epithelization, neovascularization, fibroplasia, and best arranged collagen fibers were observed in b-CEM (III) as early as on postimplantation day 21. These findings indicate that b-CEM have potential for biomedical applications for full thickness skin wound repair in rats.

No MeSH data available.


Related in: MedlinePlus

Digital colour photographs of different groups at different time intervals.
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Related In: Results  -  Collection


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fig5: Digital colour photographs of different groups at different time intervals.

Mentions: During the entire period of the study none of the wounds showed any visible suppurative inflammation. Furthermore, none of the animals became sick or died. The representative images of wound of one animal in each group, at baseline (day zero) and end of healing, are presented in Figure 5. On day 3, in control (I), wounds were covered with soft and fragile pinkish mass above the wound surface area with serous exudates oozing out. On day 7, the surface became more desiccated and necrosed with some amount of exudates. By day 14, the wound size decreased markedly and a thick crust developed and it starts detaching leaving a raw granular pink tissue. By day 28, wound healed up completely by severe contraction leaving a large scar. In b-CS(II), on day 3, the top layer of commercially collagen sheet implanted wound covered with soft and fragile pinkish mass necrosed margin with exudates. By day 7, the top layer of graft got dried and turned up brown. By day 14, the upper layer became more desiccated and shriveled. On day 28, the wound was not completely healed. In b-CEM on day 3, the top layer of acellular grafts appeared dark brown in colour. By day 7, it got dried and turned up brown. On day 14, it was seen in a stage of detachment from the underlying tissue as only the ends sutured holding it in place. On day 21, the dried-up top layer was completely sloughed off and newly formed granulation tissue within the under lying acellular graft covered the entire surface of the wound. By day 28, size of the wound decreased markedly. The wound edges healed completely and the remaining granulation tissue in the centre dried up indicating complete healing beneath it.


Bubaline Cholecyst Derived Extracellular Matrix for Reconstruction of Full Thickness Skin Wounds in Rats.

Shakya P, Sharma AK, Kumar N, Vellachi R, Mathew DD, Dubey P, Singh K, Shrivastava S, Shrivastava S, Maiti SK, Hasan A, Singh KP - Scientifica (Cairo) (2016)

Digital colour photographs of different groups at different time intervals.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4835655&req=5

fig5: Digital colour photographs of different groups at different time intervals.
Mentions: During the entire period of the study none of the wounds showed any visible suppurative inflammation. Furthermore, none of the animals became sick or died. The representative images of wound of one animal in each group, at baseline (day zero) and end of healing, are presented in Figure 5. On day 3, in control (I), wounds were covered with soft and fragile pinkish mass above the wound surface area with serous exudates oozing out. On day 7, the surface became more desiccated and necrosed with some amount of exudates. By day 14, the wound size decreased markedly and a thick crust developed and it starts detaching leaving a raw granular pink tissue. By day 28, wound healed up completely by severe contraction leaving a large scar. In b-CS(II), on day 3, the top layer of commercially collagen sheet implanted wound covered with soft and fragile pinkish mass necrosed margin with exudates. By day 7, the top layer of graft got dried and turned up brown. By day 14, the upper layer became more desiccated and shriveled. On day 28, the wound was not completely healed. In b-CEM on day 3, the top layer of acellular grafts appeared dark brown in colour. By day 7, it got dried and turned up brown. On day 14, it was seen in a stage of detachment from the underlying tissue as only the ends sutured holding it in place. On day 21, the dried-up top layer was completely sloughed off and newly formed granulation tissue within the under lying acellular graft covered the entire surface of the wound. By day 28, size of the wound decreased markedly. The wound edges healed completely and the remaining granulation tissue in the centre dried up indicating complete healing beneath it.

Bottom Line: In group II, the defect was repaired with commercially available collagen sheet (b-CS).In group III, the defect was repaired with cholecyst derived extracellular matrix of bovine origin (b-CEM).Histologically, improved epithelization, neovascularization, fibroplasia, and best arranged collagen fibers were observed in b-CEM (III) as early as on postimplantation day 21.

View Article: PubMed Central - PubMed

Affiliation: Division of Surgery, Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122, India.

ABSTRACT
An acellular cholecyst derived extracellular matrix (b-CEM) of bubaline origin was prepared using anionic biological detergent. Healing potential of b-CEM was compared with commercially available collagen sheet (b-CS) and open wound (C) in full thickness skin wounds in rats. Thirty-six clinically healthy adult Sprague Dawley rats of either sex were randomly divided into three equal groups. Under general anesthesia, a full thickness skin wound (20 × 20 mm(2)) was created on the dorsum of each rat. The defect in group I was kept as open wound and was taken as control. In group II, the defect was repaired with commercially available collagen sheet (b-CS). In group III, the defect was repaired with cholecyst derived extracellular matrix of bovine origin (b-CEM). Planimetry, wound contracture, and immunological and histological observations were carried out to evaluate healing process. Significantly (P < 0.05) increased wound contraction was observed in b-CEM (III) as compared to control (I) and b-CS (II) on day 21. Histologically, improved epithelization, neovascularization, fibroplasia, and best arranged collagen fibers were observed in b-CEM (III) as early as on postimplantation day 21. These findings indicate that b-CEM have potential for biomedical applications for full thickness skin wound repair in rats.

No MeSH data available.


Related in: MedlinePlus