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Production of a single cyclic type of fructooligosaccharide structure by inulin-degrading Paenibacillus sp. LX16 newly isolated from Jerusalem artichoke root.

Yao Z, Guo J, Tang W, Sun Z, Hou Y, Li X - Microb Biotechnol (2016)

Bottom Line: The predominant cellular fatty acids were anteiso-C15:0, C16:0 and anteiso-C17:0.The FOS was identified as a cyclofructooligosaccharide with a degree of polymerization of 6.Such homology in inulin degradation products may be beneficial for the functional FOS production.

View Article: PubMed Central - PubMed

Affiliation: School of Biological Engineering, Dalian Polytechnic University, Dalian, 116034, China.

No MeSH data available.


Related in: MedlinePlus

PAGE analysis of the cyclofructooligosaccharide fructanotransferase by silver staining in both native PAGE and SDS/PAGE (A), and the HPLC analysis of the inulin‐degrading products by the purified cyclofructooligosaccharide fructanotransferase (B).
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mbt212358-fig-0005: PAGE analysis of the cyclofructooligosaccharide fructanotransferase by silver staining in both native PAGE and SDS/PAGE (A), and the HPLC analysis of the inulin‐degrading products by the purified cyclofructooligosaccharide fructanotransferase (B).

Mentions: Purification of CFTase was performed from the cell‐free culture of strain LX16 with a specific activity of 2.4 U mg−1, and the results were summarized in Table 3. The preparation gave a total protein recovery of 2.6% in a purification fold of 13.9 and the final product had a specific activity of 33.3 U mg−1. The purified CFTase was analysed by both native PAGE and SDS/PAGE stained with silver staining (Fig. 5A). One single protein band with molecular weight of 101 kDa was observed on the native PAGE, but two bands with molecular weight of 56.2 kDa and 44.8 kDa were observed on the SDS/PAGE. When the enzymatic degradation products of inulin by the purified CFTase were assessed by HPLC (Fig. 5B), one single type of FOS molecule was detected in the process of inulin degradation. The optimum temperature and pH for CFTase activity was 45°C and 7.0 respectively. The CFTase was active at pH ranged from 5.5 to 9.5, and at temperatures up to 55°C.


Production of a single cyclic type of fructooligosaccharide structure by inulin-degrading Paenibacillus sp. LX16 newly isolated from Jerusalem artichoke root.

Yao Z, Guo J, Tang W, Sun Z, Hou Y, Li X - Microb Biotechnol (2016)

PAGE analysis of the cyclofructooligosaccharide fructanotransferase by silver staining in both native PAGE and SDS/PAGE (A), and the HPLC analysis of the inulin‐degrading products by the purified cyclofructooligosaccharide fructanotransferase (B).
© Copyright Policy - creativeCommonsBy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4835578&req=5

mbt212358-fig-0005: PAGE analysis of the cyclofructooligosaccharide fructanotransferase by silver staining in both native PAGE and SDS/PAGE (A), and the HPLC analysis of the inulin‐degrading products by the purified cyclofructooligosaccharide fructanotransferase (B).
Mentions: Purification of CFTase was performed from the cell‐free culture of strain LX16 with a specific activity of 2.4 U mg−1, and the results were summarized in Table 3. The preparation gave a total protein recovery of 2.6% in a purification fold of 13.9 and the final product had a specific activity of 33.3 U mg−1. The purified CFTase was analysed by both native PAGE and SDS/PAGE stained with silver staining (Fig. 5A). One single protein band with molecular weight of 101 kDa was observed on the native PAGE, but two bands with molecular weight of 56.2 kDa and 44.8 kDa were observed on the SDS/PAGE. When the enzymatic degradation products of inulin by the purified CFTase were assessed by HPLC (Fig. 5B), one single type of FOS molecule was detected in the process of inulin degradation. The optimum temperature and pH for CFTase activity was 45°C and 7.0 respectively. The CFTase was active at pH ranged from 5.5 to 9.5, and at temperatures up to 55°C.

Bottom Line: The predominant cellular fatty acids were anteiso-C15:0, C16:0 and anteiso-C17:0.The FOS was identified as a cyclofructooligosaccharide with a degree of polymerization of 6.Such homology in inulin degradation products may be beneficial for the functional FOS production.

View Article: PubMed Central - PubMed

Affiliation: School of Biological Engineering, Dalian Polytechnic University, Dalian, 116034, China.

No MeSH data available.


Related in: MedlinePlus