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Food borne bacterial models for detection of benzo[a]pyrene-DNA adducts formation using RAPD-PCR.

Lanzone V, Tofalo R, Fasoli G, Perpetuini G, Suzzi G, Sergi M, Corrado F, Compagnone D - Microb Biotechnol (2016)

Bottom Line: Some extra bands were detected for L. plantarum and E. faecium, while in E. coli and S. cerevisiae DNAs BPDE and DEB treatments did not result in new extra bands.Besides qualitatively analysis, cluster analysis based on Unweighted Pair-Group Method with Average algorithm was performed to compare DNA fingerprints before and after treatments.This analysis confirmed the absence of significant differences between negative controls and treated DNA in S. cerevisiae and E. coli however the disappearance of some bands can be detected.

View Article: PubMed Central - PubMed

Affiliation: Faculty of BioScience and Technology for Food, Agriculture and Environment, University of Teramo, Mosciano Sant'Angelo (TE), 64023, Italy.

No MeSH data available.


Related in: MedlinePlus

RAPD fingerprinting for Saccharomyces cerevisiae S441 strain with LA1 primer obtained for untreated and treated DNA samples using different concentrations of BPDE (0.01–0.1–1 μM). M: 1 kb DNA Plus ladder (Life Technologies (Monza, Italy)), n.c.: negative control.
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mbt212355-fig-0001: RAPD fingerprinting for Saccharomyces cerevisiae S441 strain with LA1 primer obtained for untreated and treated DNA samples using different concentrations of BPDE (0.01–0.1–1 μM). M: 1 kb DNA Plus ladder (Life Technologies (Monza, Italy)), n.c.: negative control.

Mentions: The activated form of benzo(a)pyrene (BPDE) was used to test the reactivity versus DNA. LA1 primer was used to investigate the effects on RAPD profile of S. cerevisiae treated at three different concentrations of BPDE (0.01–0.1–1 μM). Using low concentrations of BPDE (0.01–0.1 μM) LA1 fingerprintings of treated DNA and negative controls were similar (Fig. 1). Minor modifications were obtained when the concentration was 1 μM; in particular, bands with higher molecular weight disappeared. Similar results were obtained for bacteria suggesting that BDPE acts in a dose dependent manner (data not shown).


Food borne bacterial models for detection of benzo[a]pyrene-DNA adducts formation using RAPD-PCR.

Lanzone V, Tofalo R, Fasoli G, Perpetuini G, Suzzi G, Sergi M, Corrado F, Compagnone D - Microb Biotechnol (2016)

RAPD fingerprinting for Saccharomyces cerevisiae S441 strain with LA1 primer obtained for untreated and treated DNA samples using different concentrations of BPDE (0.01–0.1–1 μM). M: 1 kb DNA Plus ladder (Life Technologies (Monza, Italy)), n.c.: negative control.
© Copyright Policy - creativeCommonsBy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4835576&req=5

mbt212355-fig-0001: RAPD fingerprinting for Saccharomyces cerevisiae S441 strain with LA1 primer obtained for untreated and treated DNA samples using different concentrations of BPDE (0.01–0.1–1 μM). M: 1 kb DNA Plus ladder (Life Technologies (Monza, Italy)), n.c.: negative control.
Mentions: The activated form of benzo(a)pyrene (BPDE) was used to test the reactivity versus DNA. LA1 primer was used to investigate the effects on RAPD profile of S. cerevisiae treated at three different concentrations of BPDE (0.01–0.1–1 μM). Using low concentrations of BPDE (0.01–0.1 μM) LA1 fingerprintings of treated DNA and negative controls were similar (Fig. 1). Minor modifications were obtained when the concentration was 1 μM; in particular, bands with higher molecular weight disappeared. Similar results were obtained for bacteria suggesting that BDPE acts in a dose dependent manner (data not shown).

Bottom Line: Some extra bands were detected for L. plantarum and E. faecium, while in E. coli and S. cerevisiae DNAs BPDE and DEB treatments did not result in new extra bands.Besides qualitatively analysis, cluster analysis based on Unweighted Pair-Group Method with Average algorithm was performed to compare DNA fingerprints before and after treatments.This analysis confirmed the absence of significant differences between negative controls and treated DNA in S. cerevisiae and E. coli however the disappearance of some bands can be detected.

View Article: PubMed Central - PubMed

Affiliation: Faculty of BioScience and Technology for Food, Agriculture and Environment, University of Teramo, Mosciano Sant'Angelo (TE), 64023, Italy.

No MeSH data available.


Related in: MedlinePlus