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Phenotypic and Molecular Assessment of Drug Resistance Profile and Genetic Diversity of Waterborne Escherichia coli.

Wolny-Koładka K, Lenart-Boroń A - Water Air Soil Pollut (2016)

Bottom Line: Numerous multi-drug resistant strains were detected, which however did not exhibit the ESBL phenotype.However, PCR approach allowed to detect ESBL-mechanism genes (CTX-M3, OXA, SHV, and TEM) in as much as 38 % of E. coli isolates.These results were coupled with significant molecular diversity of the E. coli strains revealed in BOXA1R-based rep-PCR technique.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology, University of Agriculture in Cracow, Mickiewicza Ave 24/28, 30-059 Cracow, Poland.

ABSTRACT

Bacterial Escherichia coli isolates were derived from waters of the Nowohucki Reservoir (Cracow, Poland) in summer and winter seasons. In total, 94 strains, identified as E. coli, were isolated from five sampling sites in the area of the reservoir. Based on the disk-diffusion tests, it was found that the tested isolates were predominantly resistant to ticarcillin and ampicillin. Numerous multi-drug resistant strains were detected, which however did not exhibit the ESBL phenotype. However, PCR approach allowed to detect ESBL-mechanism genes (CTX-M3, OXA, SHV, and TEM) in as much as 38 % of E. coli isolates. These results were coupled with significant molecular diversity of the E. coli strains revealed in BOXA1R-based rep-PCR technique.

No MeSH data available.


Related in: MedlinePlus

Prevalence of antibiotic resistance genes responsible for the emergence of ESBL mechanism in E. coli
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Fig6: Prevalence of antibiotic resistance genes responsible for the emergence of ESBL mechanism in E. coli

Mentions: Figure 6 shows the incidence of genes responsible for the production of extended spectrum β-lactamases (ESBL). The blaTEM gene was most frequently found (occurred in 27 isolates), while blaCTX-M9 was not detected in any of the analyzed strains. In most cases, the analyzed genes occurred separately in the E. coli strains, only in two isolates we found a co-occurrence of two genes, i.e., blaOXA with blaTEM and in another two—blaSHV with blaTEM. Moreover, the presence of blaTEM only was found in bacteria isolated in the winter, while the remaining genes were present in E. coli strains isolated in the summer.Fig. 6


Phenotypic and Molecular Assessment of Drug Resistance Profile and Genetic Diversity of Waterborne Escherichia coli.

Wolny-Koładka K, Lenart-Boroń A - Water Air Soil Pollut (2016)

Prevalence of antibiotic resistance genes responsible for the emergence of ESBL mechanism in E. coli
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4835524&req=5

Fig6: Prevalence of antibiotic resistance genes responsible for the emergence of ESBL mechanism in E. coli
Mentions: Figure 6 shows the incidence of genes responsible for the production of extended spectrum β-lactamases (ESBL). The blaTEM gene was most frequently found (occurred in 27 isolates), while blaCTX-M9 was not detected in any of the analyzed strains. In most cases, the analyzed genes occurred separately in the E. coli strains, only in two isolates we found a co-occurrence of two genes, i.e., blaOXA with blaTEM and in another two—blaSHV with blaTEM. Moreover, the presence of blaTEM only was found in bacteria isolated in the winter, while the remaining genes were present in E. coli strains isolated in the summer.Fig. 6

Bottom Line: Numerous multi-drug resistant strains were detected, which however did not exhibit the ESBL phenotype.However, PCR approach allowed to detect ESBL-mechanism genes (CTX-M3, OXA, SHV, and TEM) in as much as 38 % of E. coli isolates.These results were coupled with significant molecular diversity of the E. coli strains revealed in BOXA1R-based rep-PCR technique.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology, University of Agriculture in Cracow, Mickiewicza Ave 24/28, 30-059 Cracow, Poland.

ABSTRACT

Bacterial Escherichia coli isolates were derived from waters of the Nowohucki Reservoir (Cracow, Poland) in summer and winter seasons. In total, 94 strains, identified as E. coli, were isolated from five sampling sites in the area of the reservoir. Based on the disk-diffusion tests, it was found that the tested isolates were predominantly resistant to ticarcillin and ampicillin. Numerous multi-drug resistant strains were detected, which however did not exhibit the ESBL phenotype. However, PCR approach allowed to detect ESBL-mechanism genes (CTX-M3, OXA, SHV, and TEM) in as much as 38 % of E. coli isolates. These results were coupled with significant molecular diversity of the E. coli strains revealed in BOXA1R-based rep-PCR technique.

No MeSH data available.


Related in: MedlinePlus