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Data in support of intermolecular interactions at early stage of protein/detergent particle association induced by salt/polyethylene glycol mixtures.

Odahara T, Odahara K - Data Brief (2016)

Bottom Line: The data provide information in support of the research article, "Intermolecular interactions at early stage of protein/detergent particle association induced by salt/polyethylene glycol mixtures" [1].The data regarding variation of absorption spectra is used as an indicator of the duration of Rp. viridis PRU and RC, Rb. sphaeroides RC and LH2, and Rb. capsulatus LH2 in the native state in the presence of NaCl/polyethylene glycol (PEG) mixture.The data about minimum concentrations of salt and PEG whose aqueous phases are mutually separated presents information on additional influence of Tris buffer and N-octyl-β-d-glucoside on the salt-PEG phase separation.

View Article: PubMed Central - PubMed

Affiliation: National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Central-6, 1-1 Higashi, Tsukuba, Ibaraki 305-8566, Japan.

ABSTRACT
The data provide information in support of the research article, "Intermolecular interactions at early stage of protein/detergent particle association induced by salt/polyethylene glycol mixtures" [1]. The data regarding variation of absorption spectra is used as an indicator of the duration of Rp. viridis PRU and RC, Rb. sphaeroides RC and LH2, and Rb. capsulatus LH2 in the native state in the presence of NaCl/polyethylene glycol (PEG) mixture. The data about minimum concentrations of salt and PEG whose aqueous phases are mutually separated presents information on additional influence of Tris buffer and N-octyl-β-d-glucoside on the salt-PEG phase separation.

No MeSH data available.


Related in: MedlinePlus

Minimum PEG concentrations at various salt concentrations for the formation of immiscible salt- and PEG-aqueous phases in the presence of 25 mM Tris buffer and 8 mg/mL OG at room temperature (21–24 °C). The salts examined were di-potassium tartrate, di–potassium hydrogen phosphate, and tri–potassium citrate. The solution ingredients were potassium tartrate in water (○), 25 mM Tris–HCl (□) and 25 mM Tris–HCl and 8 mg/mL OG (◇), K2HPO4 in water (•), 25 mM Tris–HCl (■) and 25 mM Tris–HCl and 8 mg/mL OG (♦), and potassium citrate in water (✕), 25 mM Tris–HCl (+) and 25 mM Tris–HCl and 8 mg/mL OG (Δ).
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f0010: Minimum PEG concentrations at various salt concentrations for the formation of immiscible salt- and PEG-aqueous phases in the presence of 25 mM Tris buffer and 8 mg/mL OG at room temperature (21–24 °C). The salts examined were di-potassium tartrate, di–potassium hydrogen phosphate, and tri–potassium citrate. The solution ingredients were potassium tartrate in water (○), 25 mM Tris–HCl (□) and 25 mM Tris–HCl and 8 mg/mL OG (◇), K2HPO4 in water (•), 25 mM Tris–HCl (■) and 25 mM Tris–HCl and 8 mg/mL OG (♦), and potassium citrate in water (✕), 25 mM Tris–HCl (+) and 25 mM Tris–HCl and 8 mg/mL OG (Δ).

Mentions: Minimum PEG concentrations for phase separation at various salt concentrations were determined in the presence of 25 mM Tris buffer and 8 mg/mL OG, as follows. A concentrated salt solution, a 625 mg/mL PEG solution, a 400 mM Tris–HCl solution (pH 8.0), a 200 mg/mL OG solution and pure water were put in small glass tubes at different ratios, and the mixture was shaken vigorously on a vortex mixer. Formation of mutually immiscible phases was judged by observing with eyes whether the resultant mixtures were turbid. The result is shown in Fig. 2. Two symbols at each salt concentration in the figure represent the highest PEG concentration where clear (single-phase) solutions were formed and the lowest PEG concentration where turbid solutions (containing two immiscible phases) were formed. The true minimum PEG concentrations for the phase separation should be of values between the two PEG concentrations. With each of the three salts, the minimum PEG concentration [P] varied linearly against the logarithm of the salt concentration [S]. Hence, the [P]–[S] line for each salt was calculated by least-square fitting of the relationship, [P]=Aps−Bps log [S], to the two PEG concentrations at various salt concentrations, where Aps and Bps were constants. The addition of Tris–HCl and OG exhibited an effect to lower the minimum PEG concentrations by 20 mg/mL at maximum. This effect was approximately similar to or less than the effect of the three salts at 25 mN.


Data in support of intermolecular interactions at early stage of protein/detergent particle association induced by salt/polyethylene glycol mixtures.

Odahara T, Odahara K - Data Brief (2016)

Minimum PEG concentrations at various salt concentrations for the formation of immiscible salt- and PEG-aqueous phases in the presence of 25 mM Tris buffer and 8 mg/mL OG at room temperature (21–24 °C). The salts examined were di-potassium tartrate, di–potassium hydrogen phosphate, and tri–potassium citrate. The solution ingredients were potassium tartrate in water (○), 25 mM Tris–HCl (□) and 25 mM Tris–HCl and 8 mg/mL OG (◇), K2HPO4 in water (•), 25 mM Tris–HCl (■) and 25 mM Tris–HCl and 8 mg/mL OG (♦), and potassium citrate in water (✕), 25 mM Tris–HCl (+) and 25 mM Tris–HCl and 8 mg/mL OG (Δ).
© Copyright Policy - CC BY
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4835637&req=5

f0010: Minimum PEG concentrations at various salt concentrations for the formation of immiscible salt- and PEG-aqueous phases in the presence of 25 mM Tris buffer and 8 mg/mL OG at room temperature (21–24 °C). The salts examined were di-potassium tartrate, di–potassium hydrogen phosphate, and tri–potassium citrate. The solution ingredients were potassium tartrate in water (○), 25 mM Tris–HCl (□) and 25 mM Tris–HCl and 8 mg/mL OG (◇), K2HPO4 in water (•), 25 mM Tris–HCl (■) and 25 mM Tris–HCl and 8 mg/mL OG (♦), and potassium citrate in water (✕), 25 mM Tris–HCl (+) and 25 mM Tris–HCl and 8 mg/mL OG (Δ).
Mentions: Minimum PEG concentrations for phase separation at various salt concentrations were determined in the presence of 25 mM Tris buffer and 8 mg/mL OG, as follows. A concentrated salt solution, a 625 mg/mL PEG solution, a 400 mM Tris–HCl solution (pH 8.0), a 200 mg/mL OG solution and pure water were put in small glass tubes at different ratios, and the mixture was shaken vigorously on a vortex mixer. Formation of mutually immiscible phases was judged by observing with eyes whether the resultant mixtures were turbid. The result is shown in Fig. 2. Two symbols at each salt concentration in the figure represent the highest PEG concentration where clear (single-phase) solutions were formed and the lowest PEG concentration where turbid solutions (containing two immiscible phases) were formed. The true minimum PEG concentrations for the phase separation should be of values between the two PEG concentrations. With each of the three salts, the minimum PEG concentration [P] varied linearly against the logarithm of the salt concentration [S]. Hence, the [P]–[S] line for each salt was calculated by least-square fitting of the relationship, [P]=Aps−Bps log [S], to the two PEG concentrations at various salt concentrations, where Aps and Bps were constants. The addition of Tris–HCl and OG exhibited an effect to lower the minimum PEG concentrations by 20 mg/mL at maximum. This effect was approximately similar to or less than the effect of the three salts at 25 mN.

Bottom Line: The data provide information in support of the research article, "Intermolecular interactions at early stage of protein/detergent particle association induced by salt/polyethylene glycol mixtures" [1].The data regarding variation of absorption spectra is used as an indicator of the duration of Rp. viridis PRU and RC, Rb. sphaeroides RC and LH2, and Rb. capsulatus LH2 in the native state in the presence of NaCl/polyethylene glycol (PEG) mixture.The data about minimum concentrations of salt and PEG whose aqueous phases are mutually separated presents information on additional influence of Tris buffer and N-octyl-β-d-glucoside on the salt-PEG phase separation.

View Article: PubMed Central - PubMed

Affiliation: National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Central-6, 1-1 Higashi, Tsukuba, Ibaraki 305-8566, Japan.

ABSTRACT
The data provide information in support of the research article, "Intermolecular interactions at early stage of protein/detergent particle association induced by salt/polyethylene glycol mixtures" [1]. The data regarding variation of absorption spectra is used as an indicator of the duration of Rp. viridis PRU and RC, Rb. sphaeroides RC and LH2, and Rb. capsulatus LH2 in the native state in the presence of NaCl/polyethylene glycol (PEG) mixture. The data about minimum concentrations of salt and PEG whose aqueous phases are mutually separated presents information on additional influence of Tris buffer and N-octyl-β-d-glucoside on the salt-PEG phase separation.

No MeSH data available.


Related in: MedlinePlus