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A microscopy study of hyphal growth of Penicillium rubens on gypsum under dynamic humidity conditions.

van Laarhoven KA, Huinink HP, Adan OC - Microb Biotechnol (2016)

Bottom Line: It was found that hyphal tips ceased to extend when exposed to any tested decrease in RH.However, new hyphal growth always emerges, seemingly from the old mycelium, suggesting that this indoor fungus does not rely only on conidia to survive the humidity patterns considered.These findings are a fundamental step in unravelling the effect of RH on indoor fungal growth.

View Article: PubMed Central - PubMed

Affiliation: Department of Applied Physics, Eindhoven University of Technology, Eindhoven, the Netherlands.

No MeSH data available.


Related in: MedlinePlus

Average growth rates of hyphae growing on a gypsum substrate, before and after exposure to a period of RHmin with duration Δt of 24 h (A) or 1 h (B). RH = 97% before and after the period of low RH. Error bars represent the standard deviation in the individual results. Welch's one‐way ANOVA shows that the averages are significantly heterogeneous (F9,55 = 30, P = 2.7e‐18) for (A), (F9,43 = 14, P = 3.69e‐18) for (B). Averages with a different label (a, b,…) are statistically different (Games‐Howell post hoc tests, P = 0.05).
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mbt212357-fig-0005: Average growth rates of hyphae growing on a gypsum substrate, before and after exposure to a period of RHmin with duration Δt of 24 h (A) or 1 h (B). RH = 97% before and after the period of low RH. Error bars represent the standard deviation in the individual results. Welch's one‐way ANOVA shows that the averages are significantly heterogeneous (F9,55 = 30, P = 2.7e‐18) for (A), (F9,43 = 14, P = 3.69e‐18) for (B). Averages with a different label (a, b,…) are statistically different (Games‐Howell post hoc tests, P = 0.05).

Mentions: Figure 3 displays several time labelled images of growth on the samples during a typical experiment (Δt = 24 h, RHmin = 90%). The images are cropped frames of the recorded movies. A typical example of an extending hypha as observed in the movies is shown in Fig. 3A. Growth rates measured during the initial 96 h of steady‐state RH = 97% (Fig. 1) served as a reference point for each experiment, justified on the basis of comparison with the steady‐state experiments. The average of these initial growth rates is included per experiment in Fig. 5. Welch's one‐way ANOVA shows that the average initial growth rates are statistically heterogeneous (F10,49 = 5, P = 2.9e‐5). However, further analysis (Games‐Howell post hoc tests, P = 0.05) showed that the average initial growth rate of any experiment did not differ significantly from the average growth rate during steady‐state growth at RH = 97%, which was 8 ± 3 μm h−1.


A microscopy study of hyphal growth of Penicillium rubens on gypsum under dynamic humidity conditions.

van Laarhoven KA, Huinink HP, Adan OC - Microb Biotechnol (2016)

Average growth rates of hyphae growing on a gypsum substrate, before and after exposure to a period of RHmin with duration Δt of 24 h (A) or 1 h (B). RH = 97% before and after the period of low RH. Error bars represent the standard deviation in the individual results. Welch's one‐way ANOVA shows that the averages are significantly heterogeneous (F9,55 = 30, P = 2.7e‐18) for (A), (F9,43 = 14, P = 3.69e‐18) for (B). Averages with a different label (a, b,…) are statistically different (Games‐Howell post hoc tests, P = 0.05).
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mbt212357-fig-0005: Average growth rates of hyphae growing on a gypsum substrate, before and after exposure to a period of RHmin with duration Δt of 24 h (A) or 1 h (B). RH = 97% before and after the period of low RH. Error bars represent the standard deviation in the individual results. Welch's one‐way ANOVA shows that the averages are significantly heterogeneous (F9,55 = 30, P = 2.7e‐18) for (A), (F9,43 = 14, P = 3.69e‐18) for (B). Averages with a different label (a, b,…) are statistically different (Games‐Howell post hoc tests, P = 0.05).
Mentions: Figure 3 displays several time labelled images of growth on the samples during a typical experiment (Δt = 24 h, RHmin = 90%). The images are cropped frames of the recorded movies. A typical example of an extending hypha as observed in the movies is shown in Fig. 3A. Growth rates measured during the initial 96 h of steady‐state RH = 97% (Fig. 1) served as a reference point for each experiment, justified on the basis of comparison with the steady‐state experiments. The average of these initial growth rates is included per experiment in Fig. 5. Welch's one‐way ANOVA shows that the average initial growth rates are statistically heterogeneous (F10,49 = 5, P = 2.9e‐5). However, further analysis (Games‐Howell post hoc tests, P = 0.05) showed that the average initial growth rate of any experiment did not differ significantly from the average growth rate during steady‐state growth at RH = 97%, which was 8 ± 3 μm h−1.

Bottom Line: It was found that hyphal tips ceased to extend when exposed to any tested decrease in RH.However, new hyphal growth always emerges, seemingly from the old mycelium, suggesting that this indoor fungus does not rely only on conidia to survive the humidity patterns considered.These findings are a fundamental step in unravelling the effect of RH on indoor fungal growth.

View Article: PubMed Central - PubMed

Affiliation: Department of Applied Physics, Eindhoven University of Technology, Eindhoven, the Netherlands.

No MeSH data available.


Related in: MedlinePlus