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Microencapsulation in Alginate and Chitosan Microgels to Enhance Viability of Bifidobacterium longum for Oral Delivery.

Yeung TW, Üçok EF, Tiani KA, McClements DJ, Sela DA - Front Microbiol (2016)

Bottom Line: Encapsulated obligate anaerobes Bifidobacterium longum subsp. infantis and Bifidobacterium longum subsp. longum exhibited differences in viability in a strain-dependent manner, without a discernable relationship to subspecies lineage.Coating alginate microgels with chitosan did not improve viability compared to cells encapsulated in alginate microgels alone, suggesting that modifying the surface charge alone does not enhance delivery.Thus hydrogel beads have great potential for improving the stability and efficacy of bifidobacterial probiotics in various nutritional interventions.

View Article: PubMed Central - PubMed

Affiliation: Department of Food Science, University of Massachusetts Amherst, MA, USA.

ABSTRACT
Probiotic microorganisms are incorporated into a wide variety of foods, supplements, and pharmaceuticals to promote human health and wellness. However, maintaining bacterial cell viability during storage and gastrointestinal transit remains a challenge. Encapsulation of bifidobacteria within food-grade hydrogel particles potentially mitigates their sensitivity to environmental stresses. In this study, Bifidobacterium longum subspecies and strains were encapsulated in core-shell microgels consisting of an alginate core and a microgel shell. Encapsulated obligate anaerobes Bifidobacterium longum subsp. infantis and Bifidobacterium longum subsp. longum exhibited differences in viability in a strain-dependent manner, without a discernable relationship to subspecies lineage. This includes viability under aerobic storage conditions and modeled gastrointestinal tract conditions. Coating alginate microgels with chitosan did not improve viability compared to cells encapsulated in alginate microgels alone, suggesting that modifying the surface charge alone does not enhance delivery. Thus hydrogel beads have great potential for improving the stability and efficacy of bifidobacterial probiotics in various nutritional interventions.

No MeSH data available.


Related in: MedlinePlus

Survival of (A) free B. longum cells, (B) B. longum cells in calcium alginate microbeads, (C) B. longum cells in chitosan-coated alginate microbeads in wet refrigerated storage over time. Counts based on samples drop-plated on MRS agar and incubated at 37°C anaerobically. Error bars indicate the standard error of replicate counts (n = 10).
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Figure 3: Survival of (A) free B. longum cells, (B) B. longum cells in calcium alginate microbeads, (C) B. longum cells in chitosan-coated alginate microbeads in wet refrigerated storage over time. Counts based on samples drop-plated on MRS agar and incubated at 37°C anaerobically. Error bars indicate the standard error of replicate counts (n = 10).

Mentions: The viability of four B. longum and four B. infantis strains that were not encapsulated was determined during 5 weeks of storage (Figure 3A; Supplementary Table S1). As expected, there was a decrease in the viability of the bifidobacteria evaluated, but the rate of the decrease was strain dependent. A sharp decrease in viability was observed for B. infantis UMA318 and B. longum UMA401, diminishing by 9–10 log CFU over the course of a week under aerobic conditions. B. infantis UMA 300 and B. infantis UMA 305 remained viable for slightly longer, with a 10-log reduction observed within 10 days. Whereas, B. infantis UMA 298 and B. infantis 306 exhibited a 9–10 log decrease over 2 weeks of storage. Interestingly, B. infantis UMA 299 and B. longum UMA 402 survived the longest, as viable cell counts diminished by 7–8 logs over 3 weeks before decreasing to undetectable levels.


Microencapsulation in Alginate and Chitosan Microgels to Enhance Viability of Bifidobacterium longum for Oral Delivery.

Yeung TW, Üçok EF, Tiani KA, McClements DJ, Sela DA - Front Microbiol (2016)

Survival of (A) free B. longum cells, (B) B. longum cells in calcium alginate microbeads, (C) B. longum cells in chitosan-coated alginate microbeads in wet refrigerated storage over time. Counts based on samples drop-plated on MRS agar and incubated at 37°C anaerobically. Error bars indicate the standard error of replicate counts (n = 10).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4835488&req=5

Figure 3: Survival of (A) free B. longum cells, (B) B. longum cells in calcium alginate microbeads, (C) B. longum cells in chitosan-coated alginate microbeads in wet refrigerated storage over time. Counts based on samples drop-plated on MRS agar and incubated at 37°C anaerobically. Error bars indicate the standard error of replicate counts (n = 10).
Mentions: The viability of four B. longum and four B. infantis strains that were not encapsulated was determined during 5 weeks of storage (Figure 3A; Supplementary Table S1). As expected, there was a decrease in the viability of the bifidobacteria evaluated, but the rate of the decrease was strain dependent. A sharp decrease in viability was observed for B. infantis UMA318 and B. longum UMA401, diminishing by 9–10 log CFU over the course of a week under aerobic conditions. B. infantis UMA 300 and B. infantis UMA 305 remained viable for slightly longer, with a 10-log reduction observed within 10 days. Whereas, B. infantis UMA 298 and B. infantis 306 exhibited a 9–10 log decrease over 2 weeks of storage. Interestingly, B. infantis UMA 299 and B. longum UMA 402 survived the longest, as viable cell counts diminished by 7–8 logs over 3 weeks before decreasing to undetectable levels.

Bottom Line: Encapsulated obligate anaerobes Bifidobacterium longum subsp. infantis and Bifidobacterium longum subsp. longum exhibited differences in viability in a strain-dependent manner, without a discernable relationship to subspecies lineage.Coating alginate microgels with chitosan did not improve viability compared to cells encapsulated in alginate microgels alone, suggesting that modifying the surface charge alone does not enhance delivery.Thus hydrogel beads have great potential for improving the stability and efficacy of bifidobacterial probiotics in various nutritional interventions.

View Article: PubMed Central - PubMed

Affiliation: Department of Food Science, University of Massachusetts Amherst, MA, USA.

ABSTRACT
Probiotic microorganisms are incorporated into a wide variety of foods, supplements, and pharmaceuticals to promote human health and wellness. However, maintaining bacterial cell viability during storage and gastrointestinal transit remains a challenge. Encapsulation of bifidobacteria within food-grade hydrogel particles potentially mitigates their sensitivity to environmental stresses. In this study, Bifidobacterium longum subspecies and strains were encapsulated in core-shell microgels consisting of an alginate core and a microgel shell. Encapsulated obligate anaerobes Bifidobacterium longum subsp. infantis and Bifidobacterium longum subsp. longum exhibited differences in viability in a strain-dependent manner, without a discernable relationship to subspecies lineage. This includes viability under aerobic storage conditions and modeled gastrointestinal tract conditions. Coating alginate microgels with chitosan did not improve viability compared to cells encapsulated in alginate microgels alone, suggesting that modifying the surface charge alone does not enhance delivery. Thus hydrogel beads have great potential for improving the stability and efficacy of bifidobacterial probiotics in various nutritional interventions.

No MeSH data available.


Related in: MedlinePlus