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Estrogen Modulates Expression of Tight Junction Proteins in Rat Vagina.

Oh KJ, Lee HS, Ahn K, Park K - Biomed Res Int (2016)

Bottom Line: Results.Tight junctions are distinctly localized in rat vagina, and estrogen modulates the expression of tight junctions.Further researches are needed to clarify the functional role of tight junctions in vaginal lubrication.

View Article: PubMed Central - PubMed

Affiliation: Department of Urology, Chonnam National University Medical School, Sexual Medicine Research Center, Chonnam National University, Gwangju 61469, Republic of Korea.

ABSTRACT
Background. The objectives of this study were to investigate the localization of tight junctions and the modulation of zonula occludens- (ZO-) 1, occludin and claudin-1 expression by estrogen in castrated female rat vagina. Female Sprague-Dawley rats (230-240 g, n = 45) were divided into three groups and subjected to a sham operation (control group, n = 15), bilateral ovariectomy (Ovx group, n = 15), or bilateral ovariectomy followed by daily subcutaneous injection of 17β-estradiol (50 μg/kg/day, Ovx + Est group, n = 15). The cellular localization and expression of ZO-1, occludin, and claudin-1 were determined in each group by immunohistochemistry and western blot. Results. Expression of ZO-1 was diffuse in all groups, with the highest intensity in the superficial epithelium in the control group. Occludin was localized in the intermediate and basal epithelium. Claudin-1 was most intense in the superficial layer of the vaginal epithelium in the control group. Expression of ZO-1, occludin, and claudin-1 was significantly decreased after ovariectomy and was restored to the level of the control after estrogen replacement. Conclusions. Tight junctions are distinctly localized in rat vagina, and estrogen modulates the expression of tight junctions. Further researches are needed to clarify the functional role of tight junctions in vaginal lubrication.

No MeSH data available.


Related in: MedlinePlus

(a) Immunohistochemistry of occludin in vaginal tissue from animals of the control (Con), ovariectomy (Ovx), and ovariectomy plus 17β-estradiol treatment (Ovx + Est) groups. Occludin was expressed in the intermediate and basal epithelium. (b) Immunoblotting of occludin in the rat vagina. The lower panels denote the means ± standard error of 6 experiments for each condition determined by densitometry relative to beta-actin. ∗P < 0.05 versus control. ∗∗P < 0.05 versus Ovx.
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fig2: (a) Immunohistochemistry of occludin in vaginal tissue from animals of the control (Con), ovariectomy (Ovx), and ovariectomy plus 17β-estradiol treatment (Ovx + Est) groups. Occludin was expressed in the intermediate and basal epithelium. (b) Immunoblotting of occludin in the rat vagina. The lower panels denote the means ± standard error of 6 experiments for each condition determined by densitometry relative to beta-actin. ∗P < 0.05 versus control. ∗∗P < 0.05 versus Ovx.

Mentions: Vaginal tissue from the control group showed a multiple-layered epithelium. After ovariectomy, the vaginal tissue showed atrophic changes, with a thinner epithelium and mucosal distortion. After treatment with 17β-estradiol, the thickness of the vaginal epithelium was restored and the epithelium looked similar to the control tissue (Figures 1(a), 2(a), and 3(a)).


Estrogen Modulates Expression of Tight Junction Proteins in Rat Vagina.

Oh KJ, Lee HS, Ahn K, Park K - Biomed Res Int (2016)

(a) Immunohistochemistry of occludin in vaginal tissue from animals of the control (Con), ovariectomy (Ovx), and ovariectomy plus 17β-estradiol treatment (Ovx + Est) groups. Occludin was expressed in the intermediate and basal epithelium. (b) Immunoblotting of occludin in the rat vagina. The lower panels denote the means ± standard error of 6 experiments for each condition determined by densitometry relative to beta-actin. ∗P < 0.05 versus control. ∗∗P < 0.05 versus Ovx.
© Copyright Policy
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4835618&req=5

fig2: (a) Immunohistochemistry of occludin in vaginal tissue from animals of the control (Con), ovariectomy (Ovx), and ovariectomy plus 17β-estradiol treatment (Ovx + Est) groups. Occludin was expressed in the intermediate and basal epithelium. (b) Immunoblotting of occludin in the rat vagina. The lower panels denote the means ± standard error of 6 experiments for each condition determined by densitometry relative to beta-actin. ∗P < 0.05 versus control. ∗∗P < 0.05 versus Ovx.
Mentions: Vaginal tissue from the control group showed a multiple-layered epithelium. After ovariectomy, the vaginal tissue showed atrophic changes, with a thinner epithelium and mucosal distortion. After treatment with 17β-estradiol, the thickness of the vaginal epithelium was restored and the epithelium looked similar to the control tissue (Figures 1(a), 2(a), and 3(a)).

Bottom Line: Results.Tight junctions are distinctly localized in rat vagina, and estrogen modulates the expression of tight junctions.Further researches are needed to clarify the functional role of tight junctions in vaginal lubrication.

View Article: PubMed Central - PubMed

Affiliation: Department of Urology, Chonnam National University Medical School, Sexual Medicine Research Center, Chonnam National University, Gwangju 61469, Republic of Korea.

ABSTRACT
Background. The objectives of this study were to investigate the localization of tight junctions and the modulation of zonula occludens- (ZO-) 1, occludin and claudin-1 expression by estrogen in castrated female rat vagina. Female Sprague-Dawley rats (230-240 g, n = 45) were divided into three groups and subjected to a sham operation (control group, n = 15), bilateral ovariectomy (Ovx group, n = 15), or bilateral ovariectomy followed by daily subcutaneous injection of 17β-estradiol (50 μg/kg/day, Ovx + Est group, n = 15). The cellular localization and expression of ZO-1, occludin, and claudin-1 were determined in each group by immunohistochemistry and western blot. Results. Expression of ZO-1 was diffuse in all groups, with the highest intensity in the superficial epithelium in the control group. Occludin was localized in the intermediate and basal epithelium. Claudin-1 was most intense in the superficial layer of the vaginal epithelium in the control group. Expression of ZO-1, occludin, and claudin-1 was significantly decreased after ovariectomy and was restored to the level of the control after estrogen replacement. Conclusions. Tight junctions are distinctly localized in rat vagina, and estrogen modulates the expression of tight junctions. Further researches are needed to clarify the functional role of tight junctions in vaginal lubrication.

No MeSH data available.


Related in: MedlinePlus