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A microscopy study of hyphal growth of Penicillium rubens on gypsum under dynamic humidity conditions.

van Laarhoven KA, Huinink HP, Adan OC - Microb Biotechnol (2016)

Bottom Line: It was found that hyphal tips ceased to extend when exposed to any tested decrease in RH.However, new hyphal growth always emerges, seemingly from the old mycelium, suggesting that this indoor fungus does not rely only on conidia to survive the humidity patterns considered.These findings are a fundamental step in unravelling the effect of RH on indoor fungal growth.

View Article: PubMed Central - PubMed

Affiliation: Department of Applied Physics, Eindhoven University of Technology, Eindhoven, the Netherlands.

No MeSH data available.


Related in: MedlinePlus

Hyphal length as a function of time for hyphae growing on gypsum samples while exposed to a single period of lowered RH with Δt = 24 h, RHmin = 90% (a) and Δt = 1 h, RHmin = 60% (B). Per figure, the data were obtained from tracing hyphae in two movies of growth on samples exposed to the low RH period. The dotted lines indicate the period of exposure to RHmin. The growth rate and moment of appearance of each individual hypha are determined with a linear fit, as illustrated with the dashed line in (A). t = 0 corresponds to the moment of inoculation.
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mbt212357-fig-0004: Hyphal length as a function of time for hyphae growing on gypsum samples while exposed to a single period of lowered RH with Δt = 24 h, RHmin = 90% (a) and Δt = 1 h, RHmin = 60% (B). Per figure, the data were obtained from tracing hyphae in two movies of growth on samples exposed to the low RH period. The dotted lines indicate the period of exposure to RHmin. The growth rate and moment of appearance of each individual hypha are determined with a linear fit, as illustrated with the dashed line in (A). t = 0 corresponds to the moment of inoculation.

Mentions: The recorded movies were post‐processed to quantify the observed hyphal growth. A custom MATLAB script was used to trace the position of individual hyphal tips from series of frames like those shown in Fig. 3A. Hyphal length as a function of time, starting from the first sight of growth, could be determined accordingly. An example of such data is displayed in Fig. 4, which shows the length of 37 individual hyphae that were traced from two movies of a full measurement, plotted as a function of time with t = 0 the moment of inoculation. The dotted lines mark the period of low RH.


A microscopy study of hyphal growth of Penicillium rubens on gypsum under dynamic humidity conditions.

van Laarhoven KA, Huinink HP, Adan OC - Microb Biotechnol (2016)

Hyphal length as a function of time for hyphae growing on gypsum samples while exposed to a single period of lowered RH with Δt = 24 h, RHmin = 90% (a) and Δt = 1 h, RHmin = 60% (B). Per figure, the data were obtained from tracing hyphae in two movies of growth on samples exposed to the low RH period. The dotted lines indicate the period of exposure to RHmin. The growth rate and moment of appearance of each individual hypha are determined with a linear fit, as illustrated with the dashed line in (A). t = 0 corresponds to the moment of inoculation.
© Copyright Policy - creativeCommonsBy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4835577&req=5

mbt212357-fig-0004: Hyphal length as a function of time for hyphae growing on gypsum samples while exposed to a single period of lowered RH with Δt = 24 h, RHmin = 90% (a) and Δt = 1 h, RHmin = 60% (B). Per figure, the data were obtained from tracing hyphae in two movies of growth on samples exposed to the low RH period. The dotted lines indicate the period of exposure to RHmin. The growth rate and moment of appearance of each individual hypha are determined with a linear fit, as illustrated with the dashed line in (A). t = 0 corresponds to the moment of inoculation.
Mentions: The recorded movies were post‐processed to quantify the observed hyphal growth. A custom MATLAB script was used to trace the position of individual hyphal tips from series of frames like those shown in Fig. 3A. Hyphal length as a function of time, starting from the first sight of growth, could be determined accordingly. An example of such data is displayed in Fig. 4, which shows the length of 37 individual hyphae that were traced from two movies of a full measurement, plotted as a function of time with t = 0 the moment of inoculation. The dotted lines mark the period of low RH.

Bottom Line: It was found that hyphal tips ceased to extend when exposed to any tested decrease in RH.However, new hyphal growth always emerges, seemingly from the old mycelium, suggesting that this indoor fungus does not rely only on conidia to survive the humidity patterns considered.These findings are a fundamental step in unravelling the effect of RH on indoor fungal growth.

View Article: PubMed Central - PubMed

Affiliation: Department of Applied Physics, Eindhoven University of Technology, Eindhoven, the Netherlands.

No MeSH data available.


Related in: MedlinePlus