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A microscopy study of hyphal growth of Penicillium rubens on gypsum under dynamic humidity conditions.

van Laarhoven KA, Huinink HP, Adan OC - Microb Biotechnol (2016)

Bottom Line: It was found that hyphal tips ceased to extend when exposed to any tested decrease in RH.However, new hyphal growth always emerges, seemingly from the old mycelium, suggesting that this indoor fungus does not rely only on conidia to survive the humidity patterns considered.These findings are a fundamental step in unravelling the effect of RH on indoor fungal growth.

View Article: PubMed Central - PubMed

Affiliation: Department of Applied Physics, Eindhoven University of Technology, Eindhoven, the Netherlands.

No MeSH data available.


Related in: MedlinePlus

Cropped frames of a typical movie produced with the video setup. The scale bars represent 25 μm. (A) A hypha growing on a gypsum sample exposed to RH = 97%, at 2 h intervals. The arrow marks the moment right before the hypha becomes visible. (B) A hypha severely displaces right after a change in RH from 97% to 90%. (C) Formation of a P. rubens fruiting body at 8 h intervals. The arrow marks the moment of sporulation, defined as the last frame in which no conidiophores can be recognized.
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mbt212357-fig-0003: Cropped frames of a typical movie produced with the video setup. The scale bars represent 25 μm. (A) A hypha growing on a gypsum sample exposed to RH = 97%, at 2 h intervals. The arrow marks the moment right before the hypha becomes visible. (B) A hypha severely displaces right after a change in RH from 97% to 90%. (C) Formation of a P. rubens fruiting body at 8 h intervals. The arrow marks the moment of sporulation, defined as the last frame in which no conidiophores can be recognized.

Mentions: The recorded movies were post‐processed to quantify the observed hyphal growth. A custom MATLAB script was used to trace the position of individual hyphal tips from series of frames like those shown in Fig. 3A. Hyphal length as a function of time, starting from the first sight of growth, could be determined accordingly. An example of such data is displayed in Fig. 4, which shows the length of 37 individual hyphae that were traced from two movies of a full measurement, plotted as a function of time with t = 0 the moment of inoculation. The dotted lines mark the period of low RH.


A microscopy study of hyphal growth of Penicillium rubens on gypsum under dynamic humidity conditions.

van Laarhoven KA, Huinink HP, Adan OC - Microb Biotechnol (2016)

Cropped frames of a typical movie produced with the video setup. The scale bars represent 25 μm. (A) A hypha growing on a gypsum sample exposed to RH = 97%, at 2 h intervals. The arrow marks the moment right before the hypha becomes visible. (B) A hypha severely displaces right after a change in RH from 97% to 90%. (C) Formation of a P. rubens fruiting body at 8 h intervals. The arrow marks the moment of sporulation, defined as the last frame in which no conidiophores can be recognized.
© Copyright Policy - creativeCommonsBy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4835577&req=5

mbt212357-fig-0003: Cropped frames of a typical movie produced with the video setup. The scale bars represent 25 μm. (A) A hypha growing on a gypsum sample exposed to RH = 97%, at 2 h intervals. The arrow marks the moment right before the hypha becomes visible. (B) A hypha severely displaces right after a change in RH from 97% to 90%. (C) Formation of a P. rubens fruiting body at 8 h intervals. The arrow marks the moment of sporulation, defined as the last frame in which no conidiophores can be recognized.
Mentions: The recorded movies were post‐processed to quantify the observed hyphal growth. A custom MATLAB script was used to trace the position of individual hyphal tips from series of frames like those shown in Fig. 3A. Hyphal length as a function of time, starting from the first sight of growth, could be determined accordingly. An example of such data is displayed in Fig. 4, which shows the length of 37 individual hyphae that were traced from two movies of a full measurement, plotted as a function of time with t = 0 the moment of inoculation. The dotted lines mark the period of low RH.

Bottom Line: It was found that hyphal tips ceased to extend when exposed to any tested decrease in RH.However, new hyphal growth always emerges, seemingly from the old mycelium, suggesting that this indoor fungus does not rely only on conidia to survive the humidity patterns considered.These findings are a fundamental step in unravelling the effect of RH on indoor fungal growth.

View Article: PubMed Central - PubMed

Affiliation: Department of Applied Physics, Eindhoven University of Technology, Eindhoven, the Netherlands.

No MeSH data available.


Related in: MedlinePlus