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Microencapsulation in Alginate and Chitosan Microgels to Enhance Viability of Bifidobacterium longum for Oral Delivery.

Yeung TW, Üçok EF, Tiani KA, McClements DJ, Sela DA - Front Microbiol (2016)

Bottom Line: Encapsulated obligate anaerobes Bifidobacterium longum subsp. infantis and Bifidobacterium longum subsp. longum exhibited differences in viability in a strain-dependent manner, without a discernable relationship to subspecies lineage.Coating alginate microgels with chitosan did not improve viability compared to cells encapsulated in alginate microgels alone, suggesting that modifying the surface charge alone does not enhance delivery.Thus hydrogel beads have great potential for improving the stability and efficacy of bifidobacterial probiotics in various nutritional interventions.

View Article: PubMed Central - PubMed

Affiliation: Department of Food Science, University of Massachusetts Amherst, MA, USA.

ABSTRACT
Probiotic microorganisms are incorporated into a wide variety of foods, supplements, and pharmaceuticals to promote human health and wellness. However, maintaining bacterial cell viability during storage and gastrointestinal transit remains a challenge. Encapsulation of bifidobacteria within food-grade hydrogel particles potentially mitigates their sensitivity to environmental stresses. In this study, Bifidobacterium longum subspecies and strains were encapsulated in core-shell microgels consisting of an alginate core and a microgel shell. Encapsulated obligate anaerobes Bifidobacterium longum subsp. infantis and Bifidobacterium longum subsp. longum exhibited differences in viability in a strain-dependent manner, without a discernable relationship to subspecies lineage. This includes viability under aerobic storage conditions and modeled gastrointestinal tract conditions. Coating alginate microgels with chitosan did not improve viability compared to cells encapsulated in alginate microgels alone, suggesting that modifying the surface charge alone does not enhance delivery. Thus hydrogel beads have great potential for improving the stability and efficacy of bifidobacterial probiotics in various nutritional interventions.

No MeSH data available.


Related in: MedlinePlus

Scanning electron micrographs of (A) B. longum subsp. infantis UMA299, (B) unfilled alginate bead, (C) unfilled chitosan-coated alginate bead, (D) B. longum subsp. longum UMA 306, (E) alginate bead containing B. longum subsp. longum UMA 401, (F) chitosan-coated alginate bead containing B. longum subsp. longum UMA 300. Samples were dried before sputter-coating with gold. SEM was set at high-vacuum, 10 kV.
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Figure 2: Scanning electron micrographs of (A) B. longum subsp. infantis UMA299, (B) unfilled alginate bead, (C) unfilled chitosan-coated alginate bead, (D) B. longum subsp. longum UMA 306, (E) alginate bead containing B. longum subsp. longum UMA 401, (F) chitosan-coated alginate bead containing B. longum subsp. longum UMA 300. Samples were dried before sputter-coating with gold. SEM was set at high-vacuum, 10 kV.

Mentions: Scanning electron microscopy (SEM) was used to inspect the structure of the alginate and chitosan-coated alginate beads (Figure 2). Freeze-dried microgels were uniform in size and shape. However, the surfaces of the microgels observed by SEM appeared wrinkled, whereas they presented as smooth when observed by optical microscopy. This is likely due to sublimation of water originally trapped within the hydrogel matrix, as has been described previously (Yeung et al., 2016). The chitosan-coated alginate beads appeared to be more irregular in shape compared to alginate beads. Qualitatively, the alginate beads had smoother wrinkles and microstructures, whereas the chitosan-coated beads exhibited sharp jagged edges. This observation suggests that the chitosan layer has been successfully deposited onto the external surfaces of the alginate microgels.


Microencapsulation in Alginate and Chitosan Microgels to Enhance Viability of Bifidobacterium longum for Oral Delivery.

Yeung TW, Üçok EF, Tiani KA, McClements DJ, Sela DA - Front Microbiol (2016)

Scanning electron micrographs of (A) B. longum subsp. infantis UMA299, (B) unfilled alginate bead, (C) unfilled chitosan-coated alginate bead, (D) B. longum subsp. longum UMA 306, (E) alginate bead containing B. longum subsp. longum UMA 401, (F) chitosan-coated alginate bead containing B. longum subsp. longum UMA 300. Samples were dried before sputter-coating with gold. SEM was set at high-vacuum, 10 kV.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4835488&req=5

Figure 2: Scanning electron micrographs of (A) B. longum subsp. infantis UMA299, (B) unfilled alginate bead, (C) unfilled chitosan-coated alginate bead, (D) B. longum subsp. longum UMA 306, (E) alginate bead containing B. longum subsp. longum UMA 401, (F) chitosan-coated alginate bead containing B. longum subsp. longum UMA 300. Samples were dried before sputter-coating with gold. SEM was set at high-vacuum, 10 kV.
Mentions: Scanning electron microscopy (SEM) was used to inspect the structure of the alginate and chitosan-coated alginate beads (Figure 2). Freeze-dried microgels were uniform in size and shape. However, the surfaces of the microgels observed by SEM appeared wrinkled, whereas they presented as smooth when observed by optical microscopy. This is likely due to sublimation of water originally trapped within the hydrogel matrix, as has been described previously (Yeung et al., 2016). The chitosan-coated alginate beads appeared to be more irregular in shape compared to alginate beads. Qualitatively, the alginate beads had smoother wrinkles and microstructures, whereas the chitosan-coated beads exhibited sharp jagged edges. This observation suggests that the chitosan layer has been successfully deposited onto the external surfaces of the alginate microgels.

Bottom Line: Encapsulated obligate anaerobes Bifidobacterium longum subsp. infantis and Bifidobacterium longum subsp. longum exhibited differences in viability in a strain-dependent manner, without a discernable relationship to subspecies lineage.Coating alginate microgels with chitosan did not improve viability compared to cells encapsulated in alginate microgels alone, suggesting that modifying the surface charge alone does not enhance delivery.Thus hydrogel beads have great potential for improving the stability and efficacy of bifidobacterial probiotics in various nutritional interventions.

View Article: PubMed Central - PubMed

Affiliation: Department of Food Science, University of Massachusetts Amherst, MA, USA.

ABSTRACT
Probiotic microorganisms are incorporated into a wide variety of foods, supplements, and pharmaceuticals to promote human health and wellness. However, maintaining bacterial cell viability during storage and gastrointestinal transit remains a challenge. Encapsulation of bifidobacteria within food-grade hydrogel particles potentially mitigates their sensitivity to environmental stresses. In this study, Bifidobacterium longum subspecies and strains were encapsulated in core-shell microgels consisting of an alginate core and a microgel shell. Encapsulated obligate anaerobes Bifidobacterium longum subsp. infantis and Bifidobacterium longum subsp. longum exhibited differences in viability in a strain-dependent manner, without a discernable relationship to subspecies lineage. This includes viability under aerobic storage conditions and modeled gastrointestinal tract conditions. Coating alginate microgels with chitosan did not improve viability compared to cells encapsulated in alginate microgels alone, suggesting that modifying the surface charge alone does not enhance delivery. Thus hydrogel beads have great potential for improving the stability and efficacy of bifidobacterial probiotics in various nutritional interventions.

No MeSH data available.


Related in: MedlinePlus