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Bubaline Cholecyst Derived Extracellular Matrix for Reconstruction of Full Thickness Skin Wounds in Rats.

Shakya P, Sharma AK, Kumar N, Vellachi R, Mathew DD, Dubey P, Singh K, Shrivastava S, Shrivastava S, Maiti SK, Hasan A, Singh KP - Scientifica (Cairo) (2016)

Bottom Line: In group II, the defect was repaired with commercially available collagen sheet (b-CS).In group III, the defect was repaired with cholecyst derived extracellular matrix of bovine origin (b-CEM).Histologically, improved epithelization, neovascularization, fibroplasia, and best arranged collagen fibers were observed in b-CEM (III) as early as on postimplantation day 21.

View Article: PubMed Central - PubMed

Affiliation: Division of Surgery, Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122, India.

ABSTRACT
An acellular cholecyst derived extracellular matrix (b-CEM) of bubaline origin was prepared using anionic biological detergent. Healing potential of b-CEM was compared with commercially available collagen sheet (b-CS) and open wound (C) in full thickness skin wounds in rats. Thirty-six clinically healthy adult Sprague Dawley rats of either sex were randomly divided into three equal groups. Under general anesthesia, a full thickness skin wound (20 × 20 mm(2)) was created on the dorsum of each rat. The defect in group I was kept as open wound and was taken as control. In group II, the defect was repaired with commercially available collagen sheet (b-CS). In group III, the defect was repaired with cholecyst derived extracellular matrix of bovine origin (b-CEM). Planimetry, wound contracture, and immunological and histological observations were carried out to evaluate healing process. Significantly (P < 0.05) increased wound contraction was observed in b-CEM (III) as compared to control (I) and b-CS (II) on day 21. Histologically, improved epithelization, neovascularization, fibroplasia, and best arranged collagen fibers were observed in b-CEM (III) as early as on postimplantation day 21. These findings indicate that b-CEM have potential for biomedical applications for full thickness skin wound repair in rats.

No MeSH data available.


Related in: MedlinePlus

Microphotograph of native bovine gallbladder after delamination showing cellularity, loose muscular layer, and collagen fibers (H&E stain, 200x).
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Related In: Results  -  Collection


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fig1: Microphotograph of native bovine gallbladder after delamination showing cellularity, loose muscular layer, and collagen fibers (H&E stain, 200x).

Mentions: Microscopically, native bovine gallbladder after delamination showed cellularity, loose muscular layer, and collagen fibers (Figure 1). Masson's trichrome staining also showed dense compact arrangement of collagen fibers (Figure 2). The delaminated gallbladder treated with 0.5% ionic biological detergent for 12 h under constant agitation showed complete loss of cellularity. The submucosal layers were completely acellular (Figure 3). The collagen fibers were compact with moderate porosity than the native tissue (Figure 4).


Bubaline Cholecyst Derived Extracellular Matrix for Reconstruction of Full Thickness Skin Wounds in Rats.

Shakya P, Sharma AK, Kumar N, Vellachi R, Mathew DD, Dubey P, Singh K, Shrivastava S, Shrivastava S, Maiti SK, Hasan A, Singh KP - Scientifica (Cairo) (2016)

Microphotograph of native bovine gallbladder after delamination showing cellularity, loose muscular layer, and collagen fibers (H&E stain, 200x).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4835655&req=5

fig1: Microphotograph of native bovine gallbladder after delamination showing cellularity, loose muscular layer, and collagen fibers (H&E stain, 200x).
Mentions: Microscopically, native bovine gallbladder after delamination showed cellularity, loose muscular layer, and collagen fibers (Figure 1). Masson's trichrome staining also showed dense compact arrangement of collagen fibers (Figure 2). The delaminated gallbladder treated with 0.5% ionic biological detergent for 12 h under constant agitation showed complete loss of cellularity. The submucosal layers were completely acellular (Figure 3). The collagen fibers were compact with moderate porosity than the native tissue (Figure 4).

Bottom Line: In group II, the defect was repaired with commercially available collagen sheet (b-CS).In group III, the defect was repaired with cholecyst derived extracellular matrix of bovine origin (b-CEM).Histologically, improved epithelization, neovascularization, fibroplasia, and best arranged collagen fibers were observed in b-CEM (III) as early as on postimplantation day 21.

View Article: PubMed Central - PubMed

Affiliation: Division of Surgery, Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122, India.

ABSTRACT
An acellular cholecyst derived extracellular matrix (b-CEM) of bubaline origin was prepared using anionic biological detergent. Healing potential of b-CEM was compared with commercially available collagen sheet (b-CS) and open wound (C) in full thickness skin wounds in rats. Thirty-six clinically healthy adult Sprague Dawley rats of either sex were randomly divided into three equal groups. Under general anesthesia, a full thickness skin wound (20 × 20 mm(2)) was created on the dorsum of each rat. The defect in group I was kept as open wound and was taken as control. In group II, the defect was repaired with commercially available collagen sheet (b-CS). In group III, the defect was repaired with cholecyst derived extracellular matrix of bovine origin (b-CEM). Planimetry, wound contracture, and immunological and histological observations were carried out to evaluate healing process. Significantly (P < 0.05) increased wound contraction was observed in b-CEM (III) as compared to control (I) and b-CS (II) on day 21. Histologically, improved epithelization, neovascularization, fibroplasia, and best arranged collagen fibers were observed in b-CEM (III) as early as on postimplantation day 21. These findings indicate that b-CEM have potential for biomedical applications for full thickness skin wound repair in rats.

No MeSH data available.


Related in: MedlinePlus