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Growing media constituents determine the microbial nitrogen conversions in organic growing media for horticulture.

Grunert O, Reheul D, Van Labeke MC, Perneel M, Hernandez-Sanabria E, Vlaeminck SE, Boon N - Microb Biotechnol (2016)

Bottom Line: Interestingly, mixing of the growing media constituents resulted in a stimulation of the function of the microorganisms.The use of organic fertilizer resulted in an increase in amoA gene copy number by factor 100 compared to inorganic fertilizers.Our results support our hypothesis that the activity of the functional microbial community with respect to nitrogen turnover in an organic growing medium can be improved by selecting and mixing the appropriate growing media components with each other.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Microbial Ecology and Technology (LabMET), Ghent University, Coupure Links 653, Gent, 9000, Belgium.

No MeSH data available.


Changes in abundance of bacterial amoA genes and total bacteria organic growing media (GB) in combination with inorganic fertilizer (IF) and organic fertilizer (OF). Bars represent standard deviation of triplicate samples. Different letters above the bars indicate a significant difference (P < 0.05). GBOF1: organic growing medium with organic fertilizer (0 ml air s−1); GBOF2: organic growing medium with organic fertilizer (17 ml air s−1); GBOF3: organic growing medium with organic fertilizer (25 ml air s−1).
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mbt212354-fig-0002: Changes in abundance of bacterial amoA genes and total bacteria organic growing media (GB) in combination with inorganic fertilizer (IF) and organic fertilizer (OF). Bars represent standard deviation of triplicate samples. Different letters above the bars indicate a significant difference (P < 0.05). GBOF1: organic growing medium with organic fertilizer (0 ml air s−1); GBOF2: organic growing medium with organic fertilizer (17 ml air s−1); GBOF3: organic growing medium with organic fertilizer (25 ml air s−1).

Mentions: Figure 2 shows the changes in abundance of bacterial amoA genes of the organic growing medium treated with organic or inorganic fertilizer. We determined that the AOB amoA gene copy number was below detection limit in both fresh growing media at the start of the test. The copy number of the total 16S rRNA genes was below the detection limit (<103 copies g−1 of growing medium) for the mineral media (RW). For the mixture we found 1.5 × 105 ± 2.3 × 104 copies per g of the mixture (GB). Growing media with different fertilizer regimes were also analysed after 55 days by qPCR. The percentage of the bacterial copy number of amoA gene over de total 16S rRNA gene copy number was 0.12% in the GBIF treatment, 1.17% in the GBOF1, 1.02% in the GBOF2 and 0.90% in the GBOF3 treatment. Blowing air into the growing medium and the use of organic fertilizer had a significant positive effect on the bacterial amoA gene copy number (P < 0.001) and the total bacteria (P < 0.001), however, the ratio amoA gene copy number over total 16S rRNA gene copy number remained stable with increasing air flow.


Growing media constituents determine the microbial nitrogen conversions in organic growing media for horticulture.

Grunert O, Reheul D, Van Labeke MC, Perneel M, Hernandez-Sanabria E, Vlaeminck SE, Boon N - Microb Biotechnol (2016)

Changes in abundance of bacterial amoA genes and total bacteria organic growing media (GB) in combination with inorganic fertilizer (IF) and organic fertilizer (OF). Bars represent standard deviation of triplicate samples. Different letters above the bars indicate a significant difference (P < 0.05). GBOF1: organic growing medium with organic fertilizer (0 ml air s−1); GBOF2: organic growing medium with organic fertilizer (17 ml air s−1); GBOF3: organic growing medium with organic fertilizer (25 ml air s−1).
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Related In: Results  -  Collection

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mbt212354-fig-0002: Changes in abundance of bacterial amoA genes and total bacteria organic growing media (GB) in combination with inorganic fertilizer (IF) and organic fertilizer (OF). Bars represent standard deviation of triplicate samples. Different letters above the bars indicate a significant difference (P < 0.05). GBOF1: organic growing medium with organic fertilizer (0 ml air s−1); GBOF2: organic growing medium with organic fertilizer (17 ml air s−1); GBOF3: organic growing medium with organic fertilizer (25 ml air s−1).
Mentions: Figure 2 shows the changes in abundance of bacterial amoA genes of the organic growing medium treated with organic or inorganic fertilizer. We determined that the AOB amoA gene copy number was below detection limit in both fresh growing media at the start of the test. The copy number of the total 16S rRNA genes was below the detection limit (<103 copies g−1 of growing medium) for the mineral media (RW). For the mixture we found 1.5 × 105 ± 2.3 × 104 copies per g of the mixture (GB). Growing media with different fertilizer regimes were also analysed after 55 days by qPCR. The percentage of the bacterial copy number of amoA gene over de total 16S rRNA gene copy number was 0.12% in the GBIF treatment, 1.17% in the GBOF1, 1.02% in the GBOF2 and 0.90% in the GBOF3 treatment. Blowing air into the growing medium and the use of organic fertilizer had a significant positive effect on the bacterial amoA gene copy number (P < 0.001) and the total bacteria (P < 0.001), however, the ratio amoA gene copy number over total 16S rRNA gene copy number remained stable with increasing air flow.

Bottom Line: Interestingly, mixing of the growing media constituents resulted in a stimulation of the function of the microorganisms.The use of organic fertilizer resulted in an increase in amoA gene copy number by factor 100 compared to inorganic fertilizers.Our results support our hypothesis that the activity of the functional microbial community with respect to nitrogen turnover in an organic growing medium can be improved by selecting and mixing the appropriate growing media components with each other.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Microbial Ecology and Technology (LabMET), Ghent University, Coupure Links 653, Gent, 9000, Belgium.

No MeSH data available.