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Curcumin Downregulates Phosphate Carrier and Protects against Doxorubicin Induced Cardiomyocyte Apoptosis.

Junkun L, Erfu C, Tony H, Xin L, Sudeep KC, Mingliang Z, Yanqin W, XiangQian Q - Biomed Res Int (2016)

Bottom Line: Results.Interestingly, the effect was not clearly dose dependent and the concentration of 12 mg/L was more efficient than 15 and 10 mg/L.Conclusion.

View Article: PubMed Central - PubMed

Affiliation: Department of Cardiology Division II, Tianjin Medical University Cardiovascular Institute, TEDA International Cardiovascular Hospital, Tianjin 300070, China; Department of Cardiology Division II, Jiamusi University, 1st Affiliated Hospital, Jiamusi, Heilongjiang 154003, China.

ABSTRACT
Aim. To explore the effects of curcumin on phosphate carrier (PiC) and its role in protection against doxorubicin induced myocyte toxicity. Methods. Using H9c2 cell line, the cardiotoxic effect of doxorubicin and its mitigation by curcumin were studied. H9c2 cells were cultured with doxorubicin and/or curcumin at various concentrations. Analysis for apoptosis of H9c2 was done using flow cytometry. Confocal laser scanning microscopy was used to record the fluorescence intensity ratios and to determine the mitochondrial permeability transition pore (MPTP) opening state. Oxidative stress was measured using glutathione level, superoxide dismutase activities, and malondialdehyde content. The effect of doxorubicin and curcumin on PiC gene expression was measured by real-time PCR. Results. Curcumin decreased mRNA of PiC and was partly protective against oxidative stress, loss of mitochondrial transmembrane potential, and apoptosis induced by doxorubicin. Interestingly, the effect was not clearly dose dependent and the concentration of 12 mg/L was more efficient than 15 and 10 mg/L. Conclusion. Curcumin downregulates PiC and partly protects against doxorubicin induced oxidative stress and myocyte apoptosis.

No MeSH data available.


Related in: MedlinePlus

The expression of PiC (Slc25a3) gene was significantly increased by doxorubicin, while curcumin decreased PiC expression. In addition, the PiC in groups of Cur 0 was also significantly increased over time following doxorubicin treatment of the cells. (∗P < 0.05 versus Cur 0, Dox + at each time point) (#P < 0.05 versus negative control (Dox 0 h, Cur 0 min)).
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fig2: The expression of PiC (Slc25a3) gene was significantly increased by doxorubicin, while curcumin decreased PiC expression. In addition, the PiC in groups of Cur 0 was also significantly increased over time following doxorubicin treatment of the cells. (∗P < 0.05 versus Cur 0, Dox + at each time point) (#P < 0.05 versus negative control (Dox 0 h, Cur 0 min)).

Mentions: We investigated the effect of doxorubicin and curcumin on PiC gene expression. We found that phosphate carrier gene expression in cardiomyocytes was significantly decreased by curcumin and increased by doxorubicin (Figure 2). At zero hours, we noted an initial increase in PiC following addition of curcumin. This could be due to the fact that curcumin itself, even in lower concentrations, also has some level of toxicity [11], to which the cells may adapt over time. 12 mg/L curcumin was the most effective dose in decreasing PiC.


Curcumin Downregulates Phosphate Carrier and Protects against Doxorubicin Induced Cardiomyocyte Apoptosis.

Junkun L, Erfu C, Tony H, Xin L, Sudeep KC, Mingliang Z, Yanqin W, XiangQian Q - Biomed Res Int (2016)

The expression of PiC (Slc25a3) gene was significantly increased by doxorubicin, while curcumin decreased PiC expression. In addition, the PiC in groups of Cur 0 was also significantly increased over time following doxorubicin treatment of the cells. (∗P < 0.05 versus Cur 0, Dox + at each time point) (#P < 0.05 versus negative control (Dox 0 h, Cur 0 min)).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4835619&req=5

fig2: The expression of PiC (Slc25a3) gene was significantly increased by doxorubicin, while curcumin decreased PiC expression. In addition, the PiC in groups of Cur 0 was also significantly increased over time following doxorubicin treatment of the cells. (∗P < 0.05 versus Cur 0, Dox + at each time point) (#P < 0.05 versus negative control (Dox 0 h, Cur 0 min)).
Mentions: We investigated the effect of doxorubicin and curcumin on PiC gene expression. We found that phosphate carrier gene expression in cardiomyocytes was significantly decreased by curcumin and increased by doxorubicin (Figure 2). At zero hours, we noted an initial increase in PiC following addition of curcumin. This could be due to the fact that curcumin itself, even in lower concentrations, also has some level of toxicity [11], to which the cells may adapt over time. 12 mg/L curcumin was the most effective dose in decreasing PiC.

Bottom Line: Results.Interestingly, the effect was not clearly dose dependent and the concentration of 12 mg/L was more efficient than 15 and 10 mg/L.Conclusion.

View Article: PubMed Central - PubMed

Affiliation: Department of Cardiology Division II, Tianjin Medical University Cardiovascular Institute, TEDA International Cardiovascular Hospital, Tianjin 300070, China; Department of Cardiology Division II, Jiamusi University, 1st Affiliated Hospital, Jiamusi, Heilongjiang 154003, China.

ABSTRACT
Aim. To explore the effects of curcumin on phosphate carrier (PiC) and its role in protection against doxorubicin induced myocyte toxicity. Methods. Using H9c2 cell line, the cardiotoxic effect of doxorubicin and its mitigation by curcumin were studied. H9c2 cells were cultured with doxorubicin and/or curcumin at various concentrations. Analysis for apoptosis of H9c2 was done using flow cytometry. Confocal laser scanning microscopy was used to record the fluorescence intensity ratios and to determine the mitochondrial permeability transition pore (MPTP) opening state. Oxidative stress was measured using glutathione level, superoxide dismutase activities, and malondialdehyde content. The effect of doxorubicin and curcumin on PiC gene expression was measured by real-time PCR. Results. Curcumin decreased mRNA of PiC and was partly protective against oxidative stress, loss of mitochondrial transmembrane potential, and apoptosis induced by doxorubicin. Interestingly, the effect was not clearly dose dependent and the concentration of 12 mg/L was more efficient than 15 and 10 mg/L. Conclusion. Curcumin downregulates PiC and partly protects against doxorubicin induced oxidative stress and myocyte apoptosis.

No MeSH data available.


Related in: MedlinePlus