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The Efficacy of Umbelliferone, Arbutin, and N-Acetylcysteine to Prevent Microbial Colonization and Biofilm Development on Urinary Catheter Surface: Results from a Preliminary Study.

Cai T, Gallelli L, Meacci F, Brugnolli A, Prosperi L, Roberta S, Eccher C, Mazzoli S, Lanzafame P, Caciagli P, Malossini G, Bartoletti R - J Pathog (2016)

Bottom Line: A significantly lower amount in planktonic (p < 0.001) and sessile (p = 0.004) bacterial load was found in group 3, showing <100 CFU/mL and 0.12 × 10(6) CFU/cm in the incubation medium and on the catheter surface, respectively.In groups 1 and 2, 1.67 × 10(6) CFU/cm and 1.77 × 10(6) CFU/cm were found on catheter surface.Our results document that umbelliferone, arbutin, and N-acetylcysteine are able to reduce E. faecalis biofilm development on the surface of urinary catheters.

View Article: PubMed Central - PubMed

Affiliation: Department of Urology, Santa Chiara Regional Hospital, 38123 Trento, Italy.

ABSTRACT
We evaluated, in a preliminary study, the efficacy of umbelliferone, arbutin, and N-acetylcysteine to inhibit biofilm formation on urinary catheter. We used 20 urinary catheters: 5 catheters were incubated with Enterococcus faecalis (control group); 5 catheters were incubated with E. faecalis in presence of umbelliferone (150 mg), arbutin (60 mg), and N-acetylcysteine (150 mg) (group 1); 5 catheters were incubated with E. faecalis in presence of umbelliferone (150 mg), arbutin (60 mg), and N-acetylcysteine (400 mg) (group 2); and 5 catheters were incubated with E. faecalis in presence of umbelliferone (300 mg), arbutin (60 mg), and N-acetylcysteine (150 mg) (group 3). After 72 hours, planktonic microbial growth and microorganisms on catheter surface were assessed. In the control group, we found a planktonic load of ≥10(5) CFU/mL in the inoculation medium and retrieved 3.69 × 10(6) CFU/cm from the sessile cells adherent to the catheter surface. A significantly lower amount in planktonic (p < 0.001) and sessile (p = 0.004) bacterial load was found in group 3, showing <100 CFU/mL and 0.12 × 10(6) CFU/cm in the incubation medium and on the catheter surface, respectively. In groups 1 and 2, 1.67 × 10(6) CFU/cm and 1.77 × 10(6) CFU/cm were found on catheter surface. Our results document that umbelliferone, arbutin, and N-acetylcysteine are able to reduce E. faecalis biofilm development on the surface of urinary catheters.

No MeSH data available.


The urinary catheter preparation procedure for the experiment.
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fig1: The urinary catheter preparation procedure for the experiment.

Mentions: Twenty-five urinary catheters (Silkolatex® Rüsch Gold® Size Ch. 22) were used. Five catheters were used for the training phase and 20 for the experimental study. The proximal 5 cm long segment of each catheter was aseptically separated with a sterile scalpel (Figure 1). Separated bioreactors were set up for each catheter segment during the experimental phase according to the following procedure. The catheter segment was put in a Falcon tube (Falcon® Brand Products; Life Sciences Brands) and 40 mL of E. faecalis suspension (5 × 105  colony-forming units (CFU)/mL) in Mueller-Hinton broth (Sigma-Aldrich Co., LLC., USA) was added. Then, 5 mL of different solutions was poured in each bioreactor, according to the following experimental groups: 


The Efficacy of Umbelliferone, Arbutin, and N-Acetylcysteine to Prevent Microbial Colonization and Biofilm Development on Urinary Catheter Surface: Results from a Preliminary Study.

Cai T, Gallelli L, Meacci F, Brugnolli A, Prosperi L, Roberta S, Eccher C, Mazzoli S, Lanzafame P, Caciagli P, Malossini G, Bartoletti R - J Pathog (2016)

The urinary catheter preparation procedure for the experiment.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4835653&req=5

fig1: The urinary catheter preparation procedure for the experiment.
Mentions: Twenty-five urinary catheters (Silkolatex® Rüsch Gold® Size Ch. 22) were used. Five catheters were used for the training phase and 20 for the experimental study. The proximal 5 cm long segment of each catheter was aseptically separated with a sterile scalpel (Figure 1). Separated bioreactors were set up for each catheter segment during the experimental phase according to the following procedure. The catheter segment was put in a Falcon tube (Falcon® Brand Products; Life Sciences Brands) and 40 mL of E. faecalis suspension (5 × 105  colony-forming units (CFU)/mL) in Mueller-Hinton broth (Sigma-Aldrich Co., LLC., USA) was added. Then, 5 mL of different solutions was poured in each bioreactor, according to the following experimental groups: 

Bottom Line: A significantly lower amount in planktonic (p < 0.001) and sessile (p = 0.004) bacterial load was found in group 3, showing <100 CFU/mL and 0.12 × 10(6) CFU/cm in the incubation medium and on the catheter surface, respectively.In groups 1 and 2, 1.67 × 10(6) CFU/cm and 1.77 × 10(6) CFU/cm were found on catheter surface.Our results document that umbelliferone, arbutin, and N-acetylcysteine are able to reduce E. faecalis biofilm development on the surface of urinary catheters.

View Article: PubMed Central - PubMed

Affiliation: Department of Urology, Santa Chiara Regional Hospital, 38123 Trento, Italy.

ABSTRACT
We evaluated, in a preliminary study, the efficacy of umbelliferone, arbutin, and N-acetylcysteine to inhibit biofilm formation on urinary catheter. We used 20 urinary catheters: 5 catheters were incubated with Enterococcus faecalis (control group); 5 catheters were incubated with E. faecalis in presence of umbelliferone (150 mg), arbutin (60 mg), and N-acetylcysteine (150 mg) (group 1); 5 catheters were incubated with E. faecalis in presence of umbelliferone (150 mg), arbutin (60 mg), and N-acetylcysteine (400 mg) (group 2); and 5 catheters were incubated with E. faecalis in presence of umbelliferone (300 mg), arbutin (60 mg), and N-acetylcysteine (150 mg) (group 3). After 72 hours, planktonic microbial growth and microorganisms on catheter surface were assessed. In the control group, we found a planktonic load of ≥10(5) CFU/mL in the inoculation medium and retrieved 3.69 × 10(6) CFU/cm from the sessile cells adherent to the catheter surface. A significantly lower amount in planktonic (p < 0.001) and sessile (p = 0.004) bacterial load was found in group 3, showing <100 CFU/mL and 0.12 × 10(6) CFU/cm in the incubation medium and on the catheter surface, respectively. In groups 1 and 2, 1.67 × 10(6) CFU/cm and 1.77 × 10(6) CFU/cm were found on catheter surface. Our results document that umbelliferone, arbutin, and N-acetylcysteine are able to reduce E. faecalis biofilm development on the surface of urinary catheters.

No MeSH data available.