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Phenotypic and Molecular Assessment of Drug Resistance Profile and Genetic Diversity of Waterborne Escherichia coli.

Wolny-Koładka K, Lenart-Boroń A - Water Air Soil Pollut (2016)

Bottom Line: Numerous multi-drug resistant strains were detected, which however did not exhibit the ESBL phenotype.However, PCR approach allowed to detect ESBL-mechanism genes (CTX-M3, OXA, SHV, and TEM) in as much as 38 % of E. coli isolates.These results were coupled with significant molecular diversity of the E. coli strains revealed in BOXA1R-based rep-PCR technique.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology, University of Agriculture in Cracow, Mickiewicza Ave 24/28, 30-059 Cracow, Poland.

ABSTRACT

Bacterial Escherichia coli isolates were derived from waters of the Nowohucki Reservoir (Cracow, Poland) in summer and winter seasons. In total, 94 strains, identified as E. coli, were isolated from five sampling sites in the area of the reservoir. Based on the disk-diffusion tests, it was found that the tested isolates were predominantly resistant to ticarcillin and ampicillin. Numerous multi-drug resistant strains were detected, which however did not exhibit the ESBL phenotype. However, PCR approach allowed to detect ESBL-mechanism genes (CTX-M3, OXA, SHV, and TEM) in as much as 38 % of E. coli isolates. These results were coupled with significant molecular diversity of the E. coli strains revealed in BOXA1R-based rep-PCR technique.

No MeSH data available.


Related in: MedlinePlus

Changes in the waterborne E. coli antimicrobial resistance profile according to the period of strain isolation
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Related In: Results  -  Collection


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Fig3: Changes in the waterborne E. coli antimicrobial resistance profile according to the period of strain isolation

Mentions: The analysis of antimicrobial resistance profile of waterborne E. coli isolated from the Nowohucki Reservoir indicated that the strains collected during the summer are characterized by much higher resistance to the tested drugs (Fig. 3).Fig. 3


Phenotypic and Molecular Assessment of Drug Resistance Profile and Genetic Diversity of Waterborne Escherichia coli.

Wolny-Koładka K, Lenart-Boroń A - Water Air Soil Pollut (2016)

Changes in the waterborne E. coli antimicrobial resistance profile according to the period of strain isolation
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4835524&req=5

Fig3: Changes in the waterborne E. coli antimicrobial resistance profile according to the period of strain isolation
Mentions: The analysis of antimicrobial resistance profile of waterborne E. coli isolated from the Nowohucki Reservoir indicated that the strains collected during the summer are characterized by much higher resistance to the tested drugs (Fig. 3).Fig. 3

Bottom Line: Numerous multi-drug resistant strains were detected, which however did not exhibit the ESBL phenotype.However, PCR approach allowed to detect ESBL-mechanism genes (CTX-M3, OXA, SHV, and TEM) in as much as 38 % of E. coli isolates.These results were coupled with significant molecular diversity of the E. coli strains revealed in BOXA1R-based rep-PCR technique.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology, University of Agriculture in Cracow, Mickiewicza Ave 24/28, 30-059 Cracow, Poland.

ABSTRACT

Bacterial Escherichia coli isolates were derived from waters of the Nowohucki Reservoir (Cracow, Poland) in summer and winter seasons. In total, 94 strains, identified as E. coli, were isolated from five sampling sites in the area of the reservoir. Based on the disk-diffusion tests, it was found that the tested isolates were predominantly resistant to ticarcillin and ampicillin. Numerous multi-drug resistant strains were detected, which however did not exhibit the ESBL phenotype. However, PCR approach allowed to detect ESBL-mechanism genes (CTX-M3, OXA, SHV, and TEM) in as much as 38 % of E. coli isolates. These results were coupled with significant molecular diversity of the E. coli strains revealed in BOXA1R-based rep-PCR technique.

No MeSH data available.


Related in: MedlinePlus