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Phenotypic and Molecular Assessment of Drug Resistance Profile and Genetic Diversity of Waterborne Escherichia coli.

Wolny-Koładka K, Lenart-Boroń A - Water Air Soil Pollut (2016)

Bottom Line: Numerous multi-drug resistant strains were detected, which however did not exhibit the ESBL phenotype.However, PCR approach allowed to detect ESBL-mechanism genes (CTX-M3, OXA, SHV, and TEM) in as much as 38 % of E. coli isolates.These results were coupled with significant molecular diversity of the E. coli strains revealed in BOXA1R-based rep-PCR technique.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology, University of Agriculture in Cracow, Mickiewicza Ave 24/28, 30-059 Cracow, Poland.

ABSTRACT

Bacterial Escherichia coli isolates were derived from waters of the Nowohucki Reservoir (Cracow, Poland) in summer and winter seasons. In total, 94 strains, identified as E. coli, were isolated from five sampling sites in the area of the reservoir. Based on the disk-diffusion tests, it was found that the tested isolates were predominantly resistant to ticarcillin and ampicillin. Numerous multi-drug resistant strains were detected, which however did not exhibit the ESBL phenotype. However, PCR approach allowed to detect ESBL-mechanism genes (CTX-M3, OXA, SHV, and TEM) in as much as 38 % of E. coli isolates. These results were coupled with significant molecular diversity of the E. coli strains revealed in BOXA1R-based rep-PCR technique.

No MeSH data available.


Related in: MedlinePlus

Location of water sampling sites (www.mapy.geoportal.gov.pl/imap/)
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Related In: Results  -  Collection


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Fig1: Location of water sampling sites (www.mapy.geoportal.gov.pl/imap/)

Mentions: The water samples were collected from five points situated within the Nowohucki Reservoir, located in Nowa Huta—the largest district of Cracow (Poland). The samples were taken into sterile containers, twice per year on the following dates: January 15th 2015 (winter) and August 8th 2015 (summer). The collection points are presented in Fig. 1: site 1—water inflow; sites 2, 3, and 4—characteristic sites along the reservoirs banks; and 5—outflow. Water and air temperature were measured onsite during sampling using an electronic thermometer (Biowin). The isolation of E. coli was conducted by membrane filtration method on ENDO agar (BTL, Poland). The cultures were incubated at 44 °C for 48 h to isolate thermotolerant (fecal) E. coli (purple colonies with metallic sheen).Fig. 1


Phenotypic and Molecular Assessment of Drug Resistance Profile and Genetic Diversity of Waterborne Escherichia coli.

Wolny-Koładka K, Lenart-Boroń A - Water Air Soil Pollut (2016)

Location of water sampling sites (www.mapy.geoportal.gov.pl/imap/)
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4835524&req=5

Fig1: Location of water sampling sites (www.mapy.geoportal.gov.pl/imap/)
Mentions: The water samples were collected from five points situated within the Nowohucki Reservoir, located in Nowa Huta—the largest district of Cracow (Poland). The samples were taken into sterile containers, twice per year on the following dates: January 15th 2015 (winter) and August 8th 2015 (summer). The collection points are presented in Fig. 1: site 1—water inflow; sites 2, 3, and 4—characteristic sites along the reservoirs banks; and 5—outflow. Water and air temperature were measured onsite during sampling using an electronic thermometer (Biowin). The isolation of E. coli was conducted by membrane filtration method on ENDO agar (BTL, Poland). The cultures were incubated at 44 °C for 48 h to isolate thermotolerant (fecal) E. coli (purple colonies with metallic sheen).Fig. 1

Bottom Line: Numerous multi-drug resistant strains were detected, which however did not exhibit the ESBL phenotype.However, PCR approach allowed to detect ESBL-mechanism genes (CTX-M3, OXA, SHV, and TEM) in as much as 38 % of E. coli isolates.These results were coupled with significant molecular diversity of the E. coli strains revealed in BOXA1R-based rep-PCR technique.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology, University of Agriculture in Cracow, Mickiewicza Ave 24/28, 30-059 Cracow, Poland.

ABSTRACT

Bacterial Escherichia coli isolates were derived from waters of the Nowohucki Reservoir (Cracow, Poland) in summer and winter seasons. In total, 94 strains, identified as E. coli, were isolated from five sampling sites in the area of the reservoir. Based on the disk-diffusion tests, it was found that the tested isolates were predominantly resistant to ticarcillin and ampicillin. Numerous multi-drug resistant strains were detected, which however did not exhibit the ESBL phenotype. However, PCR approach allowed to detect ESBL-mechanism genes (CTX-M3, OXA, SHV, and TEM) in as much as 38 % of E. coli isolates. These results were coupled with significant molecular diversity of the E. coli strains revealed in BOXA1R-based rep-PCR technique.

No MeSH data available.


Related in: MedlinePlus