Limits...
Phenotypic and Molecular Assessment of Drug Resistance Profile and Genetic Diversity of Waterborne Escherichia coli.

Wolny-Koładka K, Lenart-Boroń A - Water Air Soil Pollut (2016)

Bottom Line: Numerous multi-drug resistant strains were detected, which however did not exhibit the ESBL phenotype.However, PCR approach allowed to detect ESBL-mechanism genes (CTX-M3, OXA, SHV, and TEM) in as much as 38 % of E. coli isolates.These results were coupled with significant molecular diversity of the E. coli strains revealed in BOXA1R-based rep-PCR technique.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology, University of Agriculture in Cracow, Mickiewicza Ave 24/28, 30-059 Cracow, Poland.

ABSTRACT

Bacterial Escherichia coli isolates were derived from waters of the Nowohucki Reservoir (Cracow, Poland) in summer and winter seasons. In total, 94 strains, identified as E. coli, were isolated from five sampling sites in the area of the reservoir. Based on the disk-diffusion tests, it was found that the tested isolates were predominantly resistant to ticarcillin and ampicillin. Numerous multi-drug resistant strains were detected, which however did not exhibit the ESBL phenotype. However, PCR approach allowed to detect ESBL-mechanism genes (CTX-M3, OXA, SHV, and TEM) in as much as 38 % of E. coli isolates. These results were coupled with significant molecular diversity of the E. coli strains revealed in BOXA1R-based rep-PCR technique.

No MeSH data available.


Related in: MedlinePlus

UPGMA dendrogram of waterborne E. coli strains collected in the winter from 5 points located within the area of the Nowohucki Reservoir
© Copyright Policy - OpenAccess
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4835524&req=5

Fig5: UPGMA dendrogram of waterborne E. coli strains collected in the winter from 5 points located within the area of the Nowohucki Reservoir

Mentions: Dendrograms (Figs. 4 and 5), which showed the results of analyses aiming at determining most closely related isolates, as well as verification of their potential relationship with the reference strain, were constructed based on the rep-PCR analyses (BOX-PCR). The figures show significant diversity between E. coli isolates. Based on these dendrograms, it can be concluded that the examined isolates are characterized by large genetic diversity and they cannot be easily grouped into clusters. Some clusters are composed of isolates derived from individual sampling sites, while others comprise isolates from various sites. Also, the position of a reference strain ATCC 25922 does not allow to demonstrate significant differences between the clusters obtained. The analysis of the presented dendrograms allows to conclude that the E. coli strains, even though originate from a relatively isolated environment, such as the tested reservoir, are very diverse. The overall genetic variation between and within groups (summer and winter) according to AMOVA principles was presented in Table 4.Fig. 4


Phenotypic and Molecular Assessment of Drug Resistance Profile and Genetic Diversity of Waterborne Escherichia coli.

Wolny-Koładka K, Lenart-Boroń A - Water Air Soil Pollut (2016)

UPGMA dendrogram of waterborne E. coli strains collected in the winter from 5 points located within the area of the Nowohucki Reservoir
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4835524&req=5

Fig5: UPGMA dendrogram of waterborne E. coli strains collected in the winter from 5 points located within the area of the Nowohucki Reservoir
Mentions: Dendrograms (Figs. 4 and 5), which showed the results of analyses aiming at determining most closely related isolates, as well as verification of their potential relationship with the reference strain, were constructed based on the rep-PCR analyses (BOX-PCR). The figures show significant diversity between E. coli isolates. Based on these dendrograms, it can be concluded that the examined isolates are characterized by large genetic diversity and they cannot be easily grouped into clusters. Some clusters are composed of isolates derived from individual sampling sites, while others comprise isolates from various sites. Also, the position of a reference strain ATCC 25922 does not allow to demonstrate significant differences between the clusters obtained. The analysis of the presented dendrograms allows to conclude that the E. coli strains, even though originate from a relatively isolated environment, such as the tested reservoir, are very diverse. The overall genetic variation between and within groups (summer and winter) according to AMOVA principles was presented in Table 4.Fig. 4

Bottom Line: Numerous multi-drug resistant strains were detected, which however did not exhibit the ESBL phenotype.However, PCR approach allowed to detect ESBL-mechanism genes (CTX-M3, OXA, SHV, and TEM) in as much as 38 % of E. coli isolates.These results were coupled with significant molecular diversity of the E. coli strains revealed in BOXA1R-based rep-PCR technique.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology, University of Agriculture in Cracow, Mickiewicza Ave 24/28, 30-059 Cracow, Poland.

ABSTRACT

Bacterial Escherichia coli isolates were derived from waters of the Nowohucki Reservoir (Cracow, Poland) in summer and winter seasons. In total, 94 strains, identified as E. coli, were isolated from five sampling sites in the area of the reservoir. Based on the disk-diffusion tests, it was found that the tested isolates were predominantly resistant to ticarcillin and ampicillin. Numerous multi-drug resistant strains were detected, which however did not exhibit the ESBL phenotype. However, PCR approach allowed to detect ESBL-mechanism genes (CTX-M3, OXA, SHV, and TEM) in as much as 38 % of E. coli isolates. These results were coupled with significant molecular diversity of the E. coli strains revealed in BOXA1R-based rep-PCR technique.

No MeSH data available.


Related in: MedlinePlus